A designed buried salt bridge modulates heterodimerization of a membrane peptide

Sandip Shinde, Jennifer K. Binder, Bhupesh Goyal, B. Woodrum, Sonia De Munari, Marcia Levitus, Giovanna Ghirlanda

Research output: Contribution to journalArticle

Abstract

Specific helix-helix interactions underpin the correct assembly of multipass membrane proteins. Here, we show that a designed buried salt bridge mediates heterodimer formation of model transmembrane helical peptides in a pH-dependent manner. The model peptides bear side chains functionalized with either a carboxylic acid or a primary amine within a hydrophobic segment. The association behavior was monitored by Forster resonance energy transfer, revealing that heterodimer formation is maximized at a pH close to neutrality (pH 6.5), at which each peptide is found in a charged state. In contrast, heterodimerization is disfavored at low and high values of pH, because either the carboxylic acid or the primary amine is present in its neutral state, thus preventing the formation of a salt bridge. These findings provide a blueprint for the design and modulation of protein-protein interactions in membrane proteins.

Original languageEnglish
Pages (from-to)437-443
Number of pages7
JournalBiopolymers
Volume102
Issue number6
DOIs
Publication statusPublished - Nov 1 2014

Fingerprint

Peptides
Salts
Carboxylic Acids
Proteins
Membranes
Amines
Membrane Proteins
Carboxylic acids
Blueprints
Fluorescence Resonance Energy Transfer
Modulation

Keywords

  • Dimerization
  • Förster resonance energy transfer
  • Heterodimer
  • Membrane protein
  • Micelles

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biomaterials
  • Organic Chemistry

Cite this

Shinde, S., Binder, J. K., Goyal, B., Woodrum, B., De Munari, S., Levitus, M., & Ghirlanda, G. (2014). A designed buried salt bridge modulates heterodimerization of a membrane peptide. Biopolymers, 102(6), 437-443. https://doi.org/10.1002/bip.22564

A designed buried salt bridge modulates heterodimerization of a membrane peptide. / Shinde, Sandip; Binder, Jennifer K.; Goyal, Bhupesh; Woodrum, B.; De Munari, Sonia; Levitus, Marcia; Ghirlanda, Giovanna.

In: Biopolymers, Vol. 102, No. 6, 01.11.2014, p. 437-443.

Research output: Contribution to journalArticle

Shinde, S, Binder, JK, Goyal, B, Woodrum, B, De Munari, S, Levitus, M & Ghirlanda, G 2014, 'A designed buried salt bridge modulates heterodimerization of a membrane peptide', Biopolymers, vol. 102, no. 6, pp. 437-443. https://doi.org/10.1002/bip.22564
Shinde S, Binder JK, Goyal B, Woodrum B, De Munari S, Levitus M et al. A designed buried salt bridge modulates heterodimerization of a membrane peptide. Biopolymers. 2014 Nov 1;102(6):437-443. https://doi.org/10.1002/bip.22564
Shinde, Sandip ; Binder, Jennifer K. ; Goyal, Bhupesh ; Woodrum, B. ; De Munari, Sonia ; Levitus, Marcia ; Ghirlanda, Giovanna. / A designed buried salt bridge modulates heterodimerization of a membrane peptide. In: Biopolymers. 2014 ; Vol. 102, No. 6. pp. 437-443.
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