A [3Fe-4S] cluster is required for tRNA thiolation in archaea and eukaryotes

Yuchen Liu, David J. Vinyard, Megan E. Reesbeck, Tateki Suzuki, Kasidet Manakongtreecheep, Patrick L. Holland, Gary W Brudvig, Dieter Söll

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The sulfur-containing nucleosides in transfer RNA (tRNAs) are present in all three domains of life; they have critical functions for accurate and efficient translation, such as tRNA structure stabilization and proper codon recognition. The tRNA modification enzymes ThiI (in bacteria and archaea) and Ncs6 (in archaea and eukaryotic cytosols) catalyze the formation of 4-thiouridine (s4U) and 2-thiouridine (s2U), respectively. The ThiI homologs were proposed to transfer sulfur via cysteine persulfide enzyme adducts, whereas the reaction mechanism of Ncs6 remains unknown. Here we show that ThiI from the archaeon Methanococcus maripaludis contains a [3Fe-4S] cluster that is essential for its tRNA thiolation activity. Furthermore, the archaeal and eukaryotic Ncs6 homologs as well as phosphoseryl-tRNA (Sep-tRNA):Cys-tRNA synthase (SepCysS), which catalyzes the Sep-tRNA to Cys-tRNA conversion in methanogens, also possess a [3Fe-4S] cluster similar to the methanogenic archaeal ThiI. These results suggest that the diverse tRNA thiolation processes in archaea and eukaryotic cytosols share a common mechanism dependent on a [3Fe-4S] cluster for sulfur transfer.

Original languageEnglish
Pages (from-to)12703-12708
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number45
DOIs
Publication statusPublished - Nov 8 2016

Fingerprint

Transfer RNA
RNA, Transfer, Cys
Sulfur
Thiouridine
Methanogens
Enzymes
Nucleosides
Bacteria
Stabilization

Keywords

  • CTU1
  • Iron-sulfur cluster
  • Thionucleosides
  • TRNA modification

ASJC Scopus subject areas

  • General

Cite this

Liu, Y., Vinyard, D. J., Reesbeck, M. E., Suzuki, T., Manakongtreecheep, K., Holland, P. L., ... Söll, D. (2016). A [3Fe-4S] cluster is required for tRNA thiolation in archaea and eukaryotes. Proceedings of the National Academy of Sciences of the United States of America, 113(45), 12703-12708. https://doi.org/10.1073/pnas.1615732113

A [3Fe-4S] cluster is required for tRNA thiolation in archaea and eukaryotes. / Liu, Yuchen; Vinyard, David J.; Reesbeck, Megan E.; Suzuki, Tateki; Manakongtreecheep, Kasidet; Holland, Patrick L.; Brudvig, Gary W; Söll, Dieter.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, No. 45, 08.11.2016, p. 12703-12708.

Research output: Contribution to journalArticle

Liu, Y, Vinyard, DJ, Reesbeck, ME, Suzuki, T, Manakongtreecheep, K, Holland, PL, Brudvig, GW & Söll, D 2016, 'A [3Fe-4S] cluster is required for tRNA thiolation in archaea and eukaryotes', Proceedings of the National Academy of Sciences of the United States of America, vol. 113, no. 45, pp. 12703-12708. https://doi.org/10.1073/pnas.1615732113
Liu, Yuchen ; Vinyard, David J. ; Reesbeck, Megan E. ; Suzuki, Tateki ; Manakongtreecheep, Kasidet ; Holland, Patrick L. ; Brudvig, Gary W ; Söll, Dieter. / A [3Fe-4S] cluster is required for tRNA thiolation in archaea and eukaryotes. In: Proceedings of the National Academy of Sciences of the United States of America. 2016 ; Vol. 113, No. 45. pp. 12703-12708.
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