@article{d1ad2467604c48d0b51c0fd0cdf3f208,
title = "Cryo-EM Structure of Monomeric Photosystem II from Synechocystis sp. PCC 6803 Lacking the Water-Oxidation Complex",
abstract = "Solar energy is converted to chemical energy by light-driven water oxidation catalyzed by photosystem II in photosynthetic organisms. Photosystem II requires a complex system of assembly and repair mechanisms to maintain activity and, thus, is found in a variety of precursor states that are not well understood. Here, a cryo-EM structure of photosystem II lacking extrinsic and peripheral subunits, and the cofactor required for water oxidation, is reported. The structure provides insights into the mechanism of photoactivation of photosystem II.",
keywords = "high-affinity binding site, oxygen-evolving complex, photoactivation, photoassembly, photosynthesis, photosystem II, water splitting, water-oxidation complex",
author = "Gisriel, {Christopher J.} and Kaifeng Zhou and Huang, {Hao Li} and Debus, {Richard J.} and Yong Xiong and Brudvig, {Gary W.}",
note = "Funding Information: We acknowledge the Science Hill Cryo-EM Lab and Yale Cryo-EM Resource for sample screening and high-resolution data collection, respectively. We also acknowledge the Office of the Dean at the Yale School of Medicine and Office of the Provost at Yale University for funding of the Yale Cryo-EM Resource. We acknowledge Dr. Shenping Wu for her assistance in data collection on the Titan Krios at the Yale Cryo-EM Resource laboratory. We thank the Yale Center for Research Computing for guidance and use of the research computing infrastructure, especially Dr. Michael Strickler in his direction in appropriate resource usage and software setup. Figure S1 C was designed using UCSF Chimera 93 , developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco, with support from NIH P41-GM103311 . Funding Information: We acknowledge the Science Hill Cryo-EM Lab and Yale Cryo-EM Resource for sample screening and high-resolution data collection, respectively. We also acknowledge the Office of the Dean at the Yale School of Medicine and Office of the Provost at Yale University for funding of the Yale Cryo-EM Resource. We acknowledge Dr. Shenping Wu for her assistance in data collection on the Titan Krios at the Yale Cryo-EM Resource laboratory. We thank the Yale Center for Research Computing for guidance and use of the research computing infrastructure, especially Dr. Michael Strickler in his direction in appropriate resource usage and software setup. Figure S1C was designed using UCSF Chimera93, developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco, with support from NIH P41-GM103311. This work was supported by Department of Energy, Office of Basic Energy Sciences, Division of Chemical Sciences grant DE-FG02-05ER15646 to G.W.B. and the Forest B.H. and Elizabeth D.W. Brown Postdoctoral Fellowship for Plant Science to C.J.G. Conceptualization, G.W.B. and C.J.G.; Methodology, C.J.G. and K.Z.; Investigation, C.J.G. K.Z. H.-