Antitumor activity of peptide amphiphile nanofiber-encapsulated camptothecin

Stephen Soukasene, Daniel J. Toft, Tyson J. Moyer, Hsuming Lu, Hyung Kun Lee, Stephany M. Standley, Vincent L. Cryns, Samuel I Stupp

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

Self-assembling peptide amphiphile (PA) nanofibers were used to encapsulate camptothecin (CPT), a naturally occurring hydrophobic chemotherapy agent, using a solvent evaporation technique. Encapsulation by PA nanofibers was found to improve the aqueous solubility of the CPT molecule by more than 50-fold. PAs self-assembled into nanofibers in the presence of CPT as demonstrated by transmission electron microscopy. Small-angle X-ray scattering results suggest a slight increase in diameter of the nanofiber to accommodate the hydrophobic cargo. In vitro studies using human breast cancer cells show an enhancement in antitumor activity of the CPT when encapsulated by the PA nanofibers. In addition, using a mouse orthotopic model of human breast cancer, treatment with PA nanofiber-encapsulated CPT inhibited tumor growth. These results highlight the potential of this model PA system to be adapted for delivery of hydrophobic therapies to treat a variety of diseases including cancer.

Original languageEnglish
Pages (from-to)9113-9121
Number of pages9
JournalACS Nano
Volume5
Issue number11
DOIs
Publication statusPublished - Nov 22 2011

Fingerprint

Camptothecin
Amphiphiles
Nanofibers
Peptides
peptides
cancer
breast
cargo
chemotherapy
assembling
Oncology
Chemotherapy
mice
therapy
delivery
X ray scattering
Encapsulation
tumors
solubility
evaporation

Keywords

  • camptothecin
  • cancer therapy
  • drug delivery
  • nanofiber
  • peptide amphiphile
  • self-assembly

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

Soukasene, S., Toft, D. J., Moyer, T. J., Lu, H., Lee, H. K., Standley, S. M., ... Stupp, S. I. (2011). Antitumor activity of peptide amphiphile nanofiber-encapsulated camptothecin. ACS Nano, 5(11), 9113-9121. https://doi.org/10.1021/nn203343z

Antitumor activity of peptide amphiphile nanofiber-encapsulated camptothecin. / Soukasene, Stephen; Toft, Daniel J.; Moyer, Tyson J.; Lu, Hsuming; Lee, Hyung Kun; Standley, Stephany M.; Cryns, Vincent L.; Stupp, Samuel I.

In: ACS Nano, Vol. 5, No. 11, 22.11.2011, p. 9113-9121.

Research output: Contribution to journalArticle

Soukasene, S, Toft, DJ, Moyer, TJ, Lu, H, Lee, HK, Standley, SM, Cryns, VL & Stupp, SI 2011, 'Antitumor activity of peptide amphiphile nanofiber-encapsulated camptothecin', ACS Nano, vol. 5, no. 11, pp. 9113-9121. https://doi.org/10.1021/nn203343z
Soukasene S, Toft DJ, Moyer TJ, Lu H, Lee HK, Standley SM et al. Antitumor activity of peptide amphiphile nanofiber-encapsulated camptothecin. ACS Nano. 2011 Nov 22;5(11):9113-9121. https://doi.org/10.1021/nn203343z
Soukasene, Stephen ; Toft, Daniel J. ; Moyer, Tyson J. ; Lu, Hsuming ; Lee, Hyung Kun ; Standley, Stephany M. ; Cryns, Vincent L. ; Stupp, Samuel I. / Antitumor activity of peptide amphiphile nanofiber-encapsulated camptothecin. In: ACS Nano. 2011 ; Vol. 5, No. 11. pp. 9113-9121.
@article{c7450b93471d4ffba0ecbd968623a3c4,
title = "Antitumor activity of peptide amphiphile nanofiber-encapsulated camptothecin",
abstract = "Self-assembling peptide amphiphile (PA) nanofibers were used to encapsulate camptothecin (CPT), a naturally occurring hydrophobic chemotherapy agent, using a solvent evaporation technique. Encapsulation by PA nanofibers was found to improve the aqueous solubility of the CPT molecule by more than 50-fold. PAs self-assembled into nanofibers in the presence of CPT as demonstrated by transmission electron microscopy. Small-angle X-ray scattering results suggest a slight increase in diameter of the nanofiber to accommodate the hydrophobic cargo. In vitro studies using human breast cancer cells show an enhancement in antitumor activity of the CPT when encapsulated by the PA nanofibers. In addition, using a mouse orthotopic model of human breast cancer, treatment with PA nanofiber-encapsulated CPT inhibited tumor growth. These results highlight the potential of this model PA system to be adapted for delivery of hydrophobic therapies to treat a variety of diseases including cancer.",
keywords = "camptothecin, cancer therapy, drug delivery, nanofiber, peptide amphiphile, self-assembly",
author = "Stephen Soukasene and Toft, {Daniel J.} and Moyer, {Tyson J.} and Hsuming Lu and Lee, {Hyung Kun} and Standley, {Stephany M.} and Cryns, {Vincent L.} and Stupp, {Samuel I}",
year = "2011",
month = "11",
day = "22",
doi = "10.1021/nn203343z",
language = "English",
volume = "5",
pages = "9113--9121",
journal = "ACS Nano",
issn = "1936-0851",
publisher = "American Chemical Society",
number = "11",

