Abstract
Chemically selective imaging of human hepatocytes via intrinsic vibrational resonances with spatial resolution below the diffraction limit was carried out. The combination of coherent anti-Stoke Raman scattering (CARS) with near-field scanning optical microscopy (NSOM) was used for the imaging. Femtosecond, near-IR pulses produced CARS signals from hepatocytes and the signal was tuned to be resonant with C-H stretching frequencies. Results showed an image contrast with an optical spatial resolution ∼128 nm.
| Original language | English |
|---|---|
| Pages (from-to) | 8489-8492 |
| Number of pages | 4 |
| Journal | Journal of Physical Chemistry B |
| Volume | 106 |
| Issue number | 34 |
| DOIs | |
| Publication status | Published - Aug 29 2002 |
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ASJC Scopus subject areas
- Physical and Theoretical Chemistry
Cite this
Chemically selective imaging of subcellular structure in human hepatocytes with coherent anti-stokes raman scattering (CARS) near-field scanning optical microscopy (NSOM). / Schaller, Richard D; Ziegelbauer, Joseph; Lee, Lynn F.; Haber, Louis H.; Saykally, Richard J.
In: Journal of Physical Chemistry B, Vol. 106, No. 34, 29.08.2002, p. 8489-8492.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chemically selective imaging of subcellular structure in human hepatocytes with coherent anti-stokes raman scattering (CARS) near-field scanning optical microscopy (NSOM)
AU - Schaller, Richard D
AU - Ziegelbauer, Joseph
AU - Lee, Lynn F.
AU - Haber, Louis H.
AU - Saykally, Richard J.
PY - 2002/8/29
Y1 - 2002/8/29
N2 - Chemically selective imaging of human hepatocytes via intrinsic vibrational resonances with spatial resolution below the diffraction limit was carried out. The combination of coherent anti-Stoke Raman scattering (CARS) with near-field scanning optical microscopy (NSOM) was used for the imaging. Femtosecond, near-IR pulses produced CARS signals from hepatocytes and the signal was tuned to be resonant with C-H stretching frequencies. Results showed an image contrast with an optical spatial resolution ∼128 nm.
AB - Chemically selective imaging of human hepatocytes via intrinsic vibrational resonances with spatial resolution below the diffraction limit was carried out. The combination of coherent anti-Stoke Raman scattering (CARS) with near-field scanning optical microscopy (NSOM) was used for the imaging. Femtosecond, near-IR pulses produced CARS signals from hepatocytes and the signal was tuned to be resonant with C-H stretching frequencies. Results showed an image contrast with an optical spatial resolution ∼128 nm.
UR - http://www.scopus.com/inward/record.url?scp=0037194977&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037194977&partnerID=8YFLogxK
U2 - 10.1021/jp020855t
DO - 10.1021/jp020855t
M3 - Article
VL - 106
SP - 8489
EP - 8492
JO - Journal of Physical Chemistry B Materials
JF - Journal of Physical Chemistry B Materials
SN - 1520-6106
IS - 34
ER -