Abstract
Using a pulsed UV laser in a confocal scanning microscope, we present a relatively cheap, accurate and efficient method for fast UV laser flash photolysis of caged molecules in two-dimensional cultured neurons. The laser light is introduced through the imaging optics, can be localized by a parallel red laser and can photolyse a sphere of less than 1μm2, and evoke local fluorescence changes in the imaged neurons. Caged glutamate and caged fluorescein are used to illustrate a disparity between spines and their parent dendrites at a sub-micron resolution.
| Original language | English |
|---|---|
| Pages (from-to) | 153-159 |
| Number of pages | 7 |
| Journal | Journal of Neuroscience Methods |
| Volume | 133 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Mar 15 2004 |
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Keywords
- Confocal microscope
- Culture
- Glutamate
- Hippocampus
- Uncaging
- UV laser
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Confocal microscopic imaging of fast UV-laser photolysis of caged compounds. / Korkotian, Eduard; Oron, Dan; Silberberg, Yaron; Segal, Menahem.
In: Journal of Neuroscience Methods, Vol. 133, No. 1-2, 15.03.2004, p. 153-159.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Confocal microscopic imaging of fast UV-laser photolysis of caged compounds
AU - Korkotian, Eduard
AU - Oron, Dan
AU - Silberberg, Yaron
AU - Segal, Menahem
PY - 2004/3/15
Y1 - 2004/3/15
N2 - Using a pulsed UV laser in a confocal scanning microscope, we present a relatively cheap, accurate and efficient method for fast UV laser flash photolysis of caged molecules in two-dimensional cultured neurons. The laser light is introduced through the imaging optics, can be localized by a parallel red laser and can photolyse a sphere of less than 1μm2, and evoke local fluorescence changes in the imaged neurons. Caged glutamate and caged fluorescein are used to illustrate a disparity between spines and their parent dendrites at a sub-micron resolution.
AB - Using a pulsed UV laser in a confocal scanning microscope, we present a relatively cheap, accurate and efficient method for fast UV laser flash photolysis of caged molecules in two-dimensional cultured neurons. The laser light is introduced through the imaging optics, can be localized by a parallel red laser and can photolyse a sphere of less than 1μm2, and evoke local fluorescence changes in the imaged neurons. Caged glutamate and caged fluorescein are used to illustrate a disparity between spines and their parent dendrites at a sub-micron resolution.
KW - Confocal microscope
KW - Culture
KW - Glutamate
KW - Hippocampus
KW - Uncaging
KW - UV laser
UR - http://www.scopus.com/inward/record.url?scp=0742324459&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0742324459&partnerID=8YFLogxK
U2 - 10.1016/j.jneumeth.2003.10.007
DO - 10.1016/j.jneumeth.2003.10.007
M3 - Article
C2 - 14757356
AN - SCOPUS:0742324459
VL - 133
SP - 153
EP - 159
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
SN - 0165-0270
IS - 1-2
ER -