Antitumor activity of peptide amphiphile nanofiber-encapsulated camptothecin

Stephen Soukasene, Daniel J. Toft, Tyson J. Moyer, Hsuming Lu, Hyung Kun Lee, Stephany M. Standley, Vincent L. Cryns, Samuel I. Stupp

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

Self-assembling peptide amphiphile (PA) nanofibers were used to encapsulate camptothecin (CPT), a naturally occurring hydrophobic chemotherapy agent, using a solvent evaporation technique. Encapsulation by PA nanofibers was found to improve the aqueous solubility of the CPT molecule by more than 50-fold. PAs self-assembled into nanofibers in the presence of CPT as demonstrated by transmission electron microscopy. Small-angle X-ray scattering results suggest a slight increase in diameter of the nanofiber to accommodate the hydrophobic cargo. In vitro studies using human breast cancer cells show an enhancement in antitumor activity of the CPT when encapsulated by the PA nanofibers. In addition, using a mouse orthotopic model of human breast cancer, treatment with PA nanofiber-encapsulated CPT inhibited tumor growth. These results highlight the potential of this model PA system to be adapted for delivery of hydrophobic therapies to treat a variety of diseases including cancer.

Original languageEnglish
Pages (from-to)9113-9121
Number of pages9
JournalACS nano
Volume5
Issue number11
DOIs
Publication statusPublished - Nov 22 2011

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Keywords

  • camptothecin
  • cancer therapy
  • drug delivery
  • nanofiber
  • peptide amphiphile
  • self-assembly

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Soukasene, S., Toft, D. J., Moyer, T. J., Lu, H., Lee, H. K., Standley, S. M., Cryns, V. L., & Stupp, S. I. (2011). Antitumor activity of peptide amphiphile nanofiber-encapsulated camptothecin. ACS nano, 5(11), 9113-9121. https://doi.org/10.1021/nn203343z