Analysis of the radiation-damage-free x-ray structure of photosystem ii in light of exafs and QM/MM data

Mikhail Askerka, David J. Vinyard, Jimin Wang, Gary W Brudvig, Victor S. Batista

Research output: Contribution to journalArticle

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Abstract

A recent femtosecond X-ray diffraction study produced the first high-resolution structural model of the oxygen-evolving complex of photosystem II that is free of radiation-induced manganese reduction (Protein Data Bank entries 4UB6 and 4UB8). We find, however, that the model does not match extended X-ray absorption fine structure and QM/MM data for the S1 state. This is attributed to uncertainty about the positions of oxygen atoms that remain partially unresolved, even at 1.95 Å resolution, next to the heavy manganese centers. In addition, the photosystem II crystals may contain significant amounts of the S0 state, because of extensive dark adaptation prior to data collection.

Original languageEnglish
Pages (from-to)1713-1716
Number of pages4
JournalBiochemistry
Volume54
Issue number9
DOIs
Publication statusPublished - Mar 10 2015

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Photosystem II Protein Complex
Radiation damage
Manganese
X-Rays
Radiation
Oxygen
Dark Adaptation
X rays
Structural Models
X ray absorption
X-Ray Diffraction
Uncertainty
Databases
X ray diffraction
Atoms
Crystals
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)

Cite this

Analysis of the radiation-damage-free x-ray structure of photosystem ii in light of exafs and QM/MM data. / Askerka, Mikhail; Vinyard, David J.; Wang, Jimin; Brudvig, Gary W; Batista, Victor S.

In: Biochemistry, Vol. 54, No. 9, 10.03.2015, p. 1713-1716.

Research output: Contribution to journalArticle

Askerka, Mikhail ; Vinyard, David J. ; Wang, Jimin ; Brudvig, Gary W ; Batista, Victor S. / Analysis of the radiation-damage-free x-ray structure of photosystem ii in light of exafs and QM/MM data. In: Biochemistry. 2015 ; Vol. 54, No. 9. pp. 1713-1716.
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