Abstract
We have studied the 35Cl- NMR line broadening in the presence of photosystem II (PS II) membranes from spinach in the dark. In the presence of NH3 (which other work has shown to competitively inhibit chloride binding to PS II) we observed no decrease in 35Cl- linewidths. We conclude that binding of Cl- to the O2 evolving center in PS II in the dark (previously demonstrated by EPR) is in slow exchange on the NMR timescale. We assign the observed line broadening to interaction with non-specific binding sites and with free paramagnetics.
| Original language | English |
|---|---|
| Pages (from-to) | 184-188 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 254 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Aug 28 1989 |
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Keywords
- Chloride
- NMR, Cl
- Photosystem II
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Chloride binding to photosystem II in the dark is in slow exchange. / Shachar-Hill, Yair; Beck, Warren F.; Brudvig, Gary W.
In: FEBS Letters, Vol. 254, No. 1-2, 28.08.1989, p. 184-188.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chloride binding to photosystem II in the dark is in slow exchange
AU - Shachar-Hill, Yair
AU - Beck, Warren F.
AU - Brudvig, Gary W
PY - 1989/8/28
Y1 - 1989/8/28
N2 - We have studied the 35Cl- NMR line broadening in the presence of photosystem II (PS II) membranes from spinach in the dark. In the presence of NH3 (which other work has shown to competitively inhibit chloride binding to PS II) we observed no decrease in 35Cl- linewidths. We conclude that binding of Cl- to the O2 evolving center in PS II in the dark (previously demonstrated by EPR) is in slow exchange on the NMR timescale. We assign the observed line broadening to interaction with non-specific binding sites and with free paramagnetics.
AB - We have studied the 35Cl- NMR line broadening in the presence of photosystem II (PS II) membranes from spinach in the dark. In the presence of NH3 (which other work has shown to competitively inhibit chloride binding to PS II) we observed no decrease in 35Cl- linewidths. We conclude that binding of Cl- to the O2 evolving center in PS II in the dark (previously demonstrated by EPR) is in slow exchange on the NMR timescale. We assign the observed line broadening to interaction with non-specific binding sites and with free paramagnetics.
KW - Chloride
KW - NMR, Cl
KW - Photosystem II
UR - http://www.scopus.com/inward/record.url?scp=0024980254&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024980254&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(89)81035-X
DO - 10.1016/0014-5793(89)81035-X
M3 - Article
VL - 254
SP - 184
EP - 188
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-2
ER -