Component B binding to the soluble methane monooxygenase hydroxylase by saturation-recovery EPR spectroscopy of spin-labeled MMOB

Ryan MacArthur; Matthew H. Sazinsky; Henriette Kühne; Douglas A. Whittington; Stephen J. Lippard; Gary W Brudvig

doi:10.1021/ja0279904

Component B binding to the soluble methane monooxygenase hydroxylase by saturation-recovery EPR spectroscopy of spin-labeled MMOB

Ryan MacArthur, Matthew H. Sazinsky, Henriette Kühne, Douglas A. Whittington, Stephen J. Lippard, Gary W Brudvig

Yale University

Research output: Contribution to journal › Article

15 Citations (Scopus)

Abstract

Spin-labeled Cys89 of the soluble methane monooxygenase regulatory protein (MMOB) from Methylococcus capsulatus (Bath) binds within 15 ± 4 Å of the hydroxylase (MMOH) diiron center, placing the MMOB docking site in the MMOH "canyon" region on iron-coordinating helices E and F of the α-subunit.

Original language	English
Pages (from-to)	13392-13393
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	124
Issue number	45
DOIs	https://doi.org/10.1021/ja0279904
Publication status	Published - Nov 13 2002

Fingerprint

methane monooxygenase

Methylococcus capsulatus

Mixed Function Oxygenases

Baths

Paramagnetic resonance

Spectrum Analysis

Methane

Iron

Spectroscopy

Proteins

Recovery

ASJC Scopus subject areas

Chemistry(all)

Cite this

MacArthur, R., Sazinsky, M. H., Kühne, H., Whittington, D. A., Lippard, S. J., & Brudvig, G. W. (2002). Component B binding to the soluble methane monooxygenase hydroxylase by saturation-recovery EPR spectroscopy of spin-labeled MMOB. Journal of the American Chemical Society, 124(45), 13392-13393. https://doi.org/10.1021/ja0279904

Component B binding to the soluble methane monooxygenase hydroxylase by saturation-recovery EPR spectroscopy of spin-labeled MMOB. / MacArthur, Ryan; Sazinsky, Matthew H.; Kühne, Henriette; Whittington, Douglas A.; Lippard, Stephen J.; Brudvig, Gary W.

In: Journal of the American Chemical Society, Vol. 124, No. 45, 13.11.2002, p. 13392-13393.

Research output: Contribution to journal › Article

MacArthur, R, Sazinsky, MH, Kühne, H, Whittington, DA, Lippard, SJ & Brudvig, GW 2002, 'Component B binding to the soluble methane monooxygenase hydroxylase by saturation-recovery EPR spectroscopy of spin-labeled MMOB', Journal of the American Chemical Society, vol. 124, no. 45, pp. 13392-13393. https://doi.org/10.1021/ja0279904

MacArthur R, Sazinsky MH, Kühne H, Whittington DA, Lippard SJ, Brudvig GW. Component B binding to the soluble methane monooxygenase hydroxylase by saturation-recovery EPR spectroscopy of spin-labeled MMOB. Journal of the American Chemical Society. 2002 Nov 13;124(45):13392-13393. https://doi.org/10.1021/ja0279904

MacArthur, Ryan ; Sazinsky, Matthew H. ; Kühne, Henriette ; Whittington, Douglas A. ; Lippard, Stephen J. ; Brudvig, Gary W. / Component B binding to the soluble methane monooxygenase hydroxylase by saturation-recovery EPR spectroscopy of spin-labeled MMOB. In: Journal of the American Chemical Society. 2002 ; Vol. 124, No. 45. pp. 13392-13393.

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10.1021/ja0279904