Chloride binding to photosystem II in the dark is in slow exchange

Yair Shachar-Hill; Warren F. Beck; Gary W Brudvig

doi:10.1016/0014-5793(89)81035-X

Chloride binding to photosystem II in the dark is in slow exchange

Yair Shachar-Hill, Warren F. Beck, Gary W Brudvig

Yale University

Research output: Contribution to journal › Article

5 Citations (Scopus)

Abstract

We have studied the ³⁵Cl^- NMR line broadening in the presence of photosystem II (PS II) membranes from spinach in the dark. In the presence of NH₃ (which other work has shown to competitively inhibit chloride binding to PS II) we observed no decrease in ³⁵Cl^- linewidths. We conclude that binding of Cl^- to the O₂ evolving center in PS II in the dark (previously demonstrated by EPR) is in slow exchange on the NMR timescale. We assign the observed line broadening to interaction with non-specific binding sites and with free paramagnetics.

Original language	English
Pages (from-to)	184-188
Number of pages	5
Journal	FEBS Letters
Volume	254
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(89)81035-X
Publication status	Published - Aug 28 1989

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Keywords

Chloride
NMR, Cl
Photosystem II

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

Cite this

Shachar-Hill, Y., Beck, W. F., & Brudvig, G. W. (1989). Chloride binding to photosystem II in the dark is in slow exchange. FEBS Letters, 254(1-2), 184-188. https://doi.org/10.1016/0014-5793(89)81035-X

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abstract = "We have studied the 35Cl- NMR line broadening in the presence of photosystem II (PS II) membranes from spinach in the dark. In the presence of NH3 (which other work has shown to competitively inhibit chloride binding to PS II) we observed no decrease in 35Cl- linewidths. We conclude that binding of Cl- to the O2 evolving center in PS II in the dark (previously demonstrated by EPR) is in slow exchange on the NMR timescale. We assign the observed line broadening to interaction with non-specific binding sites and with free paramagnetics.",

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Access to Document

10.1016/0014-5793(89)81035-X