Abstract
A recent femtosecond X-ray diffraction study produced the first high-resolution structural model of the oxygen-evolving complex of photosystem II that is free of radiation-induced manganese reduction (Protein Data Bank entries 4UB6 and 4UB8). We find, however, that the model does not match extended X-ray absorption fine structure and QM/MM data for the S1 state. This is attributed to uncertainty about the positions of oxygen atoms that remain partially unresolved, even at 1.95 Å resolution, next to the heavy manganese centers. In addition, the photosystem II crystals may contain significant amounts of the S0 state, because of extensive dark adaptation prior to data collection.
Original language | English |
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Pages (from-to) | 1713-1716 |
Number of pages | 4 |
Journal | Biochemistry |
Volume | 54 |
Issue number | 9 |
DOIs | |
Publication status | Published - Mar 10 2015 |
ASJC Scopus subject areas
- Biochemistry