A self-assembled nanofiber catalyst for ester hydrolysis

Mustafa O. Guler, Samuel I Stupp

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Catalytic particles based on supramolecular systems have demonstrated significant improvement in the field of catalysis. In this work, histidine-functionalized self-assembling peptide amphiphiles (PAs) were synthesized in order to form self-assembly high-aspect-ratio nanofibers with internal order that can present imidazolyl groups capable of ester hydrolysis. Self-assembly of the molecules making up the catalytic particle was studied by transmission electron microscopy and circular dichroism. The reactive sites were designed with histidine residues to catalyze the hydrolysis of 2,4-dinitrophenyl acetate (DNPA). Enzymatic hydrolysis of DNPA was observed in the presence of the nanofibers by UV-vis spectroscopy and the Michaelis-Menten enzyme kinetics model. Significantly faster hydrolysis rate was observed in the presence of self-assembled nanofibers relative to spherical aggregates expected to have less order as well as single molecule catalysts.

Original languageEnglish
Pages (from-to)12082-12083
Number of pages2
JournalJournal of the American Chemical Society
Volume129
Issue number40
DOIs
Publication statusPublished - Oct 10 2007

Fingerprint

Nanofibers
Hydrolysis
Esters
Histidine
Self assembly
Catalysts
Acetates
Enzyme kinetics
Amphiphiles
Molecules
Enzymatic hydrolysis
Dichroism
Ultraviolet spectroscopy
Peptides
Catalysis
Aspect ratio
Circular Dichroism
Transmission Electron Microscopy
Transmission electron microscopy
Catalytic Domain

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

A self-assembled nanofiber catalyst for ester hydrolysis. / Guler, Mustafa O.; Stupp, Samuel I.

In: Journal of the American Chemical Society, Vol. 129, No. 40, 10.10.2007, p. 12082-12083.

Research output: Contribution to journalArticle

@article{99472f222a544b698f7f22862051d549,
title = "A self-assembled nanofiber catalyst for ester hydrolysis",
abstract = "Catalytic particles based on supramolecular systems have demonstrated significant improvement in the field of catalysis. In this work, histidine-functionalized self-assembling peptide amphiphiles (PAs) were synthesized in order to form self-assembly high-aspect-ratio nanofibers with internal order that can present imidazolyl groups capable of ester hydrolysis. Self-assembly of the molecules making up the catalytic particle was studied by transmission electron microscopy and circular dichroism. The reactive sites were designed with histidine residues to catalyze the hydrolysis of 2,4-dinitrophenyl acetate (DNPA). Enzymatic hydrolysis of DNPA was observed in the presence of the nanofibers by UV-vis spectroscopy and the Michaelis-Menten enzyme kinetics model. Significantly faster hydrolysis rate was observed in the presence of self-assembled nanofibers relative to spherical aggregates expected to have less order as well as single molecule catalysts.",
author = "Guler, {Mustafa O.} and Stupp, {Samuel I}",
year = "2007",
month = "10",
day = "10",
doi = "10.1021/ja075044n",
language = "English",
volume = "129",
pages = "12082--12083",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "40",

}

TY - JOUR

T1 - A self-assembled nanofiber catalyst for ester hydrolysis

AU - Guler, Mustafa O.

AU - Stupp, Samuel I

PY - 2007/10/10

Y1 - 2007/10/10

N2 - Catalytic particles based on supramolecular systems have demonstrated significant improvement in the field of catalysis. In this work, histidine-functionalized self-assembling peptide amphiphiles (PAs) were synthesized in order to form self-assembly high-aspect-ratio nanofibers with internal order that can present imidazolyl groups capable of ester hydrolysis. Self-assembly of the molecules making up the catalytic particle was studied by transmission electron microscopy and circular dichroism. The reactive sites were designed with histidine residues to catalyze the hydrolysis of 2,4-dinitrophenyl acetate (DNPA). Enzymatic hydrolysis of DNPA was observed in the presence of the nanofibers by UV-vis spectroscopy and the Michaelis-Menten enzyme kinetics model. Significantly faster hydrolysis rate was observed in the presence of self-assembled nanofibers relative to spherical aggregates expected to have less order as well as single molecule catalysts.

AB - Catalytic particles based on supramolecular systems have demonstrated significant improvement in the field of catalysis. In this work, histidine-functionalized self-assembling peptide amphiphiles (PAs) were synthesized in order to form self-assembly high-aspect-ratio nanofibers with internal order that can present imidazolyl groups capable of ester hydrolysis. Self-assembly of the molecules making up the catalytic particle was studied by transmission electron microscopy and circular dichroism. The reactive sites were designed with histidine residues to catalyze the hydrolysis of 2,4-dinitrophenyl acetate (DNPA). Enzymatic hydrolysis of DNPA was observed in the presence of the nanofibers by UV-vis spectroscopy and the Michaelis-Menten enzyme kinetics model. Significantly faster hydrolysis rate was observed in the presence of self-assembled nanofibers relative to spherical aggregates expected to have less order as well as single molecule catalysts.

UR - http://www.scopus.com/inward/record.url?scp=35048837997&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35048837997&partnerID=8YFLogxK

U2 - 10.1021/ja075044n

DO - 10.1021/ja075044n

M3 - Article

VL - 129

SP - 12082

EP - 12083

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 40

ER -