Abstract
Using a pulsed UV laser in a confocal scanning microscope, we present a relatively cheap, accurate and efficient method for fast UV laser flash photolysis of caged molecules in two-dimensional cultured neurons. The laser light is introduced through the imaging optics, can be localized by a parallel red laser and can photolyse a sphere of less than 1μm2, and evoke local fluorescence changes in the imaged neurons. Caged glutamate and caged fluorescein are used to illustrate a disparity between spines and their parent dendrites at a sub-micron resolution.
| Original language | English |
|---|---|
| Pages (from-to) | 153-159 |
| Number of pages | 7 |
| Journal | Journal of Neuroscience Methods |
| Volume | 133 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Mar 15 2004 |
Keywords
- Confocal microscope
- Culture
- Glutamate
- Hippocampus
- UV laser
- Uncaging
ASJC Scopus subject areas
- Neuroscience(all)
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Korkotian, E., Oron, D., Silberberg, Y., & Segal, M. (2004). Confocal microscopic imaging of fast UV-laser photolysis of caged compounds. Journal of Neuroscience Methods, 133(1-2), 153-159. https://doi.org/10.1016/j.jneumeth.2003.10.007