Biphasic droplet-based sample delivery of protein crystals for serial femtosecond crystallography with an x-ray free electron laser

A. Echelmeier, G. Nelson, B. G. Abdallah, D. James, S. Roy-Chowdhury, A. Tolstikova, V. Mariani, R. A. Kirian, D. Oberthüer, K. Dörner, Petra Fromme, H. N. Chapman, U. Weierstall, J. C H Spence, A. Ros

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

Serial femtosecond crystallography (SFX) laser pulses facilitate the structure determination of smaller crystals than can generally be studied by traditional synchrotron crystallographic methods. One limiting factor of SFX is the large volume of crystal suspension needed to obtain a full data set allowing structure determination. The current method of continuous sample stream injection into an X-ray free electron laser (XFEL) requires milliliters of sample and wastes the majority of the protein crystals grown under time consuming conditions. We propose the utilization of aqueous droplets as an efficient method for protein crystal delivery into an XFEL for structure determination by SFX.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1374-1376
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: Oct 25 2015Oct 29 2015

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period10/25/1510/29/15

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Keywords

  • Droplets
  • Microfluidics
  • X-ray Crystallography

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Echelmeier, A., Nelson, G., Abdallah, B. G., James, D., Roy-Chowdhury, S., Tolstikova, A., Mariani, V., Kirian, R. A., Oberthüer, D., Dörner, K., Fromme, P., Chapman, H. N., Weierstall, U., Spence, J. C. H., & Ros, A. (2015). Biphasic droplet-based sample delivery of protein crystals for serial femtosecond crystallography with an x-ray free electron laser. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1374-1376). Chemical and Biological Microsystems Society.