Abstract
With the advent of X-ray free electron lasers (XFELs), time-resolved serial femtosecond X-ray crystallography (TR-SFX) studies have become possible elucidating insight in protein dynamics of important processes such as photosynthesis or antibiotic resistance. A major factor limiting TR-SFX experiments are reliable injection technologies compatible with the liquid jet injection methods employed for SFX which allow fast mixing and reach suitable reaction time points. Here, we present the first 3D printed hybrid nozzle device injecting aqueous protein crystal jets after fast mixing (<5 ms), reaching reaction time points (~150 ms) suitable to resolve enzyme kinetics.
| Original language | English |
|---|---|
| Title of host publication | 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1495-1496 |
| Number of pages | 2 |
| ISBN (Electronic) | 9780692941836 |
| Publication status | Published - Jan 1 2020 |
| Event | 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States Duration: Oct 22 2017 → Oct 26 2017 |
Publication series
| Name | 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 |
|---|
Conference
| Conference | 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 |
|---|---|
| Country | United States |
| City | Savannah |
| Period | 10/22/17 → 10/26/17 |
Keywords
- 3D printing
- Mixing
- Serial femtosecond crystallography
- Time-resolved
- X-ray free electron laser
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering
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Cite this
Brehm, G., Echelmeier, A., Coe, J., Conrad, C. E., Nelson, G., Villarreal, J. C., Weierstall, U., Spence, J., Köster, S., Fromme, P., & Ros, A. (2020). Combined hydrodynamic mixer and gas dynamic virtual nozzle devices for serial femtosecond crystallography. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 (pp. 1495-1496). (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017). Chemical and Biological Microsystems Society.