Abstract
We report on an entirely man-made nano-bio architecture fabricated through noncovalent assembly of a cell-free expressed transmembrane proton pump and TiO2 semiconductor nanoparticles as an efficient nanophotocatalyst for H2 evolution. The system produces hydrogen at a turnover of about 240 μmol of H2 (μmol protein)-1 h-1 and 17.74 mmol of H2 (μmol protein)-1 h-1 under monochromatic green and white light, respectively, at ambient conditions, in water at neutral pH and room temperature, with methanol as a sacrificial electron donor. Robustness and flexibility of this approach allow for systemic manipulation at the nanoparticle-bio interface toward directed evolution of energy transformation materials and artificial systems.
| Original language | English |
|---|---|
| Pages (from-to) | 6739-6745 |
| Number of pages | 7 |
| Journal | ACS Nano |
| Volume | 11 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 25 2017 |
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Keywords
- cell-free expression
- hydrogen
- nanodisc TiO nanoparticles
- noncovalent assembly
- proton pump
- purple membranes
- solar-to-fuel energy conversion
ASJC Scopus subject areas
- Materials Science(all)
- Engineering(all)
- Physics and Astronomy(all)
Cite this
Cell-Free Synthetic Biology Chassis for Nanocatalytic Photon-to-Hydrogen Conversion. / Wang, Peng; Chang, Angela Y.; Novosad, Valentyn; Chupin, Vladimir V.; Schaller, Richard D; Rozhkova, Elena A.
In: ACS Nano, Vol. 11, No. 7, 25.07.2017, p. 6739-6745.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cell-Free Synthetic Biology Chassis for Nanocatalytic Photon-to-Hydrogen Conversion
AU - Wang, Peng
AU - Chang, Angela Y.
AU - Novosad, Valentyn
AU - Chupin, Vladimir V.
AU - Schaller, Richard D
AU - Rozhkova, Elena A.
PY - 2017/7/25
Y1 - 2017/7/25
N2 - We report on an entirely man-made nano-bio architecture fabricated through noncovalent assembly of a cell-free expressed transmembrane proton pump and TiO2 semiconductor nanoparticles as an efficient nanophotocatalyst for H2 evolution. The system produces hydrogen at a turnover of about 240 μmol of H2 (μmol protein)-1 h-1 and 17.74 mmol of H2 (μmol protein)-1 h-1 under monochromatic green and white light, respectively, at ambient conditions, in water at neutral pH and room temperature, with methanol as a sacrificial electron donor. Robustness and flexibility of this approach allow for systemic manipulation at the nanoparticle-bio interface toward directed evolution of energy transformation materials and artificial systems.
AB - We report on an entirely man-made nano-bio architecture fabricated through noncovalent assembly of a cell-free expressed transmembrane proton pump and TiO2 semiconductor nanoparticles as an efficient nanophotocatalyst for H2 evolution. The system produces hydrogen at a turnover of about 240 μmol of H2 (μmol protein)-1 h-1 and 17.74 mmol of H2 (μmol protein)-1 h-1 under monochromatic green and white light, respectively, at ambient conditions, in water at neutral pH and room temperature, with methanol as a sacrificial electron donor. Robustness and flexibility of this approach allow for systemic manipulation at the nanoparticle-bio interface toward directed evolution of energy transformation materials and artificial systems.
KW - cell-free expression
KW - hydrogen
KW - nanodisc TiO nanoparticles
KW - noncovalent assembly
KW - proton pump
KW - purple membranes
KW - solar-to-fuel energy conversion
UR - http://www.scopus.com/inward/record.url?scp=85026312012&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85026312012&partnerID=8YFLogxK
U2 - 10.1021/acsnano.7b01142
DO - 10.1021/acsnano.7b01142
M3 - Article
VL - 11
SP - 6739
EP - 6745
JO - ACS Nano
JF - ACS Nano
SN - 1936-0851
IS - 7
ER -