Cell nucleus penetration by quantum dots induced by nuclear staining organic fluorophore and UV-irradiation

Yang Xu, Qiangbin Wang, Ping He, Qiaomei Dong, Fang Liu, Yan Liu, Lin Lin, Hao Yan, Xiaohang Zhao

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A study was conducted to report the observations and systematic investigations of mercaptopropionic-acid-functionalized quantum dots (MPA-QD) into nuclei in the presence of a nuclear staining agent,4',6-diamidino-2- phenylindole (DAPI) and ultraviolet (UV) excitation. The study involved the culture of human cancer cells in the presence of green or red MPA-QDs, or incubated with antibody conjugated green QDs. The cells were also stained by DAPI,an organic dye specific for staining nuclei before imaging by fluorescence microscopy. It was observed that both green and red MPA-QDs are taken up into the the cytoplasm of live cells. It was also observed that the green MPA-QDs can move rapidly from the cytoplasm into nuclei in the presence of DAPI after UV excitation.

Original languageEnglish
Pages (from-to)3468-3473
Number of pages6
JournalAdvanced Materials
Volume20
Issue number18
DOIs
Publication statusPublished - Sep 17 2008

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

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