A new method, that of "auto-reconvolution", for the recovery of rotational relaxation data from time-resolved anisotropy measurements (TRAMS) is reported. In this approach the observed time-dependent "parallel" component of luminescence intensity, I|(t), is used to deconvolute its orthogonal counterpart, I⊥(t). The procedure has been validated in TRAMS experiments upon four fluorescently labelled polymers in dilute solution, following vertically polarized excitation using synchrotron radiation. In each case, excellent agreement with data retrieved by the impulse reconvolution method was achieved.
ASJC Scopus subject areas
- Physics and Astronomy(all)
- Physical and Theoretical Chemistry