Bioactive DNA-peptide nanotubes enhance the differentiation of neural stem cells into neurons

Nicholas Stephanopoulos, Ronit Freeman, Hilary A. North, Shantanu Sur, Su Ji Jeong, Faifan Tantakitti, John A. Kessler, Samuel I Stupp

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

We report the construction of DNA nanotubes covalently functionalized with the cell adhesion peptide RGDS as a bioactive substrate for neural stem cell differentiation. Alteration of the Watson-Crick base pairing program that builds the nanostructures allowed us to probe independently the effect of nanotube architecture and peptide bioactivity on stem cell differentiation. We found that both factors instruct synergistically the preferential differentiation of the cells into neurons rather than astrocytes.

Original languageEnglish
Pages (from-to)603-609
Number of pages7
JournalNano Letters
Volume15
Issue number1
DOIs
Publication statusPublished - Jan 14 2015

Fingerprint

Peptide Nanotubes
stem cells
activity (biology)
Stem cells
neurons
Nanotubes
Peptides
Neurons
peptides
nanotubes
arginyl-glycyl-aspartyl-serine
DNA
deoxyribonucleic acid
Cell adhesion
Bioactivity
Nanostructures
Cells
cells
adhesion
Substrates

Keywords

  • biomaterials
  • DNA nanotechnology
  • DNA-peptide
  • extracellular matrix
  • neural stem cells
  • Self-assembly

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanical Engineering

Cite this

Stephanopoulos, N., Freeman, R., North, H. A., Sur, S., Jeong, S. J., Tantakitti, F., ... Stupp, S. I. (2015). Bioactive DNA-peptide nanotubes enhance the differentiation of neural stem cells into neurons. Nano Letters, 15(1), 603-609. https://doi.org/10.1021/nl504079q

Bioactive DNA-peptide nanotubes enhance the differentiation of neural stem cells into neurons. / Stephanopoulos, Nicholas; Freeman, Ronit; North, Hilary A.; Sur, Shantanu; Jeong, Su Ji; Tantakitti, Faifan; Kessler, John A.; Stupp, Samuel I.

In: Nano Letters, Vol. 15, No. 1, 14.01.2015, p. 603-609.

Research output: Contribution to journalArticle

Stephanopoulos, N, Freeman, R, North, HA, Sur, S, Jeong, SJ, Tantakitti, F, Kessler, JA & Stupp, SI 2015, 'Bioactive DNA-peptide nanotubes enhance the differentiation of neural stem cells into neurons', Nano Letters, vol. 15, no. 1, pp. 603-609. https://doi.org/10.1021/nl504079q
Stephanopoulos N, Freeman R, North HA, Sur S, Jeong SJ, Tantakitti F et al. Bioactive DNA-peptide nanotubes enhance the differentiation of neural stem cells into neurons. Nano Letters. 2015 Jan 14;15(1):603-609. https://doi.org/10.1021/nl504079q
Stephanopoulos, Nicholas ; Freeman, Ronit ; North, Hilary A. ; Sur, Shantanu ; Jeong, Su Ji ; Tantakitti, Faifan ; Kessler, John A. ; Stupp, Samuel I. / Bioactive DNA-peptide nanotubes enhance the differentiation of neural stem cells into neurons. In: Nano Letters. 2015 ; Vol. 15, No. 1. pp. 603-609.
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