A replicable tetrahedral nanostructure self-assembled from a single DNA strand

Zhe Li, Bryan Wei, Jeanette Nangreave, Chenxiang Lin, Yan Liu, Yongli Mi, Hao Yan

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

We report the design and construction of a nanometer-sized tetrahedron from a single strand of DNA that is 286 nucleotides long. The formation of the tetrahedron was verified by restriction enzyme digestion, Ferguson analysis, and atomic force microscopy (AFM) imaging. We further demonstrate that synthesis of the tetrahedron can be easily scaled up through in vivo replication using standard molecular cloning techniques. We found that the in vivo replication efficiency of the tetrahedron is significantly higher in comparison to in vitro replication using rolling-circle amplification (RCA). Our results suggest that it is now possible to design and replicate increasingly complex, single-stranded DNA nanostructures in vivo.

Original languageEnglish
Pages (from-to)13093-13098
Number of pages6
JournalJournal of the American Chemical Society
Volume131
Issue number36
DOIs
Publication statusPublished - Sep 16 2009

Fingerprint

Nanostructures
Atomic Force Microscopy
Single-Stranded DNA
Molecular Cloning
Digestion
DNA
Nucleotides
Cloning
Enzymes
Amplification
Atomic force microscopy
Imaging techniques
In Vitro Techniques

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

A replicable tetrahedral nanostructure self-assembled from a single DNA strand. / Li, Zhe; Wei, Bryan; Nangreave, Jeanette; Lin, Chenxiang; Liu, Yan; Mi, Yongli; Yan, Hao.

In: Journal of the American Chemical Society, Vol. 131, No. 36, 16.09.2009, p. 13093-13098.

Research output: Contribution to journalArticle

Li, Zhe ; Wei, Bryan ; Nangreave, Jeanette ; Lin, Chenxiang ; Liu, Yan ; Mi, Yongli ; Yan, Hao. / A replicable tetrahedral nanostructure self-assembled from a single DNA strand. In: Journal of the American Chemical Society. 2009 ; Vol. 131, No. 36. pp. 13093-13098.
@article{f91cdb8970eb4b75bc3d7632f1c8919c,
title = "A replicable tetrahedral nanostructure self-assembled from a single DNA strand",
abstract = "We report the design and construction of a nanometer-sized tetrahedron from a single strand of DNA that is 286 nucleotides long. The formation of the tetrahedron was verified by restriction enzyme digestion, Ferguson analysis, and atomic force microscopy (AFM) imaging. We further demonstrate that synthesis of the tetrahedron can be easily scaled up through in vivo replication using standard molecular cloning techniques. We found that the in vivo replication efficiency of the tetrahedron is significantly higher in comparison to in vitro replication using rolling-circle amplification (RCA). Our results suggest that it is now possible to design and replicate increasingly complex, single-stranded DNA nanostructures in vivo.",
author = "Zhe Li and Bryan Wei and Jeanette Nangreave and Chenxiang Lin and Yan Liu and Yongli Mi and Hao Yan",
year = "2009",
month = "9",
day = "16",
doi = "10.1021/ja903768f",
language = "English",
volume = "131",
pages = "13093--13098",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "36",

}

TY - JOUR

T1 - A replicable tetrahedral nanostructure self-assembled from a single DNA strand

AU - Li, Zhe

AU - Wei, Bryan

AU - Nangreave, Jeanette

AU - Lin, Chenxiang

AU - Liu, Yan

AU - Mi, Yongli

AU - Yan, Hao

PY - 2009/9/16

Y1 - 2009/9/16

N2 - We report the design and construction of a nanometer-sized tetrahedron from a single strand of DNA that is 286 nucleotides long. The formation of the tetrahedron was verified by restriction enzyme digestion, Ferguson analysis, and atomic force microscopy (AFM) imaging. We further demonstrate that synthesis of the tetrahedron can be easily scaled up through in vivo replication using standard molecular cloning techniques. We found that the in vivo replication efficiency of the tetrahedron is significantly higher in comparison to in vitro replication using rolling-circle amplification (RCA). Our results suggest that it is now possible to design and replicate increasingly complex, single-stranded DNA nanostructures in vivo.

AB - We report the design and construction of a nanometer-sized tetrahedron from a single strand of DNA that is 286 nucleotides long. The formation of the tetrahedron was verified by restriction enzyme digestion, Ferguson analysis, and atomic force microscopy (AFM) imaging. We further demonstrate that synthesis of the tetrahedron can be easily scaled up through in vivo replication using standard molecular cloning techniques. We found that the in vivo replication efficiency of the tetrahedron is significantly higher in comparison to in vitro replication using rolling-circle amplification (RCA). Our results suggest that it is now possible to design and replicate increasingly complex, single-stranded DNA nanostructures in vivo.

UR - http://www.scopus.com/inward/record.url?scp=70349134760&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349134760&partnerID=8YFLogxK

U2 - 10.1021/ja903768f

DO - 10.1021/ja903768f

M3 - Article

C2 - 19737020

AN - SCOPUS:70349134760

VL - 131

SP - 13093

EP - 13098

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 36

ER -