Biological synthesis of tooth enamel instructed by an artificial matrix

Zhan Huang, Christina J. Newcomb, Pablo Bringas, Samuel I Stupp, Malcolm L. Snead

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The regenerative capability of enamel, the hardest tissue in the vertebrate body, is fundamentally limited due to cell apoptosis following maturation of the tissue. Synthetic strategies to promote enamel formation have the potential to repair damage, increase the longevity of teeth and improve the understanding of the events leading to tissue formation. Using a self-assembling bioactive matrix, we demonstrate the ability to induce ectopic formation of enamel at chosen sites adjacent to a mouse incisor cultured in vivo under the kidney capsule. The resulting material reveals the highly organized, hierarchical structure of hydroxyapatite crystallites similar to native enamel. This artificially triggered formation of organized mineral demonstrates a pathway for developing cell fabricated materials for treatment of dental caries, the most ubiquitous disease in man. Additionally, the artificial matrix provides a unique tool to probe cellular mechanisms involved in tissue formation further enabling the development of tooth organ replacements.

Original languageEnglish
Pages (from-to)9202-9211
Number of pages10
JournalBiomaterials
Volume31
Issue number35
DOIs
Publication statusPublished - Dec 2010

Fingerprint

Tooth enamel
Enamels
Dental Enamel
Tooth
Tissue
Dental Caries
Cell death
Incisor
Durapatite
Crystallites
Hydroxyapatite
Capsules
Minerals
Vertebrates
Repair
Apoptosis
Kidney

Keywords

  • Cell activation
  • Epithelial cell
  • Extracellular matrix
  • Peptide amphiphile
  • RGDS peptide
  • Self-assembly

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Biological synthesis of tooth enamel instructed by an artificial matrix. / Huang, Zhan; Newcomb, Christina J.; Bringas, Pablo; Stupp, Samuel I; Snead, Malcolm L.

In: Biomaterials, Vol. 31, No. 35, 12.2010, p. 9202-9211.

Research output: Contribution to journalArticle

Huang, Zhan ; Newcomb, Christina J. ; Bringas, Pablo ; Stupp, Samuel I ; Snead, Malcolm L. / Biological synthesis of tooth enamel instructed by an artificial matrix. In: Biomaterials. 2010 ; Vol. 31, No. 35. pp. 9202-9211.
@article{4b90163ea13642648bb2092dfbfcd608,
title = "Biological synthesis of tooth enamel instructed by an artificial matrix",
abstract = "The regenerative capability of enamel, the hardest tissue in the vertebrate body, is fundamentally limited due to cell apoptosis following maturation of the tissue. Synthetic strategies to promote enamel formation have the potential to repair damage, increase the longevity of teeth and improve the understanding of the events leading to tissue formation. Using a self-assembling bioactive matrix, we demonstrate the ability to induce ectopic formation of enamel at chosen sites adjacent to a mouse incisor cultured in vivo under the kidney capsule. The resulting material reveals the highly organized, hierarchical structure of hydroxyapatite crystallites similar to native enamel. This artificially triggered formation of organized mineral demonstrates a pathway for developing cell fabricated materials for treatment of dental caries, the most ubiquitous disease in man. Additionally, the artificial matrix provides a unique tool to probe cellular mechanisms involved in tissue formation further enabling the development of tooth organ replacements.",
keywords = "Cell activation, Epithelial cell, Extracellular matrix, Peptide amphiphile, RGDS peptide, Self-assembly",
author = "Zhan Huang and Newcomb, {Christina J.} and Pablo Bringas and Stupp, {Samuel I} and Snead, {Malcolm L.}",
year = "2010",
month = "12",
doi = "10.1016/j.biomaterials.2010.08.013",
language = "English",
volume = "31",
pages = "9202--9211",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "35",

}

TY - JOUR

T1 - Biological synthesis of tooth enamel instructed by an artificial matrix

AU - Huang, Zhan

AU - Newcomb, Christina J.

AU - Bringas, Pablo

AU - Stupp, Samuel I

AU - Snead, Malcolm L.

PY - 2010/12

Y1 - 2010/12

N2 - The regenerative capability of enamel, the hardest tissue in the vertebrate body, is fundamentally limited due to cell apoptosis following maturation of the tissue. Synthetic strategies to promote enamel formation have the potential to repair damage, increase the longevity of teeth and improve the understanding of the events leading to tissue formation. Using a self-assembling bioactive matrix, we demonstrate the ability to induce ectopic formation of enamel at chosen sites adjacent to a mouse incisor cultured in vivo under the kidney capsule. The resulting material reveals the highly organized, hierarchical structure of hydroxyapatite crystallites similar to native enamel. This artificially triggered formation of organized mineral demonstrates a pathway for developing cell fabricated materials for treatment of dental caries, the most ubiquitous disease in man. Additionally, the artificial matrix provides a unique tool to probe cellular mechanisms involved in tissue formation further enabling the development of tooth organ replacements.

AB - The regenerative capability of enamel, the hardest tissue in the vertebrate body, is fundamentally limited due to cell apoptosis following maturation of the tissue. Synthetic strategies to promote enamel formation have the potential to repair damage, increase the longevity of teeth and improve the understanding of the events leading to tissue formation. Using a self-assembling bioactive matrix, we demonstrate the ability to induce ectopic formation of enamel at chosen sites adjacent to a mouse incisor cultured in vivo under the kidney capsule. The resulting material reveals the highly organized, hierarchical structure of hydroxyapatite crystallites similar to native enamel. This artificially triggered formation of organized mineral demonstrates a pathway for developing cell fabricated materials for treatment of dental caries, the most ubiquitous disease in man. Additionally, the artificial matrix provides a unique tool to probe cellular mechanisms involved in tissue formation further enabling the development of tooth organ replacements.

KW - Cell activation

KW - Epithelial cell

KW - Extracellular matrix

KW - Peptide amphiphile

KW - RGDS peptide

KW - Self-assembly

UR - http://www.scopus.com/inward/record.url?scp=77957935322&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77957935322&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2010.08.013

DO - 10.1016/j.biomaterials.2010.08.013

M3 - Article

VL - 31

SP - 9202

EP - 9211

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 35

ER -