A hybrid nanofiber matrix to control the survival and maturation of brain neurons

Shantanu Sur, Eugene T. Pashuck, Mustafa O. Guler, Masao Ito, Samuel I Stupp, Thomas Launey

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Scaffold design plays a crucial role in developing graft-based regenerative strategies, especially when intended to be used in a highly ordered nerve tissue. Here we describe a hybrid matrix approach, which combines the structural properties of collagen (type I) with the epitope-presenting ability of peptide amphiphile (PA) nanofibers. Self-assembly of PA and collagen molecules results in a nanofibrous scaffold with homogeneous fiber diameter of 20-30 nm, where the number of laminin epitopes IKVAV and YIGSR can be varied by changing the PA concentrations over a broad range of 0.125-2 mg/ml. Granule cells (GC) and Purkinje cells (PC), two major neuronal subtypes of cerebellar cortex, demonstrate distinct response to this change of epitope concentration. On IKVAV hybrid constructs, GC density increases three-fold compared with the control collagen substrate at a PA concentration of ≥0.25 mg/ml, while PC density reaches a maximum (five-fold vs. control) at 0.25 mg/ml of PA and rapidly decreases at higher PA concentrations. In addition, adjustment of the epitope number allowed us to achieve fine control over PC dendrite and axon growth. Due to the ability to modulate neuron survival and maturation by easy manipulation of epitope density, our design offers a versatile test bed to study the extracellular matrix (ECM) contribution in neuron development and the design of optimal neuronal scaffold biomaterials.

Original languageEnglish
Pages (from-to)545-555
Number of pages11
JournalBiomaterials
Volume33
Issue number2
DOIs
Publication statusPublished - Jan 2012

Fingerprint

Nanofibers
Amphiphiles
Epitopes
Peptides
Neurons
Brain
isoleucyl-lysyl-valyl-alanyl-valine
Purkinje Cells
Collagen
Scaffolds (biology)
Scaffolds
tyrosyl-isoleucyl-glycyl-seryl-arginine
Cell Count
Nerve Tissue
Cerebellar Cortex
Biocompatible Materials
Laminin
Dendrites
Collagen Type I
Biomaterials

Keywords

  • Brain
  • Collagen
  • Laminin
  • Nerve tissue engineering
  • Peptide amphiphile
  • Self assembly

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

A hybrid nanofiber matrix to control the survival and maturation of brain neurons. / Sur, Shantanu; Pashuck, Eugene T.; Guler, Mustafa O.; Ito, Masao; Stupp, Samuel I; Launey, Thomas.

In: Biomaterials, Vol. 33, No. 2, 01.2012, p. 545-555.

Research output: Contribution to journalArticle

Sur, Shantanu ; Pashuck, Eugene T. ; Guler, Mustafa O. ; Ito, Masao ; Stupp, Samuel I ; Launey, Thomas. / A hybrid nanofiber matrix to control the survival and maturation of brain neurons. In: Biomaterials. 2012 ; Vol. 33, No. 2. pp. 545-555.
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