Chlorophyll a with a farnesyl tail in thermophilic cyanobacteria

Jessica M. Wiwczar; Amy M. LaFountain; Jimin Wang; Harry A. Frank; Gary W. Brudvig

doi:10.1007/s11120-017-0425-4

Chlorophyll a with a farnesyl tail in thermophilic cyanobacteria

Jessica M. Wiwczar, Amy M. LaFountain, Jimin Wang, Harry A. Frank, Gary W. Brudvig

Yale University

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Photosystem II (PSII) of oxygenic photosynthetic organisms normally contains exclusively chlorophyll a (Chl a) as its major light-harvesting pigment. Chl a canonically consists of the chlorin headgroup with a 20-carbon, 4-isoprene unit, phytyl tail. We have examined the 1.9 Å crystal structure of PSII from thermophilic cyanobacteria reported by Shen and coworkers in 2012 (PDB accession of 3ARC/3WU2). A newly refined electron density map from this structure, presented here, reveals that some assignments of the cofactors may be different from those modeled in the 3ARC/3WU2 structure, including a specific Chl a that appears to have a truncated tail by one isoprene unit. We provide experimental evidence using high-performance liquid chromatography and mass spectrometry for a small population of Chl a esterified to a 15-carbon farnesyl tail in PSII of thermophilic cyanobacteria.

Original language	English
Pages (from-to)	175-182
Number of pages	8
Journal	Photosynthesis Research
Volume	134
Issue number	2
DOIs	https://doi.org/10.1007/s11120-017-0425-4
Publication status	Published - Nov 1 2017

Keywords

CP43
Chlorophyll a
Photosystem II
Thermosynechococcus elongatus
X-ray crystal structure

ASJC Scopus subject areas

Biochemistry
Plant Science
Cell Biology

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@article{6e459a8ae10d4a0fac5e8ef93891bbe2,

title = "Chlorophyll a with a farnesyl tail in thermophilic cyanobacteria",

abstract = "Photosystem II (PSII) of oxygenic photosynthetic organisms normally contains exclusively chlorophyll a (Chl a) as its major light-harvesting pigment. Chl a canonically consists of the chlorin headgroup with a 20-carbon, 4-isoprene unit, phytyl tail. We have examined the 1.9 {\AA} crystal structure of PSII from thermophilic cyanobacteria reported by Shen and coworkers in 2012 (PDB accession of 3ARC/3WU2). A newly refined electron density map from this structure, presented here, reveals that some assignments of the cofactors may be different from those modeled in the 3ARC/3WU2 structure, including a specific Chl a that appears to have a truncated tail by one isoprene unit. We provide experimental evidence using high-performance liquid chromatography and mass spectrometry for a small population of Chl a esterified to a 15-carbon farnesyl tail in PSII of thermophilic cyanobacteria.",

keywords = "CP43, Chlorophyll a, Photosystem II, Thermosynechococcus elongatus, X-ray crystal structure",

author = "Wiwczar, {Jessica M.} and LaFountain, {Amy M.} and Jimin Wang and Frank, {Harry A.} and Brudvig, {Gary W.}",

note = "Funding Information: Acknowledgements This work was funded by the Department of Energy Grant DE-FG02-05ER15646 (G.W.B.). Work in the laboratory of H.A.F. was supported by grants from the National Science Foundation (MCB-1243565) and the University of Connecticut Research Foundation. Crystallographic analysis of this study was in part funded by National Institutes of Health Program Grant P01 GM022779. Funding Information: This work was funded by the Department of Energy Grant DE-FG02-05ER15646 (G.W.B.). Work in the laboratory of H.A.F. was supported by grants from the National Science Foundation (MCB-1243565) and the University of Connecticut Research Foundation. Crystallographic analysis of this study was in part funded by National Institutes of Health Program Grant P01 GM022779.",

year = "2017",

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doi = "10.1007/s11120-017-0425-4",

language = "English",

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T1 - Chlorophyll a with a farnesyl tail in thermophilic cyanobacteria

AU - Wiwczar, Jessica M.

AU - LaFountain, Amy M.

AU - Wang, Jimin

AU - Frank, Harry A.

AU - Brudvig, Gary W.

N1 - Funding Information: Acknowledgements This work was funded by the Department of Energy Grant DE-FG02-05ER15646 (G.W.B.). Work in the laboratory of H.A.F. was supported by grants from the National Science Foundation (MCB-1243565) and the University of Connecticut Research Foundation. Crystallographic analysis of this study was in part funded by National Institutes of Health Program Grant P01 GM022779. Funding Information: This work was funded by the Department of Energy Grant DE-FG02-05ER15646 (G.W.B.). Work in the laboratory of H.A.F. was supported by grants from the National Science Foundation (MCB-1243565) and the University of Connecticut Research Foundation. Crystallographic analysis of this study was in part funded by National Institutes of Health Program Grant P01 GM022779.

PY - 2017/11/1

Y1 - 2017/11/1

N2 - Photosystem II (PSII) of oxygenic photosynthetic organisms normally contains exclusively chlorophyll a (Chl a) as its major light-harvesting pigment. Chl a canonically consists of the chlorin headgroup with a 20-carbon, 4-isoprene unit, phytyl tail. We have examined the 1.9 Å crystal structure of PSII from thermophilic cyanobacteria reported by Shen and coworkers in 2012 (PDB accession of 3ARC/3WU2). A newly refined electron density map from this structure, presented here, reveals that some assignments of the cofactors may be different from those modeled in the 3ARC/3WU2 structure, including a specific Chl a that appears to have a truncated tail by one isoprene unit. We provide experimental evidence using high-performance liquid chromatography and mass spectrometry for a small population of Chl a esterified to a 15-carbon farnesyl tail in PSII of thermophilic cyanobacteria.

AB - Photosystem II (PSII) of oxygenic photosynthetic organisms normally contains exclusively chlorophyll a (Chl a) as its major light-harvesting pigment. Chl a canonically consists of the chlorin headgroup with a 20-carbon, 4-isoprene unit, phytyl tail. We have examined the 1.9 Å crystal structure of PSII from thermophilic cyanobacteria reported by Shen and coworkers in 2012 (PDB accession of 3ARC/3WU2). A newly refined electron density map from this structure, presented here, reveals that some assignments of the cofactors may be different from those modeled in the 3ARC/3WU2 structure, including a specific Chl a that appears to have a truncated tail by one isoprene unit. We provide experimental evidence using high-performance liquid chromatography and mass spectrometry for a small population of Chl a esterified to a 15-carbon farnesyl tail in PSII of thermophilic cyanobacteria.

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KW - Photosystem II

KW - Thermosynechococcus elongatus

KW - X-ray crystal structure

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