Cell-Free Synthetic Biology Chassis for Nanocatalytic Photon-to-Hydrogen Conversion

Peng Wang; Angela Y. Chang; Valentyn Novosad; Vladimir V. Chupin; Richard D. Schaller; Elena A. Rozhkova

doi:10.1021/acsnano.7b01142

Cell-Free Synthetic Biology Chassis for Nanocatalytic Photon-to-Hydrogen Conversion

Peng Wang, Angela Y. Chang, Valentyn Novosad, Vladimir V. Chupin, Richard D. Schaller, Elena A. Rozhkova

Northwestern University, Argonne National Laboratory

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

We report on an entirely man-made nano-bio architecture fabricated through noncovalent assembly of a cell-free expressed transmembrane proton pump and TiO₂ semiconductor nanoparticles as an efficient nanophotocatalyst for H₂ evolution. The system produces hydrogen at a turnover of about 240 μmol of H₂ (μmol protein)^-1 h^-1 and 17.74 mmol of H₂ (μmol protein)^-1 h^-1 under monochromatic green and white light, respectively, at ambient conditions, in water at neutral pH and room temperature, with methanol as a sacrificial electron donor. Robustness and flexibility of this approach allow for systemic manipulation at the nanoparticle-bio interface toward directed evolution of energy transformation materials and artificial systems.

Original language	English
Pages (from-to)	6739-6745
Number of pages	7
Journal	ACS nano
Volume	11
Issue number	7
DOIs	https://doi.org/10.1021/acsnano.7b01142
Publication status	Published - Jul 25 2017

Keywords

cell-free expression
hydrogen
nanodisc TiO nanoparticles
noncovalent assembly
proton pump
purple membranes
solar-to-fuel energy conversion

ASJC Scopus subject areas

Materials Science(all)
Engineering(all)
Physics and Astronomy(all)

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abstract = "We report on an entirely man-made nano-bio architecture fabricated through noncovalent assembly of a cell-free expressed transmembrane proton pump and TiO2 semiconductor nanoparticles as an efficient nanophotocatalyst for H2 evolution. The system produces hydrogen at a turnover of about 240 μmol of H2 (μmol protein)-1 h-1 and 17.74 mmol of H2 (μmol protein)-1 h-1 under monochromatic green and white light, respectively, at ambient conditions, in water at neutral pH and room temperature, with methanol as a sacrificial electron donor. Robustness and flexibility of this approach allow for systemic manipulation at the nanoparticle-bio interface toward directed evolution of energy transformation materials and artificial systems.",

keywords = "cell-free expression, hydrogen, nanodisc TiO nanoparticles, noncovalent assembly, proton pump, purple membranes, solar-to-fuel energy conversion",

author = "Peng Wang and Chang, {Angela Y.} and Valentyn Novosad and Chupin, {Vladimir V.} and Schaller, {Richard D.} and Rozhkova, {Elena A.}",

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AU - Wang, Peng

AU - Chang, Angela Y.

AU - Novosad, Valentyn

AU - Chupin, Vladimir V.

AU - Schaller, Richard D.

AU - Rozhkova, Elena A.

PY - 2017/7/25

Y1 - 2017/7/25

N2 - We report on an entirely man-made nano-bio architecture fabricated through noncovalent assembly of a cell-free expressed transmembrane proton pump and TiO2 semiconductor nanoparticles as an efficient nanophotocatalyst for H2 evolution. The system produces hydrogen at a turnover of about 240 μmol of H2 (μmol protein)-1 h-1 and 17.74 mmol of H2 (μmol protein)-1 h-1 under monochromatic green and white light, respectively, at ambient conditions, in water at neutral pH and room temperature, with methanol as a sacrificial electron donor. Robustness and flexibility of this approach allow for systemic manipulation at the nanoparticle-bio interface toward directed evolution of energy transformation materials and artificial systems.

AB - We report on an entirely man-made nano-bio architecture fabricated through noncovalent assembly of a cell-free expressed transmembrane proton pump and TiO2 semiconductor nanoparticles as an efficient nanophotocatalyst for H2 evolution. The system produces hydrogen at a turnover of about 240 μmol of H2 (μmol protein)-1 h-1 and 17.74 mmol of H2 (μmol protein)-1 h-1 under monochromatic green and white light, respectively, at ambient conditions, in water at neutral pH and room temperature, with methanol as a sacrificial electron donor. Robustness and flexibility of this approach allow for systemic manipulation at the nanoparticle-bio interface toward directed evolution of energy transformation materials and artificial systems.

KW - cell-free expression

KW - hydrogen

KW - nanodisc TiO nanoparticles

KW - noncovalent assembly

KW - proton pump

KW - purple membranes

KW - solar-to-fuel energy conversion

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Cell-Free Synthetic Biology Chassis for Nanocatalytic Photon-to-Hydrogen Conversion

Abstract

Keywords

ASJC Scopus subject areas

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