Bioactive Nanofibers Induce Neural Transdifferentiation of Human Bone Marrow Mesenchymal Stem Cells

Wei Ji, Zaida Álvarez, Alexandra N. Edelbrock, Kohei Sato, Samuel I Stupp

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The combination of biomaterials with stem cells is a promising therapeutic strategy to repair traumatic injuries in the central nervous system, and human bone marrow mesenchymal stem cells (BMSCs) offer a clinically translatable option among other possible sources of stem cells. We report here on the use of a supramolecular bioactive material based on a peptide amphiphile (PA), displaying a laminin-mimetic IKVAV sequence to drive neural transdifferentiation of human BMSCs. The IKVAV-PA self-assembles into supramolecular nanofibers that induce neuroectodermal lineage commitment after 1 week, as evidenced by the upregulation of the neural progenitor gene nestin (NES) and glial fibrillary acidic protein (GFAP). After 2 weeks, the bioactive IKVAV-PA nanofibers induce significantly higher expression of neuronal markers β-III tubulin (TUJ-1), microtubule-associated protein-2 (MAP-2), and neuronal nuclei (NEUN), as well as the extracellular matrix laminin (LMN). Furthermore, the human BMSCs exposed to the biomaterial reveal a polarized cytoskeletal architecture and a decrease in cellular size, resembling neuron-like cells. We conclude that the investigated supramolecular biomaterial opens the opportunity to transdifferentiate adult human BMSCs into neuronal lineage.

Original languageEnglish
Pages (from-to)41046-41055
Number of pages10
JournalACS Applied Materials and Interfaces
Volume10
Issue number48
DOIs
Publication statusPublished - Dec 5 2018

Fingerprint

isoleucyl-lysyl-valyl-alanyl-valine
Nanofibers
Stem cells
Bone
Amphiphiles
Biocompatible Materials
Biomaterials
Peptides
Laminin
Proteins
Nestin
Microtubule-Associated Proteins
Glial Fibrillary Acidic Protein
Neurology
Tubulin
Neurons
Repair
Genes

Keywords

  • human mesenchymal stem cell
  • IKVAV
  • neurons
  • peptide amphiphile
  • transdifferentiation

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Bioactive Nanofibers Induce Neural Transdifferentiation of Human Bone Marrow Mesenchymal Stem Cells. / Ji, Wei; Álvarez, Zaida; Edelbrock, Alexandra N.; Sato, Kohei; Stupp, Samuel I.

In: ACS Applied Materials and Interfaces, Vol. 10, No. 48, 05.12.2018, p. 41046-41055.

Research output: Contribution to journalArticle

Ji, Wei ; Álvarez, Zaida ; Edelbrock, Alexandra N. ; Sato, Kohei ; Stupp, Samuel I. / Bioactive Nanofibers Induce Neural Transdifferentiation of Human Bone Marrow Mesenchymal Stem Cells. In: ACS Applied Materials and Interfaces. 2018 ; Vol. 10, No. 48. pp. 41046-41055.
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