Cell-Free Synthetic Biology Chassis for Nanocatalytic Photon-to-Hydrogen Conversion

Peng Wang, Angela Y. Chang, Valentyn Novosad, Vladimir V. Chupin, Richard D Schaller, Elena A. Rozhkova

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We report on an entirely man-made nano-bio architecture fabricated through noncovalent assembly of a cell-free expressed transmembrane proton pump and TiO2 semiconductor nanoparticles as an efficient nanophotocatalyst for H2 evolution. The system produces hydrogen at a turnover of about 240 μmol of H2 (μmol protein)-1 h-1 and 17.74 mmol of H2 (μmol protein)-1 h-1 under monochromatic green and white light, respectively, at ambient conditions, in water at neutral pH and room temperature, with methanol as a sacrificial electron donor. Robustness and flexibility of this approach allow for systemic manipulation at the nanoparticle-bio interface toward directed evolution of energy transformation materials and artificial systems.

Original languageEnglish
Pages (from-to)6739-6745
Number of pages7
JournalACS Nano
Volume11
Issue number7
DOIs
Publication statusPublished - Jul 25 2017

Fingerprint

chassis
Chassis
biology
Hydrogen
Photons
Nanoparticles
proteins
Proteins
Proton Pumps
nanoparticles
photons
hydrogen
cells
Methanol
manipulators
flexibility
Protons
methyl alcohol
assembly
Pumps

Keywords

  • cell-free expression
  • hydrogen
  • nanodisc TiO nanoparticles
  • noncovalent assembly
  • proton pump
  • purple membranes
  • solar-to-fuel energy conversion

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Wang, P., Chang, A. Y., Novosad, V., Chupin, V. V., Schaller, R. D., & Rozhkova, E. A. (2017). Cell-Free Synthetic Biology Chassis for Nanocatalytic Photon-to-Hydrogen Conversion. ACS Nano, 11(7), 6739-6745. https://doi.org/10.1021/acsnano.7b01142

Cell-Free Synthetic Biology Chassis for Nanocatalytic Photon-to-Hydrogen Conversion. / Wang, Peng; Chang, Angela Y.; Novosad, Valentyn; Chupin, Vladimir V.; Schaller, Richard D; Rozhkova, Elena A.

In: ACS Nano, Vol. 11, No. 7, 25.07.2017, p. 6739-6745.

Research output: Contribution to journalArticle

Wang, P, Chang, AY, Novosad, V, Chupin, VV, Schaller, RD & Rozhkova, EA 2017, 'Cell-Free Synthetic Biology Chassis for Nanocatalytic Photon-to-Hydrogen Conversion', ACS Nano, vol. 11, no. 7, pp. 6739-6745. https://doi.org/10.1021/acsnano.7b01142
Wang, Peng ; Chang, Angela Y. ; Novosad, Valentyn ; Chupin, Vladimir V. ; Schaller, Richard D ; Rozhkova, Elena A. / Cell-Free Synthetic Biology Chassis for Nanocatalytic Photon-to-Hydrogen Conversion. In: ACS Nano. 2017 ; Vol. 11, No. 7. pp. 6739-6745.
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