Binding of metal ions to melanin and their effects on the aerobic reactivity

Lian Hong, Yan Liu, John D. Simon

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The binding of Mg(II), Ca(II), Zn(II), Cu(II) and Fe(III) to ETDA-washed Sepia melanin is quantified by inductively coupled plasma mass spectrometry. By monitoring the solution pH change associated with metal uptake, it is concluded that Mg(II), Ca(II) and Zn(II) bind to carboxylic acid groups in melanin, Cu(II) binds to hydroxyl (OH) groups and Fe(III) binds to OH or amine groups. The aerobic reactivity of melanins with different metal contents is analyzed by examining their ability to cause strand breaks in supercoiled pUC18 DNA. Cu(II)- and Fe(III)-enriched melanins induce the most damage. Hydroxyl radical, OH, is proposed to be one of the reactive oxygen species responsible.

Original languageEnglish
Pages (from-to)477-481
Number of pages5
JournalPhotochemistry and Photobiology
Volume80
Issue number3
DOIs
Publication statusPublished - Nov 2004

Fingerprint

melanin
Melanins
Metal ions
metal ions
reactivity
Metals
Ions
Hydroxyl Radical
Sepia
Superhelical DNA
Inductively coupled plasma mass spectrometry
inductively coupled plasma mass spectrometry
hydroxyl radicals
Carboxylic Acids
carboxylic acids
strands
metals
Amines
Reactive Oxygen Species
Mass Spectrometry

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Binding of metal ions to melanin and their effects on the aerobic reactivity. / Hong, Lian; Liu, Yan; Simon, John D.

In: Photochemistry and Photobiology, Vol. 80, No. 3, 11.2004, p. 477-481.

Research output: Contribution to journalArticle

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