Arbitrary two-dimensional multiphoton excitation patterns with temporally focused digital holograms

Dan Oron, Eirini Papagiakomou, Vincent De-Sars, Valentina Emiliani

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Multiphoton excitation has recently found application in the fields of bioimaging, uncaging and lithography. In order to fully exploit the advantages of nonlinear excitation, in particular the axial resolution due to nonlinearity, most systems to date operate with point or multipoint excitation, while scanning either the laser beam or the sample to generate the illumination pattern. Here we combine the recently introduced technique of scanningless multiphoton excitation by temporal focusing with recent advances in digital holography to generate arbitrarily shaped, depth resolved, two-dimensional excitation patterns completely without scanning. This is of particular importance in applications requiring uniform excitation of large areas over short time scales, such as neuronal activation by multiphoton uncaging of neurotransmitters. We present an experimental and theoretical analysis of the effect of spatial patterning on the depth resolution achieved in temporal focusing microscopy. It is shown that the depth resolution for holographic excitation is somewhat worse than that achieved for uniform illumination. This is also accompanied by the appearance of a speckle pattern at the temporal focal plane. The origin of the two effects, as well as means to overcome them, are discussed.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7184
DOIs
Publication statusPublished - 2009
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI - San Jose, CA, United States
Duration: Jan 26 2009Jan 29 2009

Other

OtherThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI
CountryUnited States
CitySan Jose, CA
Period1/26/091/29/09

Fingerprint

Optical resolving power
Holograms
Lighting
Scanning
Holography
Speckle
Lithography
excitation
Laser beams
Neurotransmitter Agents
Microscopic examination
Chemical activation
Spatial Analysis
Microscopy
illumination
Lasers
neurotransmitters
scanning
speckle patterns
holography

Keywords

  • Digital holography
  • Multiphoton microscopy
  • Temporal focusing

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Oron, D., Papagiakomou, E., De-Sars, V., & Emiliani, V. (2009). Arbitrary two-dimensional multiphoton excitation patterns with temporally focused digital holograms. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7184). [71840S] https://doi.org/10.1117/12.808489

Arbitrary two-dimensional multiphoton excitation patterns with temporally focused digital holograms. / Oron, Dan; Papagiakomou, Eirini; De-Sars, Vincent; Emiliani, Valentina.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7184 2009. 71840S.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Oron, D, Papagiakomou, E, De-Sars, V & Emiliani, V 2009, Arbitrary two-dimensional multiphoton excitation patterns with temporally focused digital holograms. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7184, 71840S, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI, San Jose, CA, United States, 1/26/09. https://doi.org/10.1117/12.808489
Oron D, Papagiakomou E, De-Sars V, Emiliani V. Arbitrary two-dimensional multiphoton excitation patterns with temporally focused digital holograms. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7184. 2009. 71840S https://doi.org/10.1117/12.808489
Oron, Dan ; Papagiakomou, Eirini ; De-Sars, Vincent ; Emiliani, Valentina. / Arbitrary two-dimensional multiphoton excitation patterns with temporally focused digital holograms. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7184 2009.
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