Crossing the Thauer limit

Rewiring cyanobacterial metabolism to maximize fermentative H 2 production

Kenchappa G. Kumaraswamy, Anagha Krishnan, Gennady Ananyev, Shuyi Zhang, Donald A. Bryant, G Charles Dismukes

Research output: Contribution to journalArticle

Abstract

Many cyanobacteria power metabolism during dark anaerobic conditions by the catabolism of glycogen which creates adenylate energy (ATP) and NAD(P)H. The latter can be reoxidized by a reversible NiFe-hydrogenase functioning as a terminal oxidoreductase generating H 2 as byproduct. Theoretically, one glucose molecule can yield up to 12 molecules of H 2 , although this never happens in vivo. The thermodynamic preference is for glucose catabolism via the Embden-Meyerhof-Parnas (EMP) pathway (henceforth, glycolysis) which restricts the pathway yield below 4 mole H 2 per mole glucose (so-called Thauer limit). An alternate route that is not used is the oxidative pentose phosphate shunt (OPP), which theoretically can yield 3-fold more NAD(P)H than glycolysis. Herein, we engineer the cyanobacterium Synechococcus sp. PCC 7002 to redirect glycogen catabolic flux through OPP by deleting the gap1 gene for glyceraldehyde-3-phosphate dehydrogenase (GAPDH-1) and stack this with a knock-out mutation of NADH-consuming lactate dehydrogenase (ldhA). The resulting Δgap1ΔldhA double mutant when combined with the elimination of H 2 uptake by continuous electrochemical removal of H 2 was able to produce 681 μmol H 2 per g DW per day, equivalent to 6.4 mole H 2 per mole glucose, well beyond the Thauer limit. This achieves the highest in vivo autofermentative H 2 production yield of any bacterium, equivalent to 80% of the theoretical maximum of 8 H 2 per glucose via OPP, using only photoautotrophically generated glycogen as precursor with full retention of cellular viability. These findings demonstrate the plasticity of central carbon metabolism and the significant potential of metabolic engineering for redirecting carbohydrate catabolism towards hydrogen production in cyanobacteria.

Original languageEnglish
Pages (from-to)1035-1045
Number of pages11
JournalEnergy and Environmental Science
Volume12
Issue number3
DOIs
Publication statusPublished - Mar 1 2019

Fingerprint

Metabolism
Glucose
glucose
Pentoses
metabolism
catabolism
Phosphates
Glycogen
phosphate
NAD
cyanobacterium
Metabolic engineering
Glyceraldehyde-3-Phosphate Dehydrogenases
Molecules
Adenosinetriphosphate
Carbohydrates
Hydrogen production
L-Lactate Dehydrogenase
anoxic conditions
Plasticity

ASJC Scopus subject areas

  • Environmental Chemistry
  • Renewable Energy, Sustainability and the Environment
  • Nuclear Energy and Engineering
  • Pollution

Cite this

Crossing the Thauer limit : Rewiring cyanobacterial metabolism to maximize fermentative H 2 production. / Kumaraswamy, Kenchappa G.; Krishnan, Anagha; Ananyev, Gennady; Zhang, Shuyi; Bryant, Donald A.; Dismukes, G Charles.

In: Energy and Environmental Science, Vol. 12, No. 3, 01.03.2019, p. 1035-1045.

Research output: Contribution to journalArticle

Kumaraswamy, Kenchappa G. ; Krishnan, Anagha ; Ananyev, Gennady ; Zhang, Shuyi ; Bryant, Donald A. ; Dismukes, G Charles. / Crossing the Thauer limit : Rewiring cyanobacterial metabolism to maximize fermentative H 2 production. In: Energy and Environmental Science. 2019 ; Vol. 12, No. 3. pp. 1035-1045.
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