Blue copper proteins. Synthesis, chemistry, and spectroscopy of CuIN3(SR) and CuIIN3(SR) active site approximations

Jeffery S. Thompson, Tobin J Marks, James A. Ibers

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Abstract

The cuprous complexes K[Cu(HB(3,5-Me2Pz)3)(SR)] (SR = p-nitrobenzenethiolatc or O-ethylcysteinate; HB(3,5-Me2pz)3 = hydrotris(3,5-dimethyl-1-pyrazolyl)borate) can be prepared by the reaction of Cu(SR) or [Cu(SR)]-(ClO4) with KHB(3,5-Me2pz)3 at room temperature. The structure of the complex with SR = p-nitrobenzenethiolate has been determined by X-ray diffraction methods. The complex crystallizes in the triclinic space group Ci1 -P1 with two molecules in a unit cell of dimensions a = 10.60 (2) Å, b = 18.17 (4) Å, c = 10.33 (4) Å, α = 93.57 (5)°, β = 116.20 (7)°, and γ = 71.89 (5)°. Least-squares refinement of the 117 variables has led to a value of the conventional R index (on F) of 0.092 for 678 independent reflections having Fo2 > 3σ(Fo2). The geometry about the Cu1 atom, which is coordinated to three nitrogen atoms and one sulfur atom, is trigonally distorted tetrahedral. The cupric complexes Cu(HB(3,5-Me2Pz)3)(SR) (SR = p-nitrobenzene-thiolate or O-ethylcysteinate) have been prepared by the reaction of KHB(3,5-Me2pz)3 with the corresponding [Cu(SR)]-(ClO4) derivatives at -78°C. UV-visible, laser Raman, and EPR data are used to show that the CuII complexes have a very similar structure to that of the Cu1 complex characterized by X-ray diffraction methods. The CuII complex Cu(HB(3,5-Me2Pz)3(OR) (OR = p-nitrobenzenephenolate, OC6H4NO2) was prepared by the reaction of NaOC6H4NO2·2H2O with CuBr(HB(3,5-Me2pz)3 in tetrahydrofuran at room temperature. The UV-visible, laser Raman, and EPR data for this complex are used in the interpretation of the spectra of the CuIN3(SR) and CuIIN3(SR) complexes. The spectral properties of the CuIIN3(SR) complexes are similar to those of the blue (type 1) copper proteins and allow certain definite conclusions to be drawn concerning the origin of the distinctive UV-visible, resonance Raman, and EPR spectral features of the proteins.

Original languageEnglish
Pages (from-to)4180-4192
Number of pages13
JournalJournal of the American Chemical Society
Volume101
Issue number15
Publication statusPublished - 1979

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X-Ray Diffraction
Paramagnetic resonance
Copper
Catalytic Domain
Spectrum Analysis
Lasers
Spectroscopy
Proteins
Atoms
Borates
Temperature
Least-Squares Analysis
Sulfur
X ray diffraction
Nitrobenzene
Nitrogen
Derivatives
Molecules
Geometry
perchlorate

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Blue copper proteins. Synthesis, chemistry, and spectroscopy of CuIN3(SR) and CuIIN3(SR) active site approximations. / Thompson, Jeffery S.; Marks, Tobin J; Ibers, James A.

In: Journal of the American Chemical Society, Vol. 101, No. 15, 1979, p. 4180-4192.

Research output: Contribution to journalArticle

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title = "Blue copper proteins. Synthesis, chemistry, and spectroscopy of CuIN3(SR) and CuIIN3(SR) active site approximations",
abstract = "The cuprous complexes K[Cu(HB(3,5-Me2Pz)3)(SR)] (SR = p-nitrobenzenethiolatc or O-ethylcysteinate; HB(3,5-Me2pz)3 = hydrotris(3,5-dimethyl-1-pyrazolyl)borate) can be prepared by the reaction of Cu(SR) or [Cu(SR)]-(ClO4) with KHB(3,5-Me2pz)3 at room temperature. The structure of the complex with SR = p-nitrobenzenethiolate has been determined by X-ray diffraction methods. The complex crystallizes in the triclinic space group Ci1 -P1 with two molecules in a unit cell of dimensions a = 10.60 (2) {\AA}, b = 18.17 (4) {\AA}, c = 10.33 (4) {\AA}, α = 93.57 (5)°, β = 116.20 (7)°, and γ = 71.89 (5)°. Least-squares refinement of the 117 variables has led to a value of the conventional R index (on F) of 0.092 for 678 independent reflections having Fo2 > 3σ(Fo2). The geometry about the Cu1 atom, which is coordinated to three nitrogen atoms and one sulfur atom, is trigonally distorted tetrahedral. The cupric complexes Cu(HB(3,5-Me2Pz)3)(SR) (SR = p-nitrobenzene-thiolate or O-ethylcysteinate) have been prepared by the reaction of KHB(3,5-Me2pz)3 with the corresponding [Cu(SR)]-(ClO4) derivatives at -78°C. UV-visible, laser Raman, and EPR data are used to show that the CuII complexes have a very similar structure to that of the Cu1 complex characterized by X-ray diffraction methods. The CuII complex Cu(HB(3,5-Me2Pz)3(OR) (OR = p-nitrobenzenephenolate, OC6H4NO2) was prepared by the reaction of NaOC6H4NO2·2H2O with CuBr(HB(3,5-Me2pz)3 in tetrahydrofuran at room temperature. The UV-visible, laser Raman, and EPR data for this complex are used in the interpretation of the spectra of the CuIN3(SR) and CuIIN3(SR) complexes. The spectral properties of the CuIIN3(SR) complexes are similar to those of the blue (type 1) copper proteins and allow certain definite conclusions to be drawn concerning the origin of the distinctive UV-visible, resonance Raman, and EPR spectral features of the proteins.",
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T1 - Blue copper proteins. Synthesis, chemistry, and spectroscopy of CuIN3(SR) and CuIIN3(SR) active site approximations

AU - Thompson, Jeffery S.

