Biocompatibility of calcined mesoporous silica particles with ventricular myocyte structure and function

Elhadi H. Aburawi, Mohammed Anwar Qureshi, Deniz Oz, Petrilla Jayaprakash, Saeed Tariq, Rashed S. Hameed, Sayantani Das, Anandarup Goswami, Ankush V. Biradar, Teddy Asefa, Abdul Kader Souid, Ernest Adeghate, Frank Christopher Howarth

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Abstract

In vivo and in vitro systems were employed to investigate the biocompatibility of two forms of calcined mesoporous silica microparticles, MCM41-cal and SBA15-cal, with ventricular myocytes. These particles have potential clinical use in delivering bioactive compounds to the heart. Ventricular myocytes were isolated from 6 to 8 week male Wistar rats. The distribution of the particles in ventricular myocytes was investigated by transmission electron microscopy and scanning electron microscopy. The distribution of particles was also examined in cardiac muscle 10 min after intravenous injection of 2.0 mg/mL MCM41-cal. Myocyte shortening and the Ca 2+ transient were determined following exposure to 200 μg/mL MCM41-cal or SBA15-cal for 10 min. Within 10 min of incubation at 25 C, both MCM41-cal and SBA15-cal were found attached to the plasma membrane, and some particles were observed inside ventricular myocytes. MCM41-cal was more abundant inside the myocytes than SBA15-cal. The particles had a notable affinity to mitochondrial membranes, where they eventually settled. Within 10 min of intravenous injection (2.0 mg/mL), MCM41-cal traversed the perivascular space, and some particles entered ventricular myocytes and localized around the mitochondrial membranes. The amplitude of shortening was slightly reduced in myocytes superperfused with MCM41-cal or SBA15-cal. The amplitude of the Ca 2+ transient was significantly reduced in myocytes superperfused with MCM41-cal but was only slightly reduced with SBA15-cal. Overall, the results show reasonable bioavailability and biocompatibility of MCM41-cal and SBA15-cal with ventricular myocytes.

Original languageEnglish
Pages (from-to)26-36
Number of pages11
JournalChemical Research in Toxicology
Volume26
Issue number1
DOIs
Publication statusPublished - Jan 18 2013

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Biocompatibility
Silicon Dioxide
Muscle Cells
Membranes
Cell membranes
Muscle
Rats
Transmission electron microscopy
Scanning electron microscopy
Mitochondrial Membranes
Intravenous Injections
Transmission Electron Microscopy
Electron Scanning Microscopy
Biological Availability
Wistar Rats
Myocardium
Cell Membrane

ASJC Scopus subject areas

  • Toxicology

Cite this

Aburawi, E. H., Qureshi, M. A., Oz, D., Jayaprakash, P., Tariq, S., Hameed, R. S., ... Howarth, F. C. (2013). Biocompatibility of calcined mesoporous silica particles with ventricular myocyte structure and function. Chemical Research in Toxicology, 26(1), 26-36. https://doi.org/10.1021/tx300255u

Biocompatibility of calcined mesoporous silica particles with ventricular myocyte structure and function. / Aburawi, Elhadi H.; Qureshi, Mohammed Anwar; Oz, Deniz; Jayaprakash, Petrilla; Tariq, Saeed; Hameed, Rashed S.; Das, Sayantani; Goswami, Anandarup; Biradar, Ankush V.; Asefa, Teddy; Souid, Abdul Kader; Adeghate, Ernest; Howarth, Frank Christopher.

In: Chemical Research in Toxicology, Vol. 26, No. 1, 18.01.2013, p. 26-36.

Research output: Contribution to journalArticle

Aburawi, EH, Qureshi, MA, Oz, D, Jayaprakash, P, Tariq, S, Hameed, RS, Das, S, Goswami, A, Biradar, AV, Asefa, T, Souid, AK, Adeghate, E & Howarth, FC 2013, 'Biocompatibility of calcined mesoporous silica particles with ventricular myocyte structure and function', Chemical Research in Toxicology, vol. 26, no. 1, pp. 26-36. https://doi.org/10.1021/tx300255u
Aburawi, Elhadi H. ; Qureshi, Mohammed Anwar ; Oz, Deniz ; Jayaprakash, Petrilla ; Tariq, Saeed ; Hameed, Rashed S. ; Das, Sayantani ; Goswami, Anandarup ; Biradar, Ankush V. ; Asefa, Teddy ; Souid, Abdul Kader ; Adeghate, Ernest ; Howarth, Frank Christopher. / Biocompatibility of calcined mesoporous silica particles with ventricular myocyte structure and function. In: Chemical Research in Toxicology. 2013 ; Vol. 26, No. 1. pp. 26-36.
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AU - Biradar, Ankush V.

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AU - Souid, Abdul Kader

AU - Adeghate, Ernest

AU - Howarth, Frank Christopher

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