TY - JOUR
T1 - Chemically selective imaging of subcellular structure in human hepatocytes with coherent anti-stokes raman scattering (CARS) near-field scanning optical microscopy (NSOM)
AU - Schaller, Richard D.
AU - Ziegelbauer, Joseph
AU - Lee, Lynn F.
AU - Haber, Louis H.
AU - Saykally, Richard J.
PY - 2002/8/29
Y1 - 2002/8/29
N2 - Chemically selective imaging of human hepatocytes via intrinsic vibrational resonances with spatial resolution below the diffraction limit was carried out. The combination of coherent anti-Stoke Raman scattering (CARS) with near-field scanning optical microscopy (NSOM) was used for the imaging. Femtosecond, near-IR pulses produced CARS signals from hepatocytes and the signal was tuned to be resonant with C-H stretching frequencies. Results showed an image contrast with an optical spatial resolution ∼128 nm.
AB - Chemically selective imaging of human hepatocytes via intrinsic vibrational resonances with spatial resolution below the diffraction limit was carried out. The combination of coherent anti-Stoke Raman scattering (CARS) with near-field scanning optical microscopy (NSOM) was used for the imaging. Femtosecond, near-IR pulses produced CARS signals from hepatocytes and the signal was tuned to be resonant with C-H stretching frequencies. Results showed an image contrast with an optical spatial resolution ∼128 nm.
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U2 - 10.1021/jp020855t
DO - 10.1021/jp020855t
M3 - Article
AN - SCOPUS:0037194977
VL - 106
SP - 8489
EP - 8492
JO - Journal of Physical Chemistry B
JF - Journal of Physical Chemistry B
SN - 1520-6106
IS - 34
ER -