Controlling cell growth with tailorable 2D nanoholes arrays

Vanessa H. Fragal, Thelma Sley P Cellet, Elizângela H. Fragal, Guilherme M. Pereira, Francielle P. Garcia, Celso V. Nakamura, Teddy Asefa, Adley F. Rubira, Rafael Silva

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A facile and reproducible route that can lead to two-dimensional arrays of nanopores in thin polymer films is demonstrated. The formation of the pores in the polymer films involves breath figure phenomenon and occurs during the film deposition by spin coating. The formation of nanoporous thin films takes only few seconds, and the method does not require complex equipment or expensive chemicals. This method also constitutes a straightforward approach to control the size of the pores formed in thin films. Besides allowing control over the average pore size of the porous films, the use of dynamic deposition with the breath figure phenomenon causes the reduction in the pore size to nanometer scale. The nanoporous arrays obtained by the breath figure are applied as substrates for cell growth, and the effect of their nanopore size on cell growth was evaluated. Notably, it is found that cell viability is related to pore size, where 2D nanoporous structure is more beneficial for cell culture than 2D microporous structures. The change in the average pore size of the polymer films from 1.22 μm to 346 nm results in a threefold increase in cell viability.

Original languageEnglish
Pages (from-to)150-161
Number of pages12
JournalJournal of Colloid and Interface Science
Volume466
DOIs
Publication statusPublished - Mar 15 2016

Fingerprint

Cell growth
Pore size
Polymer films
Nanopores
Thin films
Cells
Spin coating
Cell culture
Substrates

Keywords

  • Breath figure method
  • Cell growth
  • Nanofabrication
  • Nanopore arrays
  • Polystyrene

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Colloid and Surface Chemistry

Cite this

Fragal, V. H., Cellet, T. S. P., Fragal, E. H., Pereira, G. M., Garcia, F. P., Nakamura, C. V., ... Silva, R. (2016). Controlling cell growth with tailorable 2D nanoholes arrays. Journal of Colloid and Interface Science, 466, 150-161. https://doi.org/10.1016/j.jcis.2015.12.016

Controlling cell growth with tailorable 2D nanoholes arrays. / Fragal, Vanessa H.; Cellet, Thelma Sley P; Fragal, Elizângela H.; Pereira, Guilherme M.; Garcia, Francielle P.; Nakamura, Celso V.; Asefa, Teddy; Rubira, Adley F.; Silva, Rafael.

In: Journal of Colloid and Interface Science, Vol. 466, 15.03.2016, p. 150-161.

Research output: Contribution to journalArticle

Fragal, VH, Cellet, TSP, Fragal, EH, Pereira, GM, Garcia, FP, Nakamura, CV, Asefa, T, Rubira, AF & Silva, R 2016, 'Controlling cell growth with tailorable 2D nanoholes arrays', Journal of Colloid and Interface Science, vol. 466, pp. 150-161. https://doi.org/10.1016/j.jcis.2015.12.016
Fragal VH, Cellet TSP, Fragal EH, Pereira GM, Garcia FP, Nakamura CV et al. Controlling cell growth with tailorable 2D nanoholes arrays. Journal of Colloid and Interface Science. 2016 Mar 15;466:150-161. https://doi.org/10.1016/j.jcis.2015.12.016
Fragal, Vanessa H. ; Cellet, Thelma Sley P ; Fragal, Elizângela H. ; Pereira, Guilherme M. ; Garcia, Francielle P. ; Nakamura, Celso V. ; Asefa, Teddy ; Rubira, Adley F. ; Silva, Rafael. / Controlling cell growth with tailorable 2D nanoholes arrays. In: Journal of Colloid and Interface Science. 2016 ; Vol. 466. pp. 150-161.
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