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N2 - The cuprous complexes K[Cu(HB(3,5-Me2Pz)3)(SR)] (SR = p-nitrobenzenethiolatc or O-ethylcysteinate; HB(3,5-Me2pz)3 = hydrotris(3,5-dimethyl-1-pyrazolyl)borate) can be prepared by the reaction of Cu(SR) or [Cu(SR)]-(ClO4) with KHB(3,5-Me2pz)3 at room temperature. The structure of the complex with SR = p-nitrobenzenethiolate has been determined by X-ray diffraction methods. The complex crystallizes in the triclinic space group Ci1 -P1 with two molecules in a unit cell of dimensions a = 10.60 (2) Å, b = 18.17 (4) Å, c = 10.33 (4) Å, α = 93.57 (5)°, β = 116.20 (7)°, and γ = 71.89 (5)°. Least-squares refinement of the 117 variables has led to a value of the conventional R index (on F) of 0.092 for 678 independent reflections having Fo2 > 3σ(Fo2). The geometry about the Cu1 atom, which is coordinated to three nitrogen atoms and one sulfur atom, is trigonally distorted tetrahedral. The cupric complexes Cu(HB(3,5-Me2Pz)3)(SR) (SR = p-nitrobenzene-thiolate or O-ethylcysteinate) have been prepared by the reaction of KHB(3,5-Me2pz)3 with the corresponding [Cu(SR)]-(ClO4) derivatives at -78°C. UV-visible, laser Raman, and EPR data are used to show that the CuII complexes have a very similar structure to that of the Cu1 complex characterized by X-ray diffraction methods. The CuII complex Cu(HB(3,5-Me2Pz)3(OR) (OR = p-nitrobenzenephenolate, OC6H4NO2) was prepared by the reaction of NaOC6H4NO2·2H2O with CuBr(HB(3,5-Me2pz)3 in tetrahydrofuran at room temperature. The UV-visible, laser Raman, and EPR data for this complex are used in the interpretation of the spectra of the CuIN3(SR) and CuIIN3(SR) complexes. The spectral properties of the CuIIN3(SR) complexes are similar to those of the blue (type 1) copper proteins and allow certain definite conclusions to be drawn concerning the origin of the distinctive UV-visible, resonance Raman, and EPR spectral features of the proteins.

AB - The cuprous complexes K[Cu(HB(3,5-Me2Pz)3)(SR)] (SR = p-nitrobenzenethiolatc or O-ethylcysteinate; HB(3,5-Me2pz)3 = hydrotris(3,5-dimethyl-1-pyrazolyl)borate) can be prepared by the reaction of Cu(SR) or [Cu(SR)]-(ClO4) with KHB(3,5-Me2pz)3 at room temperature. The structure of the complex with SR = p-nitrobenzenethiolate has been determined by X-ray diffraction methods. The complex crystallizes in the triclinic space group Ci1 -P1 with two molecules in a unit cell of dimensions a = 10.60 (2) Å, b = 18.17 (4) Å, c = 10.33 (4) Å, α = 93.57 (5)°, β = 116.20 (7)°, and γ = 71.89 (5)°. Least-squares refinement of the 117 variables has led to a value of the conventional R index (on F) of 0.092 for 678 independent reflections having Fo2 > 3σ(Fo2). The geometry about the Cu1 atom, which is coordinated to three nitrogen atoms and one sulfur atom, is trigonally distorted tetrahedral. The cupric complexes Cu(HB(3,5-Me2Pz)3)(SR) (SR = p-nitrobenzene-thiolate or O-ethylcysteinate) have been prepared by the reaction of KHB(3,5-Me2pz)3 with the corresponding [Cu(SR)]-(ClO4) derivatives at -78°C. UV-visible, laser Raman, and EPR data are used to show that the CuII complexes have a very similar structure to that of the Cu1 complex characterized by X-ray diffraction methods. The CuII complex Cu(HB(3,5-Me2Pz)3(OR) (OR = p-nitrobenzenephenolate, OC6H4NO2) was prepared by the reaction of NaOC6H4NO2·2H2O with CuBr(HB(3,5-Me2pz)3 in tetrahydrofuran at room temperature. The UV-visible, laser Raman, and EPR data for this complex are used in the interpretation of the spectra of the CuIN3(SR) and CuIIN3(SR) complexes. The spectral properties of the CuIIN3(SR) complexes are similar to those of the blue (type 1) copper proteins and allow certain definite conclusions to be drawn concerning the origin of the distinctive UV-visible, resonance Raman, and EPR spectral features of the proteins.

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