Comparisons of the structural and chemical properties of melanosomes isolated from retinal pigment epithelium, iris and choroid of newborn and mature bovine eyes

Yan Liu, Lian Hong, Kazumasa Wakamatsu, Shosuke Ito, Bhavin B. Adhyaru, Chi Yuan Cheng, Clifford R. Bowers, John D. Simon

Research output: Contribution to journalArticle

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Abstract

Melanosomes were isolated from the retinal pigment epithelium (RPE), iris and choroid of mature (age >2 years) and newborn (age iris > RPE, and exhibit decreasing yields with age. 13C solid-state nuclear magnetic resonance (NMR) spectroscopic analysis of iris and choroid melanosomes indicates the same trends. These observations suggest that the 5,6-dihydroxyindole-2-carboxylic acid contents decrease in the following order: choroid > iris > RPE, and decrease with age. Moreover, the 13C solid-state NMR spectra show (1) for the same age samples, the CH:Cq ratio for choroid is larger than that for iris melanosomes; and (2) an increase in the concentration of carbonyl groups with age within each type of melanosome.

Original languageEnglish
Pages (from-to)510-516
Number of pages7
JournalPhotochemistry and Photobiology
Volume81
Issue number3
DOIs
Publication statusPublished - May 2005

Fingerprint

Melanosomes
Retinal Pigments
Choroid
epithelium
Retinal Pigment Epithelium
Iris
pigments
chemical properties
Chemical properties
Structural properties
Newborn Infant
Nuclear magnetic resonance
solid state
nuclear magnetic resonance
Spectroscopic analysis
spectroscopic analysis
carboxylic acids
Magnetic Resonance Spectroscopy
methylidyne
trends

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Comparisons of the structural and chemical properties of melanosomes isolated from retinal pigment epithelium, iris and choroid of newborn and mature bovine eyes. / Liu, Yan; Hong, Lian; Wakamatsu, Kazumasa; Ito, Shosuke; Adhyaru, Bhavin B.; Cheng, Chi Yuan; Bowers, Clifford R.; Simon, John D.

In: Photochemistry and Photobiology, Vol. 81, No. 3, 05.2005, p. 510-516.

Research output: Contribution to journalArticle

Liu, Yan ; Hong, Lian ; Wakamatsu, Kazumasa ; Ito, Shosuke ; Adhyaru, Bhavin B. ; Cheng, Chi Yuan ; Bowers, Clifford R. ; Simon, John D. / Comparisons of the structural and chemical properties of melanosomes isolated from retinal pigment epithelium, iris and choroid of newborn and mature bovine eyes. In: Photochemistry and Photobiology. 2005 ; Vol. 81, No. 3. pp. 510-516.
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