Cellular uptake and cytotoxicity of a near-IR fluorescent corrole-TiO2 nanoconjugate

Carl M. Blumenfeld, Bryce F. Sadtler, G. Esteban Fernandez, Lily Dara, Cathie Nguyen, Felix Alonso-Valenteen, Lali Medina-Kauwe, Rex A. Moats, Nathan S Lewis, Robert H. Grubbs, Harry B. Gray, Karn Sorasaenee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We are investigating the biological and biomedical imaging roles and impacts of fluorescent metallocorrole-TiO2 nanoconjugates as potential near-infrared optical contrast agents in vitro in cancer and normal cell lines. The TiO2 nanoconjugate labeled with the small molecule 2,17-bis(chlorosulfonyl)-5,10,15-tris(pentafluorophenyl)corrolato aluminum(III) (1-Al-TiO2) was prepared. The nanoparticle 1-Al-TiO2 was characterized by transmission electron microscopy (TEM) and integrating-sphere electronic absorption spectroscopy. TEM images of three different samples of TiO2 nanoparticles (bare, H2O2 etched, and 1-Al functionalized) showed similarity in shapes and sizes with an average diameter of 29 nm for 1-Al-TiO2. Loading of 1-Al on the TiO2 surfaces was determined to be ca. 20-40 mg 1-Al/g TiO2. Confocal fluorescence microscopy (CFM) studies of luciferase-transfected primary human glioblastoma U87-Luc cells treated with the nanoconjugate 1-Al-TiO2 as the contrast agent in various concentrations were performed. The CFM images revealed that 1-Al-TiO2 was found inside the cancer cells even at low doses (0.02-2 μg/mL) and localized in the cytosol. Bioluminescence studies of the U87-Luc cells exposed to various amounts of 1-Al-TiO2 showed minimal cytotoxic effects even at higher doses (2-2000 μg/mL) after 24 h. A similar observation was made using primary mouse hepatocytes (PMH) treated with 1-Al-TiO2 at low doses (0.0003-3 μg/mL). Longer incubation times (after 48 and 72 h for U87-Luc) and higher doses (> 20 μg/mL 1-Al-TiO2 for U87-Luc and > 3 μg/mL 1-Al-TiO2 for PMH) showed decreased cell viability.

Original languageEnglish
Pages (from-to)39-44
Number of pages6
JournalJournal of Inorganic Biochemistry
Volume140
DOIs
Publication statusPublished - 2014

Fingerprint

Nanoconjugates
Cytotoxicity
Confocal microscopy
Fluorescence microscopy
Cells
Transmission Electron Microscopy
Fluorescence Microscopy
Confocal Microscopy
Nanoparticles
Contrast Media
Hepatocytes
Bioluminescence
Transmission electron microscopy
Glioblastoma
Luciferases
Aluminum
Absorption spectroscopy
Cytosol
Neoplasms
Spectrum Analysis

Keywords

  • Cellular uptake
  • Corrole
  • Cytotoxic effect
  • Fluorescence
  • Nanoconjugate

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry
  • Medicine(all)

Cite this

Blumenfeld, C. M., Sadtler, B. F., Fernandez, G. E., Dara, L., Nguyen, C., Alonso-Valenteen, F., ... Sorasaenee, K. (2014). Cellular uptake and cytotoxicity of a near-IR fluorescent corrole-TiO2 nanoconjugate. Journal of Inorganic Biochemistry, 140, 39-44. https://doi.org/10.1016/j.jinorgbio.2014.06.015

Cellular uptake and cytotoxicity of a near-IR fluorescent corrole-TiO2 nanoconjugate. / Blumenfeld, Carl M.; Sadtler, Bryce F.; Fernandez, G. Esteban; Dara, Lily; Nguyen, Cathie; Alonso-Valenteen, Felix; Medina-Kauwe, Lali; Moats, Rex A.; Lewis, Nathan S; Grubbs, Robert H.; Gray, Harry B.; Sorasaenee, Karn.

In: Journal of Inorganic Biochemistry, Vol. 140, 2014, p. 39-44.

Research output: Contribution to journalArticle

Blumenfeld, CM, Sadtler, BF, Fernandez, GE, Dara, L, Nguyen, C, Alonso-Valenteen, F, Medina-Kauwe, L, Moats, RA, Lewis, NS, Grubbs, RH, Gray, HB & Sorasaenee, K 2014, 'Cellular uptake and cytotoxicity of a near-IR fluorescent corrole-TiO2 nanoconjugate', Journal of Inorganic Biochemistry, vol. 140, pp. 39-44. https://doi.org/10.1016/j.jinorgbio.2014.06.015
Blumenfeld, Carl M. ; Sadtler, Bryce F. ; Fernandez, G. Esteban ; Dara, Lily ; Nguyen, Cathie ; Alonso-Valenteen, Felix ; Medina-Kauwe, Lali ; Moats, Rex A. ; Lewis, Nathan S ; Grubbs, Robert H. ; Gray, Harry B. ; Sorasaenee, Karn. / Cellular uptake and cytotoxicity of a near-IR fluorescent corrole-TiO2 nanoconjugate. In: Journal of Inorganic Biochemistry. 2014 ; Vol. 140. pp. 39-44.
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