TY - GEN
T1 - Combined hydrodynamic mixer and gas dynamic virtual nozzle devices for serial femtosecond crystallography
AU - Brehm, Gerrit
AU - Echelmeier, Austin
AU - Coe, Jesse
AU - Conrad, Chelsie E.
AU - Nelson, Garrett
AU - Villarreal, Jorvani Cruz
AU - Weierstall, Uwe
AU - Spence, John
AU - Köster, Sarah
AU - Fromme, Petra
AU - Ros, Alexandra
PY - 2020/1/1
Y1 - 2020/1/1
N2 - With the advent of X-ray free electron lasers (XFELs), time-resolved serial femtosecond X-ray crystallography (TR-SFX) studies have become possible elucidating insight in protein dynamics of important processes such as photosynthesis or antibiotic resistance. A major factor limiting TR-SFX experiments are reliable injection technologies compatible with the liquid jet injection methods employed for SFX which allow fast mixing and reach suitable reaction time points. Here, we present the first 3D printed hybrid nozzle device injecting aqueous protein crystal jets after fast mixing (<5 ms), reaching reaction time points (~150 ms) suitable to resolve enzyme kinetics.
AB - With the advent of X-ray free electron lasers (XFELs), time-resolved serial femtosecond X-ray crystallography (TR-SFX) studies have become possible elucidating insight in protein dynamics of important processes such as photosynthesis or antibiotic resistance. A major factor limiting TR-SFX experiments are reliable injection technologies compatible with the liquid jet injection methods employed for SFX which allow fast mixing and reach suitable reaction time points. Here, we present the first 3D printed hybrid nozzle device injecting aqueous protein crystal jets after fast mixing (<5 ms), reaching reaction time points (~150 ms) suitable to resolve enzyme kinetics.
KW - 3D printing
KW - Mixing
KW - Serial femtosecond crystallography
KW - Time-resolved
KW - X-ray free electron laser
UR - http://www.scopus.com/inward/record.url?scp=85079596997&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85079596997&partnerID=8YFLogxK
M3 - Conference contribution
T3 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
SP - 1495
EP - 1496
BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PB - Chemical and Biological Microsystems Society
T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Y2 - 22 October 2017 through 26 October 2017
ER -