A guide to electron paramagnetic resonance spectroscopy of Photosystem II membranes

Anne Frances Miller, Gary W Brudvig

Research output: Contribution to journalArticle

214 Citations (Scopus)

Abstract

This guide is intended to aid in the detection and identification of paramagnetic species in Photosystem II membranes, by electron paramagnetic resonance spectroscopy. The spectral features and occurrence of each of the electron paramagnetic resonance signals from Photosystem II are discussed, in relation to the nature of the moiety giving rise to the signal and the role of that species in photosynthetic electron transport. Examples of most of the signals discussed are shown. The electron paramagnetic resonance signals produced by the cytochrome b6f and Photosystem I complexes, as well as the signals from other common contaminants, are also reviewed. Furthermore, references to seminal experiments on bacterial reaction centers are included. By reviewing both the spectroscopic and biochemical bases for the electron paramagnetic resonance signals of the cofactors that mediate photosynthetic electron transport, this paper provides an introduction to the use and interpretation of electron paramagnetic resonance spectroscopy in the study of Photosystem II.

Original languageEnglish
Pages (from-to)1-18
Number of pages18
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1056
Issue number1
DOIs
Publication statusPublished - Jan 3 1991

Fingerprint

Photosystem II Protein Complex
Electron Spin Resonance Spectroscopy
Paramagnetic resonance
Spectrum Analysis
Spectroscopy
Membranes
Electron Transport
Cytochrome b6f Complex
Photosystem I Protein Complex
Impurities
Experiments

Keywords

  • EPR
  • Photosystem II

ASJC Scopus subject areas

  • Biophysics

Cite this

A guide to electron paramagnetic resonance spectroscopy of Photosystem II membranes. / Miller, Anne Frances; Brudvig, Gary W.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1056, No. 1, 03.01.1991, p. 1-18.

Research output: Contribution to journalArticle

@article{fd4bf9032d1945719cf18e9aa1700bba,
title = "A guide to electron paramagnetic resonance spectroscopy of Photosystem II membranes",
abstract = "This guide is intended to aid in the detection and identification of paramagnetic species in Photosystem II membranes, by electron paramagnetic resonance spectroscopy. The spectral features and occurrence of each of the electron paramagnetic resonance signals from Photosystem II are discussed, in relation to the nature of the moiety giving rise to the signal and the role of that species in photosynthetic electron transport. Examples of most of the signals discussed are shown. The electron paramagnetic resonance signals produced by the cytochrome b6f and Photosystem I complexes, as well as the signals from other common contaminants, are also reviewed. Furthermore, references to seminal experiments on bacterial reaction centers are included. By reviewing both the spectroscopic and biochemical bases for the electron paramagnetic resonance signals of the cofactors that mediate photosynthetic electron transport, this paper provides an introduction to the use and interpretation of electron paramagnetic resonance spectroscopy in the study of Photosystem II.",
keywords = "EPR, Photosystem II",
author = "Miller, {Anne Frances} and Brudvig, {Gary W}",
year = "1991",
month = "1",
day = "3",
doi = "10.1016/S0005-2728(05)80067-2",
language = "English",
volume = "1056",
pages = "1--18",
journal = "Biochimica et Biophysica Acta - Bioenergetics",
issn = "0005-2728",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - A guide to electron paramagnetic resonance spectroscopy of Photosystem II membranes

AU - Miller, Anne Frances

AU - Brudvig, Gary W

PY - 1991/1/3

Y1 - 1991/1/3

N2 - This guide is intended to aid in the detection and identification of paramagnetic species in Photosystem II membranes, by electron paramagnetic resonance spectroscopy. The spectral features and occurrence of each of the electron paramagnetic resonance signals from Photosystem II are discussed, in relation to the nature of the moiety giving rise to the signal and the role of that species in photosynthetic electron transport. Examples of most of the signals discussed are shown. The electron paramagnetic resonance signals produced by the cytochrome b6f and Photosystem I complexes, as well as the signals from other common contaminants, are also reviewed. Furthermore, references to seminal experiments on bacterial reaction centers are included. By reviewing both the spectroscopic and biochemical bases for the electron paramagnetic resonance signals of the cofactors that mediate photosynthetic electron transport, this paper provides an introduction to the use and interpretation of electron paramagnetic resonance spectroscopy in the study of Photosystem II.

AB - This guide is intended to aid in the detection and identification of paramagnetic species in Photosystem II membranes, by electron paramagnetic resonance spectroscopy. The spectral features and occurrence of each of the electron paramagnetic resonance signals from Photosystem II are discussed, in relation to the nature of the moiety giving rise to the signal and the role of that species in photosynthetic electron transport. Examples of most of the signals discussed are shown. The electron paramagnetic resonance signals produced by the cytochrome b6f and Photosystem I complexes, as well as the signals from other common contaminants, are also reviewed. Furthermore, references to seminal experiments on bacterial reaction centers are included. By reviewing both the spectroscopic and biochemical bases for the electron paramagnetic resonance signals of the cofactors that mediate photosynthetic electron transport, this paper provides an introduction to the use and interpretation of electron paramagnetic resonance spectroscopy in the study of Photosystem II.

KW - EPR

KW - Photosystem II

UR - http://www.scopus.com/inward/record.url?scp=0026025417&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026025417&partnerID=8YFLogxK

U2 - 10.1016/S0005-2728(05)80067-2

DO - 10.1016/S0005-2728(05)80067-2

M3 - Article

VL - 1056

SP - 1

EP - 18

JO - Biochimica et Biophysica Acta - Bioenergetics

JF - Biochimica et Biophysica Acta - Bioenergetics

SN - 0005-2728

IS - 1

ER -