A structural model for the charge separated state P700 ̇+A1 ̇+ photosystem I from the orientation of the magnetic interaction tensors

Stephan G. Zech, Wulf Hofbauer, Andreas Kamlowski, Petra Fromme, Dietmar Stehlik, Wolfgang Lubitz, Robert Bittl

Research output: Contribution to journalArticle

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Abstract

The charge separated state P700 ̇+A1 ̇+ (P700 = primary electron donor, A1 = phylloquinone electron acceptor) in photosystem I of oxygenic photosynthesis has been investigated by EPR spectroscopy in frozen solution and single crystals. The transient EPR spectra of P700 ̇+A1 ̇+ recorded in frozen solution of fully deuterated samples at X-, Q-, and W-band frequencies are shown to contain sufficient information to yield the orientation of the g-tensors of both A1 ̇+ and P700 ̇+ with respect to the axis connecting both spins. So far incomplete information on the orientation of A1 ̇+ relative to the membrane plane has been complemented by data from time-resolved EPR on single crystals measured at Q-band. The phylloquinone headgroup orientation evaluated from the EPR data in the charge-separated state P700 ̇+A1 ̇+ is compared with the presently available X-ray structural model. The g-tensor of P700 ̇+ has also been determined from cw-EPR experiments at W-band on single crystals, independent of the orientational data of the P700 ̇+ g-tensor from the time-resolved EPR experiments. The direction of the principal axes of g(P700 ̇+) differ from the molecular axes system of the chlorophylls comprising P700 as found previously in the case of P865 in bacterial reaction centers. The implications of the complete structural model from the A1 ̇+ and P700 ̇+ molecular magnetic interaction tensors in the active charge separated state P700 ̇+A1 ̇+ in PS I are discussed.

Original languageEnglish
Pages (from-to)9728-9739
Number of pages12
JournalJournal of Physical Chemistry B
Volume104
Issue number41
Publication statusPublished - Oct 19 2000

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Photosystem I Protein Complex
Tensors
Paramagnetic resonance
phylloquinone
tensors
Vitamin K 1
single crystals
Single crystals
interactions
photosynthesis
chlorophylls
electrons
Photosynthesis
Electrons
Chlorophyll
membranes
Frequency bands
Experiments
Spectroscopy
spectroscopy

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Engineering(all)

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A structural model for the charge separated state P700 ̇+A1 ̇+ photosystem I from the orientation of the magnetic interaction tensors. / Zech, Stephan G.; Hofbauer, Wulf; Kamlowski, Andreas; Fromme, Petra; Stehlik, Dietmar; Lubitz, Wolfgang; Bittl, Robert.

In: Journal of Physical Chemistry B, Vol. 104, No. 41, 19.10.2000, p. 9728-9739.

Research output: Contribution to journalArticle

Zech, Stephan G. ; Hofbauer, Wulf ; Kamlowski, Andreas ; Fromme, Petra ; Stehlik, Dietmar ; Lubitz, Wolfgang ; Bittl, Robert. / A structural model for the charge separated state P700 ̇+A1 ̇+ photosystem I from the orientation of the magnetic interaction tensors. In: Journal of Physical Chemistry B. 2000 ; Vol. 104, No. 41. pp. 9728-9739.
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abstract = "The charge separated state P700 ̇+A1 ̇+ (P700 = primary electron donor, A1 = phylloquinone electron acceptor) in photosystem I of oxygenic photosynthesis has been investigated by EPR spectroscopy in frozen solution and single crystals. The transient EPR spectra of P700 ̇+A1 ̇+ recorded in frozen solution of fully deuterated samples at X-, Q-, and W-band frequencies are shown to contain sufficient information to yield the orientation of the g-tensors of both A1 ̇+ and P700 ̇+ with respect to the axis connecting both spins. So far incomplete information on the orientation of A1 ̇+ relative to the membrane plane has been complemented by data from time-resolved EPR on single crystals measured at Q-band. The phylloquinone headgroup orientation evaluated from the EPR data in the charge-separated state P700 ̇+A1 ̇+ is compared with the presently available X-ray structural model. The g-tensor of P700 ̇+ has also been determined from cw-EPR experiments at W-band on single crystals, independent of the orientational data of the P700 ̇+ g-tensor from the time-resolved EPR experiments. The direction of the principal axes of g(P700 ̇+) differ from the molecular axes system of the chlorophylls comprising P700 as found previously in the case of P865 +̇ in bacterial reaction centers. The implications of the complete structural model from the A1 ̇+ and P700 ̇+ molecular magnetic interaction tensors in the active charge separated state P700 ̇+A1 ̇+ in PS I are discussed.",
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