Chlorophyll a with a farnesyl tail in thermophilic cyanobacteria

Jessica M. Wiwczar, Amy M. LaFountain, Jimin Wang, Harry A. Frank, Gary W Brudvig

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Photosystem II (PSII) of oxygenic photosynthetic organisms normally contains exclusively chlorophyll a (Chl a) as its major light-harvesting pigment. Chl a canonically consists of the chlorin headgroup with a 20-carbon, 4-isoprene unit, phytyl tail. We have examined the 1.9 Å crystal structure of PSII from thermophilic cyanobacteria reported by Shen and coworkers in 2012 (PDB accession of 3ARC/3WU2). A newly refined electron density map from this structure, presented here, reveals that some assignments of the cofactors may be different from those modeled in the 3ARC/3WU2 structure, including a specific Chl a that appears to have a truncated tail by one isoprene unit. We provide experimental evidence using high-performance liquid chromatography and mass spectrometry for a small population of Chl a esterified to a 15-carbon farnesyl tail in PSII of thermophilic cyanobacteria.

Original languageEnglish
Pages (from-to)175-182
Number of pages8
JournalPhotosynthesis Research
Volume134
Issue number2
DOIs
Publication statusPublished - Nov 1 2017

Fingerprint

Cyanobacteria
Photosystem II Protein Complex
tail
photosystem II
chlorophyll
Carbon
chlorins
carbon
High performance liquid chromatography
autotrophs
crystal structure
Pigments
Carrier concentration
Mass spectrometry
Mass Spectrometry
high performance liquid chromatography
Crystal structure
pigments
High Pressure Liquid Chromatography
electrons

Keywords

  • Chlorophyll a
  • CP43
  • Photosystem II
  • Thermosynechococcus elongatus
  • X-ray crystal structure

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

Cite this

Chlorophyll a with a farnesyl tail in thermophilic cyanobacteria. / Wiwczar, Jessica M.; LaFountain, Amy M.; Wang, Jimin; Frank, Harry A.; Brudvig, Gary W.

In: Photosynthesis Research, Vol. 134, No. 2, 01.11.2017, p. 175-182.

Research output: Contribution to journalArticle

Wiwczar, Jessica M. ; LaFountain, Amy M. ; Wang, Jimin ; Frank, Harry A. ; Brudvig, Gary W. / Chlorophyll a with a farnesyl tail in thermophilic cyanobacteria. In: Photosynthesis Research. 2017 ; Vol. 134, No. 2. pp. 175-182.
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