Abstract
Inorganic manganese-based particles are becoming attractive for molecular and cellular imaging, due to their ability to provide bright contrast on MRI, as opposed to the dark contrast generated from iron-based particles. Using a single emulsion technique, we have successfully fabricated pH-sensitive poly(lactic-co-glycolic acid) (PLGA)-encapsulated manganese oxide (MnO) nanocrystals. Two classes of particles were fabricated at ∼140 nm and 1.7 μm and incorporated 15 to 20 nm MnO nanocrystals with high encapsulation efficiencies. Intact particles at physiological pH cause little contrast in MRI, but following endocytosis into low pH compartments within the cells, the particles erode and MnO dissolves to release Mn2+. This causes the cells to appear bright on MR images. The magnitude of the change in MRI properties is as high as 35-fold, making it the most dynamic "smart" MRI contrast agent yet reported. Possible applications of these MnO particles include slow release Mn2+, tumor targeting, and confirmation of cell uptake.
| Original language | English |
|---|---|
| Pages (from-to) | 3438-3446 |
| Number of pages | 9 |
| Journal | ACS Nano |
| Volume | 5 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - May 24 2011 |
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Keywords
- Cells
- Contrast agents
- Manganese
- Microparticles
- MRI
- Nanoparticles
- PLGA
ASJC Scopus subject areas
- Engineering(all)
- Materials Science(all)
- Physics and Astronomy(all)
Cite this
Biocompatible and pH-sensitive PLGA encapsulated MnO nanocrystals for molecular and cellular MRI. / Bennewitz, Margaret F.; Lobo, Tricia L.; Nkansah, Michael K.; Ulas, Gözde; Brudvig, Gary W; Shapiro, Erik M.
In: ACS Nano, Vol. 5, No. 5, 24.05.2011, p. 3438-3446.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biocompatible and pH-sensitive PLGA encapsulated MnO nanocrystals for molecular and cellular MRI
AU - Bennewitz, Margaret F.
AU - Lobo, Tricia L.
AU - Nkansah, Michael K.
AU - Ulas, Gözde
AU - Brudvig, Gary W
AU - Shapiro, Erik M.
PY - 2011/5/24
Y1 - 2011/5/24
N2 - Inorganic manganese-based particles are becoming attractive for molecular and cellular imaging, due to their ability to provide bright contrast on MRI, as opposed to the dark contrast generated from iron-based particles. Using a single emulsion technique, we have successfully fabricated pH-sensitive poly(lactic-co-glycolic acid) (PLGA)-encapsulated manganese oxide (MnO) nanocrystals. Two classes of particles were fabricated at ∼140 nm and 1.7 μm and incorporated 15 to 20 nm MnO nanocrystals with high encapsulation efficiencies. Intact particles at physiological pH cause little contrast in MRI, but following endocytosis into low pH compartments within the cells, the particles erode and MnO dissolves to release Mn2+. This causes the cells to appear bright on MR images. The magnitude of the change in MRI properties is as high as 35-fold, making it the most dynamic "smart" MRI contrast agent yet reported. Possible applications of these MnO particles include slow release Mn2+, tumor targeting, and confirmation of cell uptake.
AB - Inorganic manganese-based particles are becoming attractive for molecular and cellular imaging, due to their ability to provide bright contrast on MRI, as opposed to the dark contrast generated from iron-based particles. Using a single emulsion technique, we have successfully fabricated pH-sensitive poly(lactic-co-glycolic acid) (PLGA)-encapsulated manganese oxide (MnO) nanocrystals. Two classes of particles were fabricated at ∼140 nm and 1.7 μm and incorporated 15 to 20 nm MnO nanocrystals with high encapsulation efficiencies. Intact particles at physiological pH cause little contrast in MRI, but following endocytosis into low pH compartments within the cells, the particles erode and MnO dissolves to release Mn2+. This causes the cells to appear bright on MR images. The magnitude of the change in MRI properties is as high as 35-fold, making it the most dynamic "smart" MRI contrast agent yet reported. Possible applications of these MnO particles include slow release Mn2+, tumor targeting, and confirmation of cell uptake.
KW - Cells
KW - Contrast agents
KW - Manganese
KW - Microparticles
KW - MRI
KW - Nanoparticles
KW - PLGA
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U2 - 10.1021/nn1019779
DO - 10.1021/nn1019779
M3 - Article
VL - 5
SP - 3438
EP - 3446
JO - ACS Nano
JF - ACS Nano
SN - 1936-0851
IS - 5
ER -