Characterization of thiosulfate reductase from Pyrobaculum aerophilum heterologously produced in Pyrococcus furiosus

Dominik K. Haja, Chang Hao Wu, Farris L. Poole, John Sugar, Samuel G. Williams, Anne Katherine Jones, Michael W.W. Adams

Research output: Contribution to journalArticle

Abstract

The genome of the archaeon Pyrobaculum aerophilum (Topt ~ 100 °C) contains an operon (PAE2859–2861) encoding a putative pyranopterin-containing oxidoreductase of unknown function and metal content. These genes (with one gene modified to encode a His-affinity tag) were inserted into the fermentative anaerobic archaeon, Pyrococcus furiosus (Topt ~ 100 °C). Dye-linked assays of cytoplasmic extracts from recombinant P. furiosus show that the P. aerophilum enzyme is a thiosulfate reductase (Tsr) and reduces thiosulfate but not polysulfide. The enzyme (Tsr–Mo) from molybdenum-grown cells contains Mo (Mo:W = 9:1) while the enzyme (Tsr–W) from tungsten-grown cells contains mainly W (Mo:W = 1:6). Purified Tsr–Mo has over ten times the activity (Vmax = 1580 vs. 141 µmol min−1 mg−1) and twice the affinity for thiosulfate (Km = ~ 100 vs. ~ 200 μM) than Tsr–W and is reduced at a lower potential (Epeak = − 255 vs − 402 mV). Tsr–Mo and Tsr–W proteins are heterodimers lacking the membrane anchor subunit (PAE2861). Recombinant P. furiosus expressing P. aerophilum Tsr could not use thiosulfate as a terminal electron acceptor. P. furiosus contains five pyranopterin-containing enzymes, all of which utilize W. P. aerophilum Tsr–Mo is the first example of an active Mo-containing enzyme produced in P. furiosus.

Original languageEnglish
JournalExtremophiles
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Pyrobaculum
Pyrococcus furiosus
Thiosulfates
Enzymes
Archaea
Tungsten
Molybdenum
Operon
Genes
Oxidoreductases
Coloring Agents
Metals
thiosulfate-dithiol sulfurtransferase
Genome
Electrons
Membranes

Keywords

  • Archaea
  • Hyperthermophiles
  • Metabolism
  • Metalloenzyme
  • Pyranopterin
  • Recombinant proteins

ASJC Scopus subject areas

  • Microbiology
  • Molecular Medicine

Cite this

Characterization of thiosulfate reductase from Pyrobaculum aerophilum heterologously produced in Pyrococcus furiosus. / Haja, Dominik K.; Wu, Chang Hao; Poole, Farris L.; Sugar, John; Williams, Samuel G.; Jones, Anne Katherine; Adams, Michael W.W.

In: Extremophiles, 01.01.2019.

Research output: Contribution to journalArticle

Haja, Dominik K. ; Wu, Chang Hao ; Poole, Farris L. ; Sugar, John ; Williams, Samuel G. ; Jones, Anne Katherine ; Adams, Michael W.W. / Characterization of thiosulfate reductase from Pyrobaculum aerophilum heterologously produced in Pyrococcus furiosus. In: Extremophiles. 2019.
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abstract = "The genome of the archaeon Pyrobaculum aerophilum (Topt ~ 100 °C) contains an operon (PAE2859–2861) encoding a putative pyranopterin-containing oxidoreductase of unknown function and metal content. These genes (with one gene modified to encode a His-affinity tag) were inserted into the fermentative anaerobic archaeon, Pyrococcus furiosus (Topt ~ 100 °C). Dye-linked assays of cytoplasmic extracts from recombinant P. furiosus show that the P. aerophilum enzyme is a thiosulfate reductase (Tsr) and reduces thiosulfate but not polysulfide. The enzyme (Tsr–Mo) from molybdenum-grown cells contains Mo (Mo:W = 9:1) while the enzyme (Tsr–W) from tungsten-grown cells contains mainly W (Mo:W = 1:6). Purified Tsr–Mo has over ten times the activity (Vmax = 1580 vs. 141 µmol min−1 mg−1) and twice the affinity for thiosulfate (Km = ~ 100 vs. ~ 200 μM) than Tsr–W and is reduced at a lower potential (Epeak = − 255 vs − 402 mV). Tsr–Mo and Tsr–W proteins are heterodimers lacking the membrane anchor subunit (PAE2861). Recombinant P. furiosus expressing P. aerophilum Tsr could not use thiosulfate as a terminal electron acceptor. P. furiosus contains five pyranopterin-containing enzymes, all of which utilize W. P. aerophilum Tsr–Mo is the first example of an active Mo-containing enzyme produced in P. furiosus.",
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AB - The genome of the archaeon Pyrobaculum aerophilum (Topt ~ 100 °C) contains an operon (PAE2859–2861) encoding a putative pyranopterin-containing oxidoreductase of unknown function and metal content. These genes (with one gene modified to encode a His-affinity tag) were inserted into the fermentative anaerobic archaeon, Pyrococcus furiosus (Topt ~ 100 °C). Dye-linked assays of cytoplasmic extracts from recombinant P. furiosus show that the P. aerophilum enzyme is a thiosulfate reductase (Tsr) and reduces thiosulfate but not polysulfide. The enzyme (Tsr–Mo) from molybdenum-grown cells contains Mo (Mo:W = 9:1) while the enzyme (Tsr–W) from tungsten-grown cells contains mainly W (Mo:W = 1:6). Purified Tsr–Mo has over ten times the activity (Vmax = 1580 vs. 141 µmol min−1 mg−1) and twice the affinity for thiosulfate (Km = ~ 100 vs. ~ 200 μM) than Tsr–W and is reduced at a lower potential (Epeak = − 255 vs − 402 mV). Tsr–Mo and Tsr–W proteins are heterodimers lacking the membrane anchor subunit (PAE2861). Recombinant P. furiosus expressing P. aerophilum Tsr could not use thiosulfate as a terminal electron acceptor. P. furiosus contains five pyranopterin-containing enzymes, all of which utilize W. P. aerophilum Tsr–Mo is the first example of an active Mo-containing enzyme produced in P. furiosus.

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