Abstract
Chemically selective imaging of human hepatocytes via intrinsic vibrational resonances with spatial resolution below the diffraction limit was carried out. The combination of coherent anti-Stoke Raman scattering (CARS) with near-field scanning optical microscopy (NSOM) was used for the imaging. Femtosecond, near-IR pulses produced CARS signals from hepatocytes and the signal was tuned to be resonant with C-H stretching frequencies. Results showed an image contrast with an optical spatial resolution ∼128 nm.
| Original language | English |
|---|---|
| Pages (from-to) | 8489-8492 |
| Number of pages | 4 |
| Journal | Journal of Physical Chemistry B |
| Volume | 106 |
| Issue number | 34 |
| DOIs | |
| Publication status | Published - Aug 29 2002 |
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Surfaces, Coatings and Films
- Materials Chemistry
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Schaller, R. D., Ziegelbauer, J., Lee, L. F., Haber, L. H., & Saykally, R. J. (2002). Chemically selective imaging of subcellular structure in human hepatocytes with coherent anti-stokes raman scattering (CARS) near-field scanning optical microscopy (NSOM). Journal of Physical Chemistry B, 106(34), 8489-8492. https://doi.org/10.1021/jp020855t