A Three-Enzyme Pathway with an Optimised Geometric Arrangement to Facilitate Substrate Transfer

Minghui Liu, Jinglin Fu, Xiaodong Qi, Shaun Wootten, Neal W. Woodbury, Yan Liu, Hao Yan

Research output: Contribution to journalArticle

19 Citations (Scopus)


Cascade reactions drive and regulate a variety of metabolic activities. Efficient coupling of substrate transport between enzymes is important for overall pathway activity and also controls the depletion of intermediate molecules that drive the reaction forward. Here, we assembled a three-enzyme pathway on a series of DNA nanoscaffolds to investigate the dependence of their activities on spatial arrangement. Unlike previous studies, the overall activity of the three-enzyme pathway relied less on inter-enzyme distance and more on the geometric patterns that arranged them within a relatively small range of 10–30 nm. Pathway intermediate detection demonstrated that the assembled enzyme systems quickly depleted the intermediate molecules through efficient reaction coupling.

Original languageEnglish
Pages (from-to)1097-1101
Number of pages5
Publication statusPublished - Jun 16 2016



  • DNA nanostructures
  • enzyme cascade
  • malic dehydrogenase
  • self-assembly

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Molecular Medicine
  • Molecular Biology

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