Chemically selective imaging of subcellular structure in human hepatocytes with coherent anti-stokes raman scattering (CARS) near-field scanning optical microscopy (NSOM)

Richard D. Schaller, Joseph Ziegelbauer, Lynn F. Lee, Louis H. Haber, Richard J. Saykally

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Chemically selective imaging of human hepatocytes via intrinsic vibrational resonances with spatial resolution below the diffraction limit was carried out. The combination of coherent anti-Stoke Raman scattering (CARS) with near-field scanning optical microscopy (NSOM) was used for the imaging. Femtosecond, near-IR pulses produced CARS signals from hepatocytes and the signal was tuned to be resonant with C-H stretching frequencies. Results showed an image contrast with an optical spatial resolution ∼128 nm.

Original languageEnglish
Pages (from-to)8489-8492
Number of pages4
JournalJournal of Physical Chemistry B
Volume106
Issue number34
DOIs
Publication statusPublished - Aug 29 2002

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

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