Abstract
We report on an entirely man-made nano-bio architecture fabricated through noncovalent assembly of a cell-free expressed transmembrane proton pump and TiO2 semiconductor nanoparticles as an efficient nanophotocatalyst for H2 evolution. The system produces hydrogen at a turnover of about 240 μmol of H2 (μmol protein)-1 h-1 and 17.74 mmol of H2 (μmol protein)-1 h-1 under monochromatic green and white light, respectively, at ambient conditions, in water at neutral pH and room temperature, with methanol as a sacrificial electron donor. Robustness and flexibility of this approach allow for systemic manipulation at the nanoparticle-bio interface toward directed evolution of energy transformation materials and artificial systems.
Original language | English |
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Pages (from-to) | 6739-6745 |
Number of pages | 7 |
Journal | ACS nano |
Volume | 11 |
Issue number | 7 |
DOIs | |
Publication status | Published - Jul 25 2017 |
Keywords
- cell-free expression
- hydrogen
- nanodisc TiO nanoparticles
- noncovalent assembly
- proton pump
- purple membranes
- solar-to-fuel energy conversion
ASJC Scopus subject areas
- Materials Science(all)
- Engineering(all)
- Physics and Astronomy(all)