A heterogeneous PNA-based SERS method for DNA detection

Laura Fabris, Mark Dante, Gary Braun, Joon Lee Seung, Norbert O. Reich, Martin Moskovits, Thuc Quyen Nguyen, Guillermo C. Bazan

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

A simple method for ssDNA detection based on SERS signals and PNA slides is reported. Upon hybridization with ssDNA, the surface charge of the PNA slides changes from neutral to negative. Subsequent treatment with partly aggregated, positively charged silver nanoparticles results in selective electrostatic adsorption onto surfaces containing PNA/ssDNA duplexes. Addition of rhodamine6G gives rise to SERS signals characteristic of this dye, which are diagnostic of the hybridization event. Characterization by SERS maps and AFM reveals that the distribution of nanoparticles is random and that approximately 1 in 10 aggregate sites is responsible for the SERS spectra.

Original languageEnglish
Pages (from-to)6086-6087
Number of pages2
JournalJournal of the American Chemical Society
Volume129
Issue number19
DOIs
Publication statusPublished - May 16 2007

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Nanoparticles
DNA
Surface charge
Static Electricity
Silver
Adsorption
Electrostatics
Coloring Agents
Dyes

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Fabris, L., Dante, M., Braun, G., Seung, J. L., Reich, N. O., Moskovits, M., ... Bazan, G. C. (2007). A heterogeneous PNA-based SERS method for DNA detection. Journal of the American Chemical Society, 129(19), 6086-6087. https://doi.org/10.1021/ja0705184

A heterogeneous PNA-based SERS method for DNA detection. / Fabris, Laura; Dante, Mark; Braun, Gary; Seung, Joon Lee; Reich, Norbert O.; Moskovits, Martin; Nguyen, Thuc Quyen; Bazan, Guillermo C.

In: Journal of the American Chemical Society, Vol. 129, No. 19, 16.05.2007, p. 6086-6087.

Research output: Contribution to journalArticle

Fabris, L, Dante, M, Braun, G, Seung, JL, Reich, NO, Moskovits, M, Nguyen, TQ & Bazan, GC 2007, 'A heterogeneous PNA-based SERS method for DNA detection', Journal of the American Chemical Society, vol. 129, no. 19, pp. 6086-6087. https://doi.org/10.1021/ja0705184
Fabris L, Dante M, Braun G, Seung JL, Reich NO, Moskovits M et al. A heterogeneous PNA-based SERS method for DNA detection. Journal of the American Chemical Society. 2007 May 16;129(19):6086-6087. https://doi.org/10.1021/ja0705184
Fabris, Laura ; Dante, Mark ; Braun, Gary ; Seung, Joon Lee ; Reich, Norbert O. ; Moskovits, Martin ; Nguyen, Thuc Quyen ; Bazan, Guillermo C. / A heterogeneous PNA-based SERS method for DNA detection. In: Journal of the American Chemical Society. 2007 ; Vol. 129, No. 19. pp. 6086-6087.
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