A heterogeneous PNA-based SERS method for DNA detection

Laura Fabris, Mark Dante, Gary Braun, Joon Lee Seung, Norbert O. Reich, Martin Moskovits, Thuc Quyen Nguyen, Guillermo C. Bazan

Research output: Contribution to journalArticlepeer-review

131 Citations (Scopus)


A simple method for ssDNA detection based on SERS signals and PNA slides is reported. Upon hybridization with ssDNA, the surface charge of the PNA slides changes from neutral to negative. Subsequent treatment with partly aggregated, positively charged silver nanoparticles results in selective electrostatic adsorption onto surfaces containing PNA/ssDNA duplexes. Addition of rhodamine6G gives rise to SERS signals characteristic of this dye, which are diagnostic of the hybridization event. Characterization by SERS maps and AFM reveals that the distribution of nanoparticles is random and that approximately 1 in 10 aggregate sites is responsible for the SERS spectra.

Original languageEnglish
Pages (from-to)6086-6087
Number of pages2
JournalJournal of the American Chemical Society
Issue number19
Publication statusPublished - May 16 2007

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Fingerprint Dive into the research topics of 'A heterogeneous PNA-based SERS method for DNA detection'. Together they form a unique fingerprint.

Cite this