Chemically selective imaging of subcellular structure in human hepatocytes with coherent anti-stokes raman scattering (CARS) near-field scanning optical microscopy (NSOM)

Richard D. Schaller, Joseph Ziegelbauer, Lynn F. Lee, Louis H. Haber, Richard J. Saykally

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46 Citations (Scopus)

Abstract

Chemically selective imaging of human hepatocytes via intrinsic vibrational resonances with spatial resolution below the diffraction limit was carried out. The combination of coherent anti-Stoke Raman scattering (CARS) with near-field scanning optical microscopy (NSOM) was used for the imaging. Femtosecond, near-IR pulses produced CARS signals from hepatocytes and the signal was tuned to be resonant with C-H stretching frequencies. Results showed an image contrast with an optical spatial resolution ∼128 nm.

Original languageEnglish
Pages (from-to)8489-8492
Number of pages4
JournalJournal of Physical Chemistry B
Volume106
Issue number34
DOIs
Publication statusPublished - Aug 29 2002

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

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