TY - JOUR
T1 - Controlling cell growth with tailorable 2D nanoholes arrays
AU - Fragal, Vanessa H.
AU - Cellet, Thelma Sley P.
AU - Fragal, Elizângela H.
AU - Pereira, Guilherme M.
AU - Garcia, Francielle P.
AU - Nakamura, Celso V.
AU - Asefa, Tewodros
AU - Rubira, Adley F.
AU - Silva, Rafael
N1 - Funding Information:
VHF and EHF thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES–Brazil) for doctorate fellowships. T.S.P.C thanks the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq–Brazil) for doctorate fellowships. A.F.R acknowledges the financial supports given by CNPq , Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES–Brazil) and Fundação Araucária—Brazil . TA gratefully acknowledges the financial support of the Rutgers Center for Global Advancement and International Affairs (GAIA) program and the CNPq Science Without Borders Fellowships for Special Visiting Professorship in Brazil.
PY - 2016/3/15
Y1 - 2016/3/15
N2 - A facile and reproducible route that can lead to two-dimensional arrays of nanopores in thin polymer films is demonstrated. The formation of the pores in the polymer films involves breath figure phenomenon and occurs during the film deposition by spin coating. The formation of nanoporous thin films takes only few seconds, and the method does not require complex equipment or expensive chemicals. This method also constitutes a straightforward approach to control the size of the pores formed in thin films. Besides allowing control over the average pore size of the porous films, the use of dynamic deposition with the breath figure phenomenon causes the reduction in the pore size to nanometer scale. The nanoporous arrays obtained by the breath figure are applied as substrates for cell growth, and the effect of their nanopore size on cell growth was evaluated. Notably, it is found that cell viability is related to pore size, where 2D nanoporous structure is more beneficial for cell culture than 2D microporous structures. The change in the average pore size of the polymer films from 1.22 μm to 346 nm results in a threefold increase in cell viability.
AB - A facile and reproducible route that can lead to two-dimensional arrays of nanopores in thin polymer films is demonstrated. The formation of the pores in the polymer films involves breath figure phenomenon and occurs during the film deposition by spin coating. The formation of nanoporous thin films takes only few seconds, and the method does not require complex equipment or expensive chemicals. This method also constitutes a straightforward approach to control the size of the pores formed in thin films. Besides allowing control over the average pore size of the porous films, the use of dynamic deposition with the breath figure phenomenon causes the reduction in the pore size to nanometer scale. The nanoporous arrays obtained by the breath figure are applied as substrates for cell growth, and the effect of their nanopore size on cell growth was evaluated. Notably, it is found that cell viability is related to pore size, where 2D nanoporous structure is more beneficial for cell culture than 2D microporous structures. The change in the average pore size of the polymer films from 1.22 μm to 346 nm results in a threefold increase in cell viability.
KW - Breath figure method
KW - Cell growth
KW - Nanofabrication
KW - Nanopore arrays
KW - Polystyrene
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U2 - 10.1016/j.jcis.2015.12.016
DO - 10.1016/j.jcis.2015.12.016
M3 - Article
C2 - 26722796
AN - SCOPUS:84951172227
VL - 466
SP - 150
EP - 161
JO - Journal of Colloid and Interface Science
JF - Journal of Colloid and Interface Science
SN - 0021-9797
ER -