Component B binding to the soluble methane monooxygenase hydroxylase by saturation-recovery EPR spectroscopy of spin-labeled MMOB

Ryan MacArthur, Matthew H. Sazinsky, Henriette Kühne, Douglas A. Whittington, Stephen J. Lippard, Gary W. Brudvig

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Abstract

Spin-labeled Cys89 of the soluble methane monooxygenase regulatory protein (MMOB) from Methylococcus capsulatus (Bath) binds within 15 ± 4 Å of the hydroxylase (MMOH) diiron center, placing the MMOB docking site in the MMOH "canyon" region on iron-coordinating helices E and F of the α-subunit.

Original languageEnglish
Pages (from-to)13392-13393
Number of pages2
JournalJournal of the American Chemical Society
Volume124
Issue number45
DOIs
Publication statusPublished - Nov 13 2002

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ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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