Bioactive peptide amphiphile nanofiber gels enhance burn wound healing

Situo Zhou, Akishige Hokugo, Mark McClendon, Zheyu Zhang, Reena Bakshi, Lixin Wang, Luis Andres Segovia, Kameron Rezzadeh, Samuel I Stupp, Reza Jarrahy

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Burns are physically debilitating and potentially fatal injuries. The standard-of-care for burn wounds is the coverage with gauze dressings designed to minimize trauma to the regenerating epidermis and dermis during dressing changes. However, deep partial- and full-thickness burns always heal slowly when standard wound care alone is performed. We have previously reported that peptide amphiphile (PA)gels, pH-induced self-assembling nanostructured fibrous scaffolds, promote cell proliferation and have great potential in regenerative medicine for rapid repair of tissues. In this study, we hypothesized that the PA gels are capable of accelerating wound healing in burn injury. Methods: Artificially generated thermally damaged fibroblasts and human umbilical vein endothelial cells were seeded onto the various PA nanofiber gels including bioactive and nonbioactive peptide sequences. Cell proliferation was assessed at different time points, and thermally damaged fibroblasts and HUVECs manifested increased proliferation with time when cultured with various PA gels. To determine in vivo effects, burn wounds of rats were treated with the bioactive Arg-Gly-Asp-Ser (RGDS)-modified gel that showed greater cell proliferation in vitro. The wound closure was observed, and skin samples were harvested for histologic evaluation. Results: Cell proliferation using the RGDS-PA gel was significantly higher than that observed in other gels. The RGDS-PA gel significantly enhanced re-epithelialization during the burn wound healing process between days 7 and 28. Application of PA gels accelerates the recovery of deep partial-thickness burn wounds by stimulation of fibroblasts and the creation of an environment conducive to epithelial cell proliferation and wound closure. Conclusions: This biomaterial represents a new therapeutic strategy to overcome current clinical challenges in the treatment of injuries resulting from burns.

Original languageEnglish
JournalBurns
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Nanofibers
Wound Healing
Gels
Peptides
Wounds and Injuries
arginyl-glycyl-aspartyl-serine
Cell Proliferation
Burns
Fibroblasts
Bandages
Re-Epithelialization
Regenerative Medicine
Human Umbilical Vein Endothelial Cells
Biocompatible Materials
Standard of Care
Dermis
Epidermis
Epithelial Cells

Keywords

  • Nanofiber
  • Peptide amphiphile
  • Self-assembly
  • Thermally damaged cells
  • Wound healing

ASJC Scopus subject areas

  • Surgery
  • Emergency Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Zhou, S., Hokugo, A., McClendon, M., Zhang, Z., Bakshi, R., Wang, L., ... Jarrahy, R. (2019). Bioactive peptide amphiphile nanofiber gels enhance burn wound healing. Burns. https://doi.org/10.1016/j.burns.2018.06.008

Bioactive peptide amphiphile nanofiber gels enhance burn wound healing. / Zhou, Situo; Hokugo, Akishige; McClendon, Mark; Zhang, Zheyu; Bakshi, Reena; Wang, Lixin; Segovia, Luis Andres; Rezzadeh, Kameron; Stupp, Samuel I; Jarrahy, Reza.

In: Burns, 01.01.2019.

Research output: Contribution to journalArticle

Zhou, S, Hokugo, A, McClendon, M, Zhang, Z, Bakshi, R, Wang, L, Segovia, LA, Rezzadeh, K, Stupp, SI & Jarrahy, R 2019, 'Bioactive peptide amphiphile nanofiber gels enhance burn wound healing', Burns. https://doi.org/10.1016/j.burns.2018.06.008
Zhou S, Hokugo A, McClendon M, Zhang Z, Bakshi R, Wang L et al. Bioactive peptide amphiphile nanofiber gels enhance burn wound healing. Burns. 2019 Jan 1. https://doi.org/10.1016/j.burns.2018.06.008
Zhou, Situo ; Hokugo, Akishige ; McClendon, Mark ; Zhang, Zheyu ; Bakshi, Reena ; Wang, Lixin ; Segovia, Luis Andres ; Rezzadeh, Kameron ; Stupp, Samuel I ; Jarrahy, Reza. / Bioactive peptide amphiphile nanofiber gels enhance burn wound healing. In: Burns. 2019.
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abstract = "Background: Burns are physically debilitating and potentially fatal injuries. The standard-of-care for burn wounds is the coverage with gauze dressings designed to minimize trauma to the regenerating epidermis and dermis during dressing changes. However, deep partial- and full-thickness burns always heal slowly when standard wound care alone is performed. We have previously reported that peptide amphiphile (PA)gels, pH-induced self-assembling nanostructured fibrous scaffolds, promote cell proliferation and have great potential in regenerative medicine for rapid repair of tissues. In this study, we hypothesized that the PA gels are capable of accelerating wound healing in burn injury. Methods: Artificially generated thermally damaged fibroblasts and human umbilical vein endothelial cells were seeded onto the various PA nanofiber gels including bioactive and nonbioactive peptide sequences. Cell proliferation was assessed at different time points, and thermally damaged fibroblasts and HUVECs manifested increased proliferation with time when cultured with various PA gels. To determine in vivo effects, burn wounds of rats were treated with the bioactive Arg-Gly-Asp-Ser (RGDS)-modified gel that showed greater cell proliferation in vitro. The wound closure was observed, and skin samples were harvested for histologic evaluation. Results: Cell proliferation using the RGDS-PA gel was significantly higher than that observed in other gels. The RGDS-PA gel significantly enhanced re-epithelialization during the burn wound healing process between days 7 and 28. Application of PA gels accelerates the recovery of deep partial-thickness burn wounds by stimulation of fibroblasts and the creation of an environment conducive to epithelial cell proliferation and wound closure. Conclusions: This biomaterial represents a new therapeutic strategy to overcome current clinical challenges in the treatment of injuries resulting from burns.",
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AU - Zhou, Situo

