Confocal microscopic imaging of fast UV-laser photolysis of caged compounds

Eduard Korkotian, Dan Oron, Yaron Silberberg, Menahem Segal

Research output: Contribution to journal › Article

16 Citations (Scopus)

Abstract

Using a pulsed UV laser in a confocal scanning microscope, we present a relatively cheap, accurate and efficient method for fast UV laser flash photolysis of caged molecules in two-dimensional cultured neurons. The laser light is introduced through the imaging optics, can be localized by a parallel red laser and can photolyse a sphere of less than 1μm2, and evoke local fluorescence changes in the imaged neurons. Caged glutamate and caged fluorescein are used to illustrate a disparity between spines and their parent dendrites at a sub-micron resolution.

Original languageEnglish
Pages (from-to)153-159
Number of pages7
JournalJournal of Neuroscience Methods
Volume133
Issue number1-2
DOIs
Publication statusPublished - Mar 15 2004

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Photolysis
Lasers
Neurons
Dendrites
Fluorescein
Glutamic Acid
Spine
Fluorescence
Light

Keywords

  • Confocal microscope
  • Culture
  • Glutamate
  • Hippocampus
  • Uncaging
  • UV laser

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Confocal microscopic imaging of fast UV-laser photolysis of caged compounds. / Korkotian, Eduard; Oron, Dan; Silberberg, Yaron; Segal, Menahem.

In: Journal of Neuroscience Methods, Vol. 133, No. 1-2, 15.03.2004, p. 153-159.

Research output: Contribution to journal › Article

Korkotian, E, Oron, D, Silberberg, Y & Segal, M 2004, 'Confocal microscopic imaging of fast UV-laser photolysis of caged compounds', Journal of Neuroscience Methods, vol. 133, no. 1-2, pp. 153-159. https://doi.org/10.1016/j.jneumeth.2003.10.007
Korkotian E, Oron D, Silberberg Y, Segal M. Confocal microscopic imaging of fast UV-laser photolysis of caged compounds. Journal of Neuroscience Methods. 2004 Mar 15;133(1-2):153-159. https://doi.org/10.1016/j.jneumeth.2003.10.007
Korkotian, Eduard ; Oron, Dan ; Silberberg, Yaron ; Segal, Menahem. / Confocal microscopic imaging of fast UV-laser photolysis of caged compounds. In: Journal of Neuroscience Methods. 2004 ; Vol. 133, No. 1-2. pp. 153-159.
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