Boosting autofermentation rates and product yields with sodium stress cycling

Application to production of renewable fuels by cyanobacteria

Damian Carrieri, Dariya Momot, Ian A. Brasg, Gennady Ananyev, Oliver Lenz, Donald A. Bryant, G Charles Dismukes

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Sodium concentration cycling was examined as a new strategy forredistributing carbon storage products and increasing autofermentative product yields following photosynthetic carbon fixation in the cyanobacterium Arthrospira (Spirulina) maxima. The salt-tolerant hypercarbonate strain CS-328 was grown in a medium containing 0.24 to 1.24 M sodium, resulting in increased biosynthesis of soluble carbohydrates to up to 50% of the dry weight at 1.24 M sodium. Hypoionic stress during dark anaerobic metabolism (autofermentation) was induced by resuspending filaments in low-sodium (bi)carbonate buffer (0.21 M), which resulted in accelerated autofermentation rates. For cells grown in 1.24 M NaCl, the fermentative yields of acetate, ethanol, andformate increase substantially to 1.56, 0.75, and 1.54 mmol/(g [dry weight] of cells · day), respectively (36-, 121-, and 6-fold increases in rates relative to cells grown in 0.24 M NaCl). Catabolism of endogenous carbohydrate increased by approximately 2-fold uponhypoionic stress. For cultures grown at all salt concentrations, hydrogen was produced, but its yield did not correlate with increased catabolism of soluble carbohydrates. Instead, ethanol excretion becomes a preferred route for fermentative NADH reoxidation, together with intracellular accumulation of reduced products of acetyl coenzyme A (acetyl-CoA) formation when cells are hypoionically stressed. In the absence of hypoionic stress,hydrogen production is a major beneficial pathway for NAD+ regeneration without wasting carbonintermediates such as ethanol derived from acetyl- CoA. This switch presumably improves the overall cellular economy by retaining carbonwithin the cell until aerobic conditions return and the acetyl unit can be used for biosynthesis or oxidized via respiration for a much greater energy return.

Original languageEnglish
Pages (from-to)6455-6462
Number of pages8
JournalApplied and Environmental Microbiology
Volume76
Issue number19
DOIs
Publication statusPublished - Oct 2010

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Cyanobacteria
cyanobacterium
Sodium
sodium
carbohydrate
ethanol
catabolism
Spirulina
Acetyl Coenzyme A
Ethanol
NAD (coenzyme)
Carbohydrates
carbohydrates
NAD
hydrogen
cells
Hydrogen
fold
salt
Salts

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

Boosting autofermentation rates and product yields with sodium stress cycling : Application to production of renewable fuels by cyanobacteria. / Carrieri, Damian; Momot, Dariya; Brasg, Ian A.; Ananyev, Gennady; Lenz, Oliver; Bryant, Donald A.; Dismukes, G Charles.

In: Applied and Environmental Microbiology, Vol. 76, No. 19, 10.2010, p. 6455-6462.

Research output: Contribution to journalArticle

Carrieri, Damian ; Momot, Dariya ; Brasg, Ian A. ; Ananyev, Gennady ; Lenz, Oliver ; Bryant, Donald A. ; Dismukes, G Charles. / Boosting autofermentation rates and product yields with sodium stress cycling : Application to production of renewable fuels by cyanobacteria. In: Applied and Environmental Microbiology. 2010 ; Vol. 76, No. 19. pp. 6455-6462.
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