AU - Hokugo, Akishige

AU - McClendon, Mark

AU - Zhang, Zheyu

AU - Bakshi, Reena

AU - Wang, Lixin

AU - Segovia, Luis Andres

AU - Rezzadeh, Kameron

AU - Stupp, Samuel I

AU - Jarrahy, Reza

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N2 - Background: Burns are physically debilitating and potentially fatal injuries. The standard-of-care for burn wounds is the coverage with gauze dressings designed to minimize trauma to the regenerating epidermis and dermis during dressing changes. However, deep partial- and full-thickness burns always heal slowly when standard wound care alone is performed. We have previously reported that peptide amphiphile (PA)gels, pH-induced self-assembling nanostructured fibrous scaffolds, promote cell proliferation and have great potential in regenerative medicine for rapid repair of tissues. In this study, we hypothesized that the PA gels are capable of accelerating wound healing in burn injury. Methods: Artificially generated thermally damaged fibroblasts and human umbilical vein endothelial cells were seeded onto the various PA nanofiber gels including bioactive and nonbioactive peptide sequences. Cell proliferation was assessed at different time points, and thermally damaged fibroblasts and HUVECs manifested increased proliferation with time when cultured with various PA gels. To determine in vivo effects, burn wounds of rats were treated with the bioactive Arg-Gly-Asp-Ser (RGDS)-modified gel that showed greater cell proliferation in vitro. The wound closure was observed, and skin samples were harvested for histologic evaluation. Results: Cell proliferation using the RGDS-PA gel was significantly higher than that observed in other gels. The RGDS-PA gel significantly enhanced re-epithelialization during the burn wound healing process between days 7 and 28. Application of PA gels accelerates the recovery of deep partial-thickness burn wounds by stimulation of fibroblasts and the creation of an environment conducive to epithelial cell proliferation and wound closure. Conclusions: This biomaterial represents a new therapeutic strategy to overcome current clinical challenges in the treatment of injuries resulting from burns.

AB - Background: Burns are physically debilitating and potentially fatal injuries. The standard-of-care for burn wounds is the coverage with gauze dressings designed to minimize trauma to the regenerating epidermis and dermis during dressing changes. However, deep partial- and full-thickness burns always heal slowly when standard wound care alone is performed. We have previously reported that peptide amphiphile (PA)gels, pH-induced self-assembling nanostructured fibrous scaffolds, promote cell proliferation and have great potential in regenerative medicine for rapid repair of tissues. In this study, we hypothesized that the PA gels are capable of accelerating wound healing in burn injury. Methods: Artificially generated thermally damaged fibroblasts and human umbilical vein endothelial cells were seeded onto the various PA nanofiber gels including bioactive and nonbioactive peptide sequences. Cell proliferation was assessed at different time points, and thermally damaged fibroblasts and HUVECs manifested increased proliferation with time when cultured with various PA gels. To determine in vivo effects, burn wounds of rats were treated with the bioactive Arg-Gly-Asp-Ser (RGDS)-modified gel that showed greater cell proliferation in vitro. The wound closure was observed, and skin samples were harvested for histologic evaluation. Results: Cell proliferation using the RGDS-PA gel was significantly higher than that observed in other gels. The RGDS-PA gel significantly enhanced re-epithelialization during the burn wound healing process between days 7 and 28. Application of PA gels accelerates the recovery of deep partial-thickness burn wounds by stimulation of fibroblasts and the creation of an environment conducive to epithelial cell proliferation and wound closure. Conclusions: This biomaterial represents a new therapeutic strategy to overcome current clinical challenges in the treatment of injuries resulting from burns.

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