}

TY - JOUR

T1 - Antitumor activity of peptide amphiphile nanofiber-encapsulated camptothecin

AU - Soukasene, Stephen

AU - Toft, Daniel J.

AU - Moyer, Tyson J.

AU - Lu, Hsuming

AU - Lee, Hyung Kun

AU - Standley, Stephany M.

AU - Cryns, Vincent L.

AU - Stupp, Samuel I

PY - 2011/11/22

Y1 - 2011/11/22

N2 - Self-assembling peptide amphiphile (PA) nanofibers were used to encapsulate camptothecin (CPT), a naturally occurring hydrophobic chemotherapy agent, using a solvent evaporation technique. Encapsulation by PA nanofibers was found to improve the aqueous solubility of the CPT molecule by more than 50-fold. PAs self-assembled into nanofibers in the presence of CPT as demonstrated by transmission electron microscopy. Small-angle X-ray scattering results suggest a slight increase in diameter of the nanofiber to accommodate the hydrophobic cargo. In vitro studies using human breast cancer cells show an enhancement in antitumor activity of the CPT when encapsulated by the PA nanofibers. In addition, using a mouse orthotopic model of human breast cancer, treatment with PA nanofiber-encapsulated CPT inhibited tumor growth. These results highlight the potential of this model PA system to be adapted for delivery of hydrophobic therapies to treat a variety of diseases including cancer.

AB - Self-assembling peptide amphiphile (PA) nanofibers were used to encapsulate camptothecin (CPT), a naturally occurring hydrophobic chemotherapy agent, using a solvent evaporation technique. Encapsulation by PA nanofibers was found to improve the aqueous solubility of the CPT molecule by more than 50-fold. PAs self-assembled into nanofibers in the presence of CPT as demonstrated by transmission electron microscopy. Small-angle X-ray scattering results suggest a slight increase in diameter of the nanofiber to accommodate the hydrophobic cargo. In vitro studies using human breast cancer cells show an enhancement in antitumor activity of the CPT when encapsulated by the PA nanofibers. In addition, using a mouse orthotopic model of human breast cancer, treatment with PA nanofiber-encapsulated CPT inhibited tumor growth. These results highlight the potential of this model PA system to be adapted for delivery of hydrophobic therapies to treat a variety of diseases including cancer.

KW - camptothecin

KW - cancer therapy

KW - drug delivery

KW - nanofiber

KW - peptide amphiphile

KW - self-assembly

UR - http://www.scopus.com/inward/record.url?scp=81855169692&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=81855169692&partnerID=8YFLogxK

U2 - 10.1021/nn203343z

DO - 10.1021/nn203343z

M3 - Article

VL - 5

SP - 9113

EP - 9121

JO - ACS Nano

JF - ACS Nano

SN - 1936-0851

IS - 11

ER -