Construction of lac fusions to the inducible arginine- and lysine decarboxylase genes of Escherichia coli K12

E. A. Auger, K. E. Redding, T. Plumb, L. C. Childs, S. Y. Meng, G. N. Bennett

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The induction of several amino acid decarboxylases under anaerobic conditions at low pH has been known for many years, but the mechanism associated with this type of regulation has not been elucidated. To study the regulation of the biodegradative arginine and lysine decarboxylases of Escherichia coli K12, Mudlac fusions to these genes were isolated. Mudlac fusion strains deficient for lysine decarboxylase or arginine decarboxylase were identified using decarboxylase indicator media and analysed for their regulation of β-galactosidase expression. The position of the Mudlac fusion in lysine decarboxylase-deficient strains has been mapped to the cadA gene at 93.7 minutes, while the Mudlac fusions exhibiting a deficiency in the inducible arginine decarboxylase have been mapped to 93.4 minutes.

Original languageEnglish
Pages (from-to)609-620
Number of pages12
JournalMolecular Microbiology
Volume3
Issue number5
Publication statusPublished - 1989

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lysine decarboxylase
Escherichia coli K12
Carboxy-Lyases
Galactosidases
Genes
Gene Fusion
Amino Acids
arginine decarboxylase

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Construction of lac fusions to the inducible arginine- and lysine decarboxylase genes of Escherichia coli K12. / Auger, E. A.; Redding, K. E.; Plumb, T.; Childs, L. C.; Meng, S. Y.; Bennett, G. N.

In: Molecular Microbiology, Vol. 3, No. 5, 1989, p. 609-620.

Research output: Contribution to journalArticle

Auger, E. A. ; Redding, K. E. ; Plumb, T. ; Childs, L. C. ; Meng, S. Y. ; Bennett, G. N. / Construction of lac fusions to the inducible arginine- and lysine decarboxylase genes of Escherichia coli K12. In: Molecular Microbiology. 1989 ; Vol. 3, No. 5. pp. 609-620.
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