A DNA tweezer-actuated enzyme nanoreactor

Minghui Liu, Jinglin Fu, Christian Hejesen, Yuhe Yang, Neal W. Woodbury, Kurt Gothelf, Yan Liu, Hao Yan

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

The functions of regulatory enzymes are essential to modulating cellular pathways. Here we report a tweezer-like DNA nanodevice to actuate the activity of an enzyme/cofactor pair. A dehydrogenase and NAD + cofactor are attached to different arms of the DNA tweezer structure and actuation of enzymatic function is achieved by switching the tweezers between open and closed states. The enzyme/cofactor pair is spatially separated in the open state with inhibited enzyme function, whereas in the closed state, enzyme is activated by the close proximity of the two molecules. The conformational state of the DNA tweezer is controlled by the addition of specific oligonucleotides that serve as the thermodynamic driver (fuel) to trigger the change. Using this approach, several cycles of externally controlled enzyme inhibition and activation are successfully demonstrated. This principle of responsive enzyme nanodevices may be used to regulate other types of enzymes and to introduce feedback or feed-forward control loops.

Original languageEnglish
Article number2127
JournalNature Communications
Volume4
DOIs
Publication statusPublished - 2013

Fingerprint

Nanoreactors
enzymes
deoxyribonucleic acid
DNA
Enzymes
Coenzymes
Enzyme inhibition
Enzyme Activation
Feedforward control
Thermodynamics
Oligonucleotides
NAD
feedforward control
Oxidoreductases
Chemical activation
dehydrogenases
oligonucleotides
Feedback
actuation
Molecules

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Liu, M., Fu, J., Hejesen, C., Yang, Y., Woodbury, N. W., Gothelf, K., ... Yan, H. (2013). A DNA tweezer-actuated enzyme nanoreactor. Nature Communications, 4, [2127]. https://doi.org/10.1038/ncomms3127

A DNA tweezer-actuated enzyme nanoreactor. / Liu, Minghui; Fu, Jinglin; Hejesen, Christian; Yang, Yuhe; Woodbury, Neal W.; Gothelf, Kurt; Liu, Yan; Yan, Hao.

In: Nature Communications, Vol. 4, 2127, 2013.

Research output: Contribution to journalArticle

Liu, M, Fu, J, Hejesen, C, Yang, Y, Woodbury, NW, Gothelf, K, Liu, Y & Yan, H 2013, 'A DNA tweezer-actuated enzyme nanoreactor', Nature Communications, vol. 4, 2127. https://doi.org/10.1038/ncomms3127
Liu M, Fu J, Hejesen C, Yang Y, Woodbury NW, Gothelf K et al. A DNA tweezer-actuated enzyme nanoreactor. Nature Communications. 2013;4. 2127. https://doi.org/10.1038/ncomms3127
Liu, Minghui ; Fu, Jinglin ; Hejesen, Christian ; Yang, Yuhe ; Woodbury, Neal W. ; Gothelf, Kurt ; Liu, Yan ; Yan, Hao. / A DNA tweezer-actuated enzyme nanoreactor. In: Nature Communications. 2013 ; Vol. 4.
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