Altered carbohydrate metabolism in glycogen synthase mutants of Synechococcus sp. strain PCC 7002: Cell factories for soluble sugars

Yu Xu, L. Tiago Guerra, Zhongkui Li, Marcus Ludwig, G Charles Dismukes, Donald A. Bryant

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Glycogen and compatible solutes are the major polymeric and soluble carbohydrates in cyanobacteria and function as energy reserves and osmoprotectants, respectively. Glycogen synthase null mutants (glgA-I glgA-II) were constructed in the cyanobacterium Synechococcus sp. strain PCC 7002. Under standard conditions the double mutant produced no glycogen and more soluble sugars. When grown under hypersaline conditions, the glgA-I glgA-II mutant accumulated 1.8-fold more soluble sugars (sucrose and glucosylglycer-(ol/ate)) than WT, and these cells spontaneously excreted soluble sugars into the medium at high levels without the need for additional transporters. An average of 27% more soluble sugars was released from the glgA-I glgA-II mutant than WT by hypo-osmotic shock. Extracellular vesicles budding from the outer membrane were observed by transmission electron microscopy in glgA-I glgA-II cells grown under hypersaline conditions. The glgA-I glgA-II mutant serves as a starting point for developing cell factories for photosynthetic production and excretion of sugars.

Original languageEnglish
Pages (from-to)56-67
Number of pages12
JournalMetabolic Engineering
Volume16
Issue number1
DOIs
Publication statusPublished - Mar 2013

Fingerprint

Synechococcus
Glycogen Synthase
Carbohydrate Metabolism
Sugars
Industrial plants
Cyanobacteria
Glycogen
Sugar (sucrose)
Osmotic Pressure
Transmission Electron Microscopy
Sucrose
Carbohydrates
Membranes
Cells
Transmission electron microscopy

Keywords

  • Autofermentation
  • Cyanobacteria
  • Glycogen
  • Hypersaline
  • Nitrogen limitation
  • Soluble sugars

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Altered carbohydrate metabolism in glycogen synthase mutants of Synechococcus sp. strain PCC 7002 : Cell factories for soluble sugars. / Xu, Yu; Tiago Guerra, L.; Li, Zhongkui; Ludwig, Marcus; Dismukes, G Charles; Bryant, Donald A.

In: Metabolic Engineering, Vol. 16, No. 1, 03.2013, p. 56-67.

Research output: Contribution to journalArticle

Xu, Yu ; Tiago Guerra, L. ; Li, Zhongkui ; Ludwig, Marcus ; Dismukes, G Charles ; Bryant, Donald A. / Altered carbohydrate metabolism in glycogen synthase mutants of Synechococcus sp. strain PCC 7002 : Cell factories for soluble sugars. In: Metabolic Engineering. 2013 ; Vol. 16, No. 1. pp. 56-67.
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