Abstract
Photosystem II (PSII) of oxygenic photosynthetic organisms normally contains exclusively chlorophyll a (Chl a) as its major light-harvesting pigment. Chl a canonically consists of the chlorin headgroup with a 20-carbon, 4-isoprene unit, phytyl tail. We have examined the 1.9 Å crystal structure of PSII from thermophilic cyanobacteria reported by Shen and coworkers in 2012 (PDB accession of 3ARC/3WU2). A newly refined electron density map from this structure, presented here, reveals that some assignments of the cofactors may be different from those modeled in the 3ARC/3WU2 structure, including a specific Chl a that appears to have a truncated tail by one isoprene unit. We provide experimental evidence using high-performance liquid chromatography and mass spectrometry for a small population of Chl a esterified to a 15-carbon farnesyl tail in PSII of thermophilic cyanobacteria.
| Original language | English |
|---|---|
| Pages (from-to) | 175-182 |
| Number of pages | 8 |
| Journal | Photosynthesis Research |
| Volume | 134 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Nov 1 2017 |
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Keywords
- Chlorophyll a
- CP43
- Photosystem II
- Thermosynechococcus elongatus
- X-ray crystal structure
ASJC Scopus subject areas
- Biochemistry
- Plant Science
- Cell Biology
Cite this
Chlorophyll a with a farnesyl tail in thermophilic cyanobacteria. / Wiwczar, Jessica M.; LaFountain, Amy M.; Wang, Jimin; Frank, Harry A.; Brudvig, Gary W.
In: Photosynthesis Research, Vol. 134, No. 2, 01.11.2017, p. 175-182.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chlorophyll a with a farnesyl tail in thermophilic cyanobacteria
AU - Wiwczar, Jessica M.
AU - LaFountain, Amy M.
AU - Wang, Jimin
AU - Frank, Harry A.
AU - Brudvig, Gary W
PY - 2017/11/1
Y1 - 2017/11/1
N2 - Photosystem II (PSII) of oxygenic photosynthetic organisms normally contains exclusively chlorophyll a (Chl a) as its major light-harvesting pigment. Chl a canonically consists of the chlorin headgroup with a 20-carbon, 4-isoprene unit, phytyl tail. We have examined the 1.9 Å crystal structure of PSII from thermophilic cyanobacteria reported by Shen and coworkers in 2012 (PDB accession of 3ARC/3WU2). A newly refined electron density map from this structure, presented here, reveals that some assignments of the cofactors may be different from those modeled in the 3ARC/3WU2 structure, including a specific Chl a that appears to have a truncated tail by one isoprene unit. We provide experimental evidence using high-performance liquid chromatography and mass spectrometry for a small population of Chl a esterified to a 15-carbon farnesyl tail in PSII of thermophilic cyanobacteria.
AB - Photosystem II (PSII) of oxygenic photosynthetic organisms normally contains exclusively chlorophyll a (Chl a) as its major light-harvesting pigment. Chl a canonically consists of the chlorin headgroup with a 20-carbon, 4-isoprene unit, phytyl tail. We have examined the 1.9 Å crystal structure of PSII from thermophilic cyanobacteria reported by Shen and coworkers in 2012 (PDB accession of 3ARC/3WU2). A newly refined electron density map from this structure, presented here, reveals that some assignments of the cofactors may be different from those modeled in the 3ARC/3WU2 structure, including a specific Chl a that appears to have a truncated tail by one isoprene unit. We provide experimental evidence using high-performance liquid chromatography and mass spectrometry for a small population of Chl a esterified to a 15-carbon farnesyl tail in PSII of thermophilic cyanobacteria.
KW - Chlorophyll a
KW - CP43
KW - Photosystem II
KW - Thermosynechococcus elongatus
KW - X-ray crystal structure
UR - http://www.scopus.com/inward/record.url?scp=85025689722&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85025689722&partnerID=8YFLogxK
U2 - 10.1007/s11120-017-0425-4
DO - 10.1007/s11120-017-0425-4
M3 - Article
C2 - 28741056
AN - SCOPUS:85025689722
VL - 134
SP - 175
EP - 182
JO - Photosynthesis Research
JF - Photosynthesis Research
SN - 0166-8595
IS - 2
ER -