Abstract
A recent femtosecond X-ray diffraction study produced the first high-resolution structural model of the oxygen-evolving complex of photosystem II that is free of radiation-induced manganese reduction (Protein Data Bank entries 4UB6 and 4UB8). We find, however, that the model does not match extended X-ray absorption fine structure and QM/MM data for the S1 state. This is attributed to uncertainty about the positions of oxygen atoms that remain partially unresolved, even at 1.95 Å resolution, next to the heavy manganese centers. In addition, the photosystem II crystals may contain significant amounts of the S0 state, because of extensive dark adaptation prior to data collection.
| Original language | English |
|---|---|
| Pages (from-to) | 1713-1716 |
| Number of pages | 4 |
| Journal | Biochemistry |
| Volume | 54 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Mar 10 2015 |
Fingerprint
ASJC Scopus subject areas
- Biochemistry
- Medicine(all)
Cite this
Analysis of the radiation-damage-free x-ray structure of photosystem ii in light of exafs and QM/MM data. / Askerka, Mikhail; Vinyard, David J.; Wang, Jimin; Brudvig, Gary W; Batista, Victor S.
In: Biochemistry, Vol. 54, No. 9, 10.03.2015, p. 1713-1716.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analysis of the radiation-damage-free x-ray structure of photosystem ii in light of exafs and QM/MM data
AU - Askerka, Mikhail
AU - Vinyard, David J.
AU - Wang, Jimin
AU - Brudvig, Gary W
AU - Batista, Victor S.
PY - 2015/3/10
Y1 - 2015/3/10
N2 - A recent femtosecond X-ray diffraction study produced the first high-resolution structural model of the oxygen-evolving complex of photosystem II that is free of radiation-induced manganese reduction (Protein Data Bank entries 4UB6 and 4UB8). We find, however, that the model does not match extended X-ray absorption fine structure and QM/MM data for the S1 state. This is attributed to uncertainty about the positions of oxygen atoms that remain partially unresolved, even at 1.95 Å resolution, next to the heavy manganese centers. In addition, the photosystem II crystals may contain significant amounts of the S0 state, because of extensive dark adaptation prior to data collection.
AB - A recent femtosecond X-ray diffraction study produced the first high-resolution structural model of the oxygen-evolving complex of photosystem II that is free of radiation-induced manganese reduction (Protein Data Bank entries 4UB6 and 4UB8). We find, however, that the model does not match extended X-ray absorption fine structure and QM/MM data for the S1 state. This is attributed to uncertainty about the positions of oxygen atoms that remain partially unresolved, even at 1.95 Å resolution, next to the heavy manganese centers. In addition, the photosystem II crystals may contain significant amounts of the S0 state, because of extensive dark adaptation prior to data collection.
UR - http://www.scopus.com/inward/record.url?scp=84924346094&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84924346094&partnerID=8YFLogxK
U2 - 10.1021/acs.biochem.5b00089
DO - 10.1021/acs.biochem.5b00089
M3 - Article
VL - 54
SP - 1713
EP - 1716
JO - Biochemistry
JF - Biochemistry
SN - 0006-2960
IS - 9
ER -