Chlorophyll a with a farnesyl tail in thermophilic cyanobacteria

Jessica M. Wiwczar, Amy M. LaFountain, Jimin Wang, Harry A. Frank, Gary W. Brudvig

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Photosystem II (PSII) of oxygenic photosynthetic organisms normally contains exclusively chlorophyll a (Chl a) as its major light-harvesting pigment. Chl a canonically consists of the chlorin headgroup with a 20-carbon, 4-isoprene unit, phytyl tail. We have examined the 1.9 Å crystal structure of PSII from thermophilic cyanobacteria reported by Shen and coworkers in 2012 (PDB accession of 3ARC/3WU2). A newly refined electron density map from this structure, presented here, reveals that some assignments of the cofactors may be different from those modeled in the 3ARC/3WU2 structure, including a specific Chl a that appears to have a truncated tail by one isoprene unit. We provide experimental evidence using high-performance liquid chromatography and mass spectrometry for a small population of Chl a esterified to a 15-carbon farnesyl tail in PSII of thermophilic cyanobacteria.

Original languageEnglish
Pages (from-to)175-182
Number of pages8
JournalPhotosynthesis Research
Volume134
Issue number2
DOIs
Publication statusPublished - Nov 1 2017

Keywords

  • CP43
  • Chlorophyll a
  • Photosystem II
  • Thermosynechococcus elongatus
  • X-ray crystal structure

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'Chlorophyll a with a farnesyl tail in thermophilic cyanobacteria'. Together they form a unique fingerprint.

  • Cite this