Assembly of the tetra-Mn site of photosynthetic water oxidation by photoactivation: Mn stoichiometry and detection of a new intermediate

Gennady M. Ananyev, G. Charles Dismukes

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The process of photoactivation, the assembly of the water-oxidizing complex (WOC) of photosystem II (PSII) membranes, has been examined using two major improvements in methodology. First, a new lipophilic chelator, N,N,N',N'-tetrapropionato-1,3-bis(aminomethyl)benzene (TPDBA), has been used that permits complete extraction of both manganese and calcium and the three extrinsic WOC polypeptides while minimizing damage to the apo-PSII protein and, importantly, eliminating the need to use reductants. Second, an ultrasensitive, fast-response, polarographic cell and detection system were built. The apparatus features (a) an ultrabright red light-emitted diode (LED) for controlling the light intensity, pulse duration, and dark intervals, features critical for minimization of photoinhibition; (b) a microvolume (5 μL) O2 polarographic cell (Clark type) fitted with a thin silicone membrane for rapid response (100 ms); and (c) DC/AC preamplifier integrated into the microcell and interfaced to a bandpass AC amplifier. The sensitivity enables detection of ~5 x 10-14 mol of O2 per flash at a signal to noise = 5/1. These improvements permit 100-fold lower Mn concentrations to be explored. Under optimum conditions, complete recovery of O2-evolving activity could be restored compared to that of PSII membranes depicted of the three extrinsic polypeptides (35% V(max) vs intact PSII). Titration of the photoactivation steady-state O2 yield, Y(ss), and the half- time for recovery, t(1/2), vs Mn concentration demonstrate that 4.0 Mn/P680 are cooperatively taken up at 95% restoration of Y(ss) and that 1.1-1.2 Mn atoms are involved in the rate-limiting photolytic step under steady-state conditions. Due to minimization of photoinhibition, this intermediate exhibits a single exponential recovery kinetic over the entire population of PSII centers. Mn atoms in excess of 4 Mn/P680 accelerate the rate of photoactivation but decrease the yield above 8-10 Mn/P680. Maxima in both Y(ss) and t(1/2) are observed at similar electrochemical potentials of the medium, 380 and 340 mV, respectively. We attribute this maximum to either elimination of a recombination reaction between the redox-active tyrosine- 161 of the D1 polypeptide (Y(Z)+) and an electron acceptor, possibly cytochrome b559, or stabilization of an intermediate in photoactivation. At low Mn2+ concentrations, a new pro-steady-state kinetic intermediate which binds fewer than 4 Mn atoms can be directly observed. This early kinetic phase has a rate that depends on Mn concentration and is independent of the electron acceptor identity and concentration.

Original languageEnglish
Pages (from-to)4102-4109
Number of pages8
JournalBiochemistry
Volume35
Issue number13
DOIs
Publication statusPublished - Apr 2 1996

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Photosystem II Protein Complex
Stoichiometry
Oxidation
Water
Membranes
Recovery
Atoms
Peptides
Kinetics
Electrons
Light
Reducing Agents
Silicones
Chelating Agents
Manganese
Titration
Genetic Recombination
Restoration
Oxidation-Reduction
Tyrosine

ASJC Scopus subject areas

  • Biochemistry

Cite this

Assembly of the tetra-Mn site of photosynthetic water oxidation by photoactivation : Mn stoichiometry and detection of a new intermediate. / Ananyev, Gennady M.; Dismukes, G. Charles.

In: Biochemistry, Vol. 35, No. 13, 02.04.1996, p. 4102-4109.

Research output: Contribution to journalArticle

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