Abstract
We have studied the 35Cl- NMR line broadening in the presence of photosystem II (PS II) membranes from spinach in the dark. In the presence of NH3 (which other work has shown to competitively inhibit chloride binding to PS II) we observed no decrease in 35Cl- linewidths. We conclude that binding of Cl- to the O2 evolving center in PS II in the dark (previously demonstrated by EPR) is in slow exchange on the NMR timescale. We assign the observed line broadening to interaction with non-specific binding sites and with free paramagnetics.
| Original language | English |
|---|---|
| Pages (from-to) | 184-188 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 254 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Aug 28 1989 |
Keywords
- Chloride
- NMR, Cl
- Photosystem II
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Shachar-Hill, Y., Beck, W. F., & Brudvig, G. W. (1989). Chloride binding to photosystem II in the dark is in slow exchange. FEBS Letters, 254(1-2), 184-188. https://doi.org/10.1016/0014-5793(89)81035-X