Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses

Ruslan P. Kurta, Jeffrey J. Donatelli, Chun Hong Yoon, Peter Berntsen, Johan Bielecki, Benedikt J. Daurer, Hasan Demirci, Petra Fromme, Max Felix Hantke, Filipe R.N.C. Maia, Anna Munke, Carl Nettelblad, Kanupriya Pande, Hemanth K.N. Reddy, Jonas A. Sellberg, Raymond G. Sierra, Martin Svenda, Gijs Van Der Schot, Ivan A. Vartanyants, Garth J. WilliamsP. Lourdu Xavier, Andrew Aquila, Peter H. Zwart, Adrian P. Mancuso

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We use extremely bright and ultrashort pulses from an x-ray free-electron laser (XFEL) to measure correlations in x rays scattered from individual bioparticles. This allows us to go beyond the traditional crystallography and single-particle imaging approaches for structure investigations. We employ angular correlations to recover the three-dimensional (3D) structure of nanoscale viruses from x-ray diffraction data measured at the Linac Coherent Light Source. Correlations provide us with a comprehensive structural fingerprint of a 3D virus, which we use both for model-based and ab initio structure recovery. The analyses reveal a clear indication that the structure of the viruses deviates from the expected perfect icosahedral symmetry. Our results anticipate exciting opportunities for XFEL studies of the structure and dynamics of nanoscale objects by means of angular correlations.

Original languageEnglish
Article number158102
JournalPhysical Review Letters
Volume119
Issue number15
DOIs
Publication statusPublished - Oct 12 2017

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viruses
x ray lasers
angular correlation
free electron lasers
pulses
lasers
x rays
coherent light
crystallography
light sources
indication
x ray diffraction
recovery
symmetry

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Kurta, R. P., Donatelli, J. J., Yoon, C. H., Berntsen, P., Bielecki, J., Daurer, B. J., ... Mancuso, A. P. (2017). Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses. Physical Review Letters, 119(15), [158102]. https://doi.org/10.1103/PhysRevLett.119.158102

Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses. / Kurta, Ruslan P.; Donatelli, Jeffrey J.; Yoon, Chun Hong; Berntsen, Peter; Bielecki, Johan; Daurer, Benedikt J.; Demirci, Hasan; Fromme, Petra; Hantke, Max Felix; Maia, Filipe R.N.C.; Munke, Anna; Nettelblad, Carl; Pande, Kanupriya; Reddy, Hemanth K.N.; Sellberg, Jonas A.; Sierra, Raymond G.; Svenda, Martin; Van Der Schot, Gijs; Vartanyants, Ivan A.; Williams, Garth J.; Xavier, P. Lourdu; Aquila, Andrew; Zwart, Peter H.; Mancuso, Adrian P.

In: Physical Review Letters, Vol. 119, No. 15, 158102, 12.10.2017.

Research output: Contribution to journalArticle

Kurta, RP, Donatelli, JJ, Yoon, CH, Berntsen, P, Bielecki, J, Daurer, BJ, Demirci, H, Fromme, P, Hantke, MF, Maia, FRNC, Munke, A, Nettelblad, C, Pande, K, Reddy, HKN, Sellberg, JA, Sierra, RG, Svenda, M, Van Der Schot, G, Vartanyants, IA, Williams, GJ, Xavier, PL, Aquila, A, Zwart, PH & Mancuso, AP 2017, 'Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses', Physical Review Letters, vol. 119, no. 15, 158102. https://doi.org/10.1103/PhysRevLett.119.158102
Kurta, Ruslan P. ; Donatelli, Jeffrey J. ; Yoon, Chun Hong ; Berntsen, Peter ; Bielecki, Johan ; Daurer, Benedikt J. ; Demirci, Hasan ; Fromme, Petra ; Hantke, Max Felix ; Maia, Filipe R.N.C. ; Munke, Anna ; Nettelblad, Carl ; Pande, Kanupriya ; Reddy, Hemanth K.N. ; Sellberg, Jonas A. ; Sierra, Raymond G. ; Svenda, Martin ; Van Der Schot, Gijs ; Vartanyants, Ivan A. ; Williams, Garth J. ; Xavier, P. Lourdu ; Aquila, Andrew ; Zwart, Peter H. ; Mancuso, Adrian P. / Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses. In: Physical Review Letters. 2017 ; Vol. 119, No. 15.
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