A heterogeneous PNA-based SERS method for DNA detection

Laura Fabris; Mark Dante; Gary Braun; Joon Lee Seung; Norbert O. Reich; Martin Moskovits; Thuc Quyen Nguyen; Guillermo C. Bazan

doi:10.1021/ja0705184

A heterogeneous PNA-based SERS method for DNA detection

Laura Fabris, Mark Dante, Gary Braun, Joon Lee Seung, Norbert O. Reich, Martin Moskovits, Thuc Quyen Nguyen, Guillermo C. Bazan

Rutgers University

Research output: Contribution to journal › Article › peer-review

131 Citations (Scopus)

Abstract

A simple method for ssDNA detection based on SERS signals and PNA slides is reported. Upon hybridization with ssDNA, the surface charge of the PNA slides changes from neutral to negative. Subsequent treatment with partly aggregated, positively charged silver nanoparticles results in selective electrostatic adsorption onto surfaces containing PNA/ssDNA duplexes. Addition of rhodamine6G gives rise to SERS signals characteristic of this dye, which are diagnostic of the hybridization event. Characterization by SERS maps and AFM reveals that the distribution of nanoparticles is random and that approximately 1 in 10 aggregate sites is responsible for the SERS spectra.

Original language	English
Pages (from-to)	6086-6087
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	129
Issue number	19
DOIs	https://doi.org/10.1021/ja0705184
Publication status	Published - May 16 2007

ASJC Scopus subject areas

Catalysis
Chemistry(all)
Biochemistry
Colloid and Surface Chemistry

Access to Document

10.1021/ja0705184

Fingerprint Dive into the research topics of 'A heterogeneous PNA-based SERS method for DNA detection'. Together they form a unique fingerprint.

Cite this

@article{94e9dc6e4fcd496188d15c3c0e7cceaf,

title = "A heterogeneous PNA-based SERS method for DNA detection",

abstract = "A simple method for ssDNA detection based on SERS signals and PNA slides is reported. Upon hybridization with ssDNA, the surface charge of the PNA slides changes from neutral to negative. Subsequent treatment with partly aggregated, positively charged silver nanoparticles results in selective electrostatic adsorption onto surfaces containing PNA/ssDNA duplexes. Addition of rhodamine6G gives rise to SERS signals characteristic of this dye, which are diagnostic of the hybridization event. Characterization by SERS maps and AFM reveals that the distribution of nanoparticles is random and that approximately 1 in 10 aggregate sites is responsible for the SERS spectra.",

author = "Laura Fabris and Mark Dante and Gary Braun and Seung, {Joon Lee} and Reich, {Norbert O.} and Martin Moskovits and Nguyen, {Thuc Quyen} and Bazan, {Guillermo C.}",

year = "2007",

month = may,

day = "16",

doi = "10.1021/ja0705184",

language = "English",

volume = "129",

pages = "6086--6087",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

number = "19",

}

TY - JOUR

T1 - A heterogeneous PNA-based SERS method for DNA detection

AU - Fabris, Laura

AU - Dante, Mark

AU - Braun, Gary

AU - Seung, Joon Lee

AU - Reich, Norbert O.

AU - Moskovits, Martin

AU - Nguyen, Thuc Quyen

AU - Bazan, Guillermo C.

PY - 2007/5/16

Y1 - 2007/5/16

N2 - A simple method for ssDNA detection based on SERS signals and PNA slides is reported. Upon hybridization with ssDNA, the surface charge of the PNA slides changes from neutral to negative. Subsequent treatment with partly aggregated, positively charged silver nanoparticles results in selective electrostatic adsorption onto surfaces containing PNA/ssDNA duplexes. Addition of rhodamine6G gives rise to SERS signals characteristic of this dye, which are diagnostic of the hybridization event. Characterization by SERS maps and AFM reveals that the distribution of nanoparticles is random and that approximately 1 in 10 aggregate sites is responsible for the SERS spectra.

AB - A simple method for ssDNA detection based on SERS signals and PNA slides is reported. Upon hybridization with ssDNA, the surface charge of the PNA slides changes from neutral to negative. Subsequent treatment with partly aggregated, positively charged silver nanoparticles results in selective electrostatic adsorption onto surfaces containing PNA/ssDNA duplexes. Addition of rhodamine6G gives rise to SERS signals characteristic of this dye, which are diagnostic of the hybridization event. Characterization by SERS maps and AFM reveals that the distribution of nanoparticles is random and that approximately 1 in 10 aggregate sites is responsible for the SERS spectra.

UR - http://www.scopus.com/inward/record.url?scp=34249085902&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34249085902&partnerID=8YFLogxK

U2 - 10.1021/ja0705184

DO - 10.1021/ja0705184

M3 - Article

C2 - 17451246

AN - SCOPUS:34249085902

VL - 129

SP - 6086

EP - 6087

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 19

ER -