Abstract
A simple method for ssDNA detection based on SERS signals and PNA slides is reported. Upon hybridization with ssDNA, the surface charge of the PNA slides changes from neutral to negative. Subsequent treatment with partly aggregated, positively charged silver nanoparticles results in selective electrostatic adsorption onto surfaces containing PNA/ssDNA duplexes. Addition of rhodamine6G gives rise to SERS signals characteristic of this dye, which are diagnostic of the hybridization event. Characterization by SERS maps and AFM reveals that the distribution of nanoparticles is random and that approximately 1 in 10 aggregate sites is responsible for the SERS spectra.
| Original language | English |
|---|---|
| Pages (from-to) | 6086-6087 |
| Number of pages | 2 |
| Journal | Journal of the American Chemical Society |
| Volume | 129 |
| Issue number | 19 |
| DOIs | |
| Publication status | Published - May 16 2007 |
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ASJC Scopus subject areas
- Chemistry(all)
Cite this
A heterogeneous PNA-based SERS method for DNA detection. / Fabris, Laura; Dante, Mark; Braun, Gary; Seung, Joon Lee; Reich, Norbert O.; Moskovits, Martin; Nguyen, Thuc Quyen; Bazan, Guillermo C.
In: Journal of the American Chemical Society, Vol. 129, No. 19, 16.05.2007, p. 6086-6087.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A heterogeneous PNA-based SERS method for DNA detection
AU - Fabris, Laura
AU - Dante, Mark
AU - Braun, Gary
AU - Seung, Joon Lee
AU - Reich, Norbert O.
AU - Moskovits, Martin
AU - Nguyen, Thuc Quyen
AU - Bazan, Guillermo C.
PY - 2007/5/16
Y1 - 2007/5/16
N2 - A simple method for ssDNA detection based on SERS signals and PNA slides is reported. Upon hybridization with ssDNA, the surface charge of the PNA slides changes from neutral to negative. Subsequent treatment with partly aggregated, positively charged silver nanoparticles results in selective electrostatic adsorption onto surfaces containing PNA/ssDNA duplexes. Addition of rhodamine6G gives rise to SERS signals characteristic of this dye, which are diagnostic of the hybridization event. Characterization by SERS maps and AFM reveals that the distribution of nanoparticles is random and that approximately 1 in 10 aggregate sites is responsible for the SERS spectra.
AB - A simple method for ssDNA detection based on SERS signals and PNA slides is reported. Upon hybridization with ssDNA, the surface charge of the PNA slides changes from neutral to negative. Subsequent treatment with partly aggregated, positively charged silver nanoparticles results in selective electrostatic adsorption onto surfaces containing PNA/ssDNA duplexes. Addition of rhodamine6G gives rise to SERS signals characteristic of this dye, which are diagnostic of the hybridization event. Characterization by SERS maps and AFM reveals that the distribution of nanoparticles is random and that approximately 1 in 10 aggregate sites is responsible for the SERS spectra.
UR - http://www.scopus.com/inward/record.url?scp=34249085902&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34249085902&partnerID=8YFLogxK
U2 - 10.1021/ja0705184
DO - 10.1021/ja0705184
M3 - Article
VL - 129
SP - 6086
EP - 6087
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
SN - 0002-7863
IS - 19
ER -