Advances in cryogenic transmission electron microscopy for the characterization of dynamic self-assembling nanostructures

Christina J. Newcomb, Tyson J. Moyer, Sungsoo S. Lee, Samuel I Stupp

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Elucidating the structural information of nanoscale materials in their solvent-exposed state is crucial, as a result, cryogenic transmission electron microscopy (cryo-TEM) has become an increasingly popular technique in the materials science, chemistry, and biology communities. Cryo-TEM provides a method to directly visualize the specimen structure in a solution-state through a thin film of vitrified solvent. This technique complements X-ray, neutron, and light scattering methods that probe the statistical average of all species present; furthermore, cryo-TEM can be used to observe changes in structure over time. In the area of self-assembly, this tool has been particularly powerful for the characterization of natural and synthetic small molecule assemblies, as well as hybrid organic-inorganic composites. In this review, we discuss recent advances in cryogenic TEM in the context of self-assembling systems with emphasis on characterization of transitions observed in response to external stimuli.

Original languageEnglish
Pages (from-to)350-359
Number of pages10
JournalCurrent Opinion in Colloid and Interface Science
Volume17
Issue number6
DOIs
Publication statusPublished - Dec 2012

Fingerprint

assembling
Cryogenics
cryogenics
Nanostructures
Transmission electron microscopy
transmission electron microscopy
Materials science
materials science
Neutron scattering
X ray scattering
biology
Light scattering
complement
stimuli
Self assembly
assemblies
self assembly
neutron scattering
light scattering
chemistry

Keywords

  • Amyloid fibril assembly
  • Block copolymer assembly
  • Cryogenic transmission electron microscopy
  • Hybrid materials
  • Peptide amphiphile
  • Self-assembly

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Polymers and Plastics
  • Colloid and Surface Chemistry
  • Surfaces and Interfaces

Cite this

Advances in cryogenic transmission electron microscopy for the characterization of dynamic self-assembling nanostructures. / Newcomb, Christina J.; Moyer, Tyson J.; Lee, Sungsoo S.; Stupp, Samuel I.

In: Current Opinion in Colloid and Interface Science, Vol. 17, No. 6, 12.2012, p. 350-359.

Research output: Contribution to journalArticle

@article{92d52b8e87ce4f57b2553135421ff500,
title = "Advances in cryogenic transmission electron microscopy for the characterization of dynamic self-assembling nanostructures",
abstract = "Elucidating the structural information of nanoscale materials in their solvent-exposed state is crucial, as a result, cryogenic transmission electron microscopy (cryo-TEM) has become an increasingly popular technique in the materials science, chemistry, and biology communities. Cryo-TEM provides a method to directly visualize the specimen structure in a solution-state through a thin film of vitrified solvent. This technique complements X-ray, neutron, and light scattering methods that probe the statistical average of all species present; furthermore, cryo-TEM can be used to observe changes in structure over time. In the area of self-assembly, this tool has been particularly powerful for the characterization of natural and synthetic small molecule assemblies, as well as hybrid organic-inorganic composites. In this review, we discuss recent advances in cryogenic TEM in the context of self-assembling systems with emphasis on characterization of transitions observed in response to external stimuli.",
keywords = "Amyloid fibril assembly, Block copolymer assembly, Cryogenic transmission electron microscopy, Hybrid materials, Peptide amphiphile, Self-assembly",
author = "Newcomb, {Christina J.} and Moyer, {Tyson J.} and Lee, {Sungsoo S.} and Stupp, {Samuel I}",
year = "2012",
month = "12",
doi = "10.1016/j.cocis.2012.09.004",
language = "English",
volume = "17",
pages = "350--359",
journal = "Current Opinion in Colloid and Interface Science",
issn = "1359-0294",
publisher = "Elsevier BV",
number = "6",

}

TY - JOUR

T1 - Advances in cryogenic transmission electron microscopy for the characterization of dynamic self-assembling nanostructures

AU - Newcomb, Christina J.

AU - Moyer, Tyson J.

AU - Lee, Sungsoo S.

AU - Stupp, Samuel I

PY - 2012/12

Y1 - 2012/12

N2 - Elucidating the structural information of nanoscale materials in their solvent-exposed state is crucial, as a result, cryogenic transmission electron microscopy (cryo-TEM) has become an increasingly popular technique in the materials science, chemistry, and biology communities. Cryo-TEM provides a method to directly visualize the specimen structure in a solution-state through a thin film of vitrified solvent. This technique complements X-ray, neutron, and light scattering methods that probe the statistical average of all species present; furthermore, cryo-TEM can be used to observe changes in structure over time. In the area of self-assembly, this tool has been particularly powerful for the characterization of natural and synthetic small molecule assemblies, as well as hybrid organic-inorganic composites. In this review, we discuss recent advances in cryogenic TEM in the context of self-assembling systems with emphasis on characterization of transitions observed in response to external stimuli.

AB - Elucidating the structural information of nanoscale materials in their solvent-exposed state is crucial, as a result, cryogenic transmission electron microscopy (cryo-TEM) has become an increasingly popular technique in the materials science, chemistry, and biology communities. Cryo-TEM provides a method to directly visualize the specimen structure in a solution-state through a thin film of vitrified solvent. This technique complements X-ray, neutron, and light scattering methods that probe the statistical average of all species present; furthermore, cryo-TEM can be used to observe changes in structure over time. In the area of self-assembly, this tool has been particularly powerful for the characterization of natural and synthetic small molecule assemblies, as well as hybrid organic-inorganic composites. In this review, we discuss recent advances in cryogenic TEM in the context of self-assembling systems with emphasis on characterization of transitions observed in response to external stimuli.

KW - Amyloid fibril assembly

KW - Block copolymer assembly

KW - Cryogenic transmission electron microscopy

KW - Hybrid materials

KW - Peptide amphiphile

KW - Self-assembly

UR - http://www.scopus.com/inward/record.url?scp=84870672742&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870672742&partnerID=8YFLogxK

U2 - 10.1016/j.cocis.2012.09.004

DO - 10.1016/j.cocis.2012.09.004

M3 - Article

AN - SCOPUS:84870672742

VL - 17

SP - 350

EP - 359

JO - Current Opinion in Colloid and Interface Science

JF - Current Opinion in Colloid and Interface Science

SN - 1359-0294

IS - 6

ER -