Component B binding to the soluble methane monooxygenase hydroxylase by saturation-recovery EPR spectroscopy of spin-labeled MMOB

Ryan MacArthur; Matthew H. Sazinsky; Henriette Kühne; Douglas A. Whittington; Stephen J. Lippard; Gary W Brudvig

doi:10.1021/ja0279904

Component B binding to the soluble methane monooxygenase hydroxylase by saturation-recovery EPR spectroscopy of spin-labeled MMOB

Ryan MacArthur, Matthew H. Sazinsky, Henriette Kühne, Douglas A. Whittington, Stephen J. Lippard, Gary W Brudvig

Yale University

Research output: Contribution to journal › Article

16 Citations (Scopus)

Abstract

Spin-labeled Cys89 of the soluble methane monooxygenase regulatory protein (MMOB) from Methylococcus capsulatus (Bath) binds within 15 ± 4 Å of the hydroxylase (MMOH) diiron center, placing the MMOB docking site in the MMOH "canyon" region on iron-coordinating helices E and F of the α-subunit.

Original language	English
Pages (from-to)	13392-13393
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	124
Issue number	45
DOIs	https://doi.org/10.1021/ja0279904
Publication status	Published - Nov 13 2002

Fingerprint

methane monooxygenase

Methylococcus capsulatus

Mixed Function Oxygenases

Baths

Paramagnetic resonance

Spectrum Analysis

Methane

Iron

Spectroscopy

Proteins

Recovery

ASJC Scopus subject areas

Chemistry(all)

Cite this

MacArthur, R., Sazinsky, M. H., Kühne, H., Whittington, D. A., Lippard, S. J., & Brudvig, G. W. (2002). Component B binding to the soluble methane monooxygenase hydroxylase by saturation-recovery EPR spectroscopy of spin-labeled MMOB. Journal of the American Chemical Society, 124(45), 13392-13393. https://doi.org/10.1021/ja0279904

Component B binding to the soluble methane monooxygenase hydroxylase by saturation-recovery EPR spectroscopy of spin-labeled MMOB. / MacArthur, Ryan; Sazinsky, Matthew H.; Kühne, Henriette; Whittington, Douglas A.; Lippard, Stephen J.; Brudvig, Gary W.

In: Journal of the American Chemical Society, Vol. 124, No. 45, 13.11.2002, p. 13392-13393.

Research output: Contribution to journal › Article

MacArthur, R, Sazinsky, MH, Kühne, H, Whittington, DA, Lippard, SJ & Brudvig, GW 2002, 'Component B binding to the soluble methane monooxygenase hydroxylase by saturation-recovery EPR spectroscopy of spin-labeled MMOB', Journal of the American Chemical Society, vol. 124, no. 45, pp. 13392-13393. https://doi.org/10.1021/ja0279904

MacArthur R, Sazinsky MH, Kühne H, Whittington DA, Lippard SJ, Brudvig GW. Component B binding to the soluble methane monooxygenase hydroxylase by saturation-recovery EPR spectroscopy of spin-labeled MMOB. Journal of the American Chemical Society. 2002 Nov 13;124(45):13392-13393. https://doi.org/10.1021/ja0279904

MacArthur, Ryan ; Sazinsky, Matthew H. ; Kühne, Henriette ; Whittington, Douglas A. ; Lippard, Stephen J. ; Brudvig, Gary W. / Component B binding to the soluble methane monooxygenase hydroxylase by saturation-recovery EPR spectroscopy of spin-labeled MMOB. In: Journal of the American Chemical Society. 2002 ; Vol. 124, No. 45. pp. 13392-13393.

@article{f459e67f1c6a4b8c83851e4580e34b29,

title = "Component B binding to the soluble methane monooxygenase hydroxylase by saturation-recovery EPR spectroscopy of spin-labeled MMOB",

abstract = "Spin-labeled Cys89 of the soluble methane monooxygenase regulatory protein (MMOB) from Methylococcus capsulatus (Bath) binds within 15 ± 4 {\AA} of the hydroxylase (MMOH) diiron center, placing the MMOB docking site in the MMOH {"}canyon{"} region on iron-coordinating helices E and F of the α-subunit.",

author = "Ryan MacArthur and Sazinsky, {Matthew H.} and Henriette K{\"u}hne and Whittington, {Douglas A.} and Lippard, {Stephen J.} and Brudvig, {Gary W}",

year = "2002",

month = "11",

day = "13",

doi = "10.1021/ja0279904",

language = "English",

volume = "124",

pages = "13392--13393",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

number = "45",

}

TY - JOUR

T1 - Component B binding to the soluble methane monooxygenase hydroxylase by saturation-recovery EPR spectroscopy of spin-labeled MMOB

AU - MacArthur, Ryan

AU - Sazinsky, Matthew H.

AU - Kühne, Henriette

AU - Whittington, Douglas A.

AU - Lippard, Stephen J.

AU - Brudvig, Gary W

PY - 2002/11/13

Y1 - 2002/11/13

N2 - Spin-labeled Cys89 of the soluble methane monooxygenase regulatory protein (MMOB) from Methylococcus capsulatus (Bath) binds within 15 ± 4 Å of the hydroxylase (MMOH) diiron center, placing the MMOB docking site in the MMOH "canyon" region on iron-coordinating helices E and F of the α-subunit.

AB - Spin-labeled Cys89 of the soluble methane monooxygenase regulatory protein (MMOB) from Methylococcus capsulatus (Bath) binds within 15 ± 4 Å of the hydroxylase (MMOH) diiron center, placing the MMOB docking site in the MMOH "canyon" region on iron-coordinating helices E and F of the α-subunit.

UR - http://www.scopus.com/inward/record.url?scp=0037073201&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037073201&partnerID=8YFLogxK

U2 - 10.1021/ja0279904

DO - 10.1021/ja0279904

M3 - Article

C2 - 12418885

AN - SCOPUS:0037073201

VL - 124

SP - 13392

EP - 13393

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 45

ER -

Access to Document

10.1021/ja0279904