Confocal microscopic imaging of fast UV-laser photolysis of caged compounds

Eduard Korkotian, Dan Oron, Yaron Silberberg, Menahem Segal

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Using a pulsed UV laser in a confocal scanning microscope, we present a relatively cheap, accurate and efficient method for fast UV laser flash photolysis of caged molecules in two-dimensional cultured neurons. The laser light is introduced through the imaging optics, can be localized by a parallel red laser and can photolyse a sphere of less than 1μm2, and evoke local fluorescence changes in the imaged neurons. Caged glutamate and caged fluorescein are used to illustrate a disparity between spines and their parent dendrites at a sub-micron resolution.

Original languageEnglish
Pages (from-to)153-159
Number of pages7
JournalJournal of Neuroscience Methods
Volume133
Issue number1-2
DOIs
Publication statusPublished - Mar 15 2004

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Keywords

  • Confocal microscope
  • Culture
  • Glutamate
  • Hippocampus
  • UV laser
  • Uncaging

ASJC Scopus subject areas

  • Neuroscience(all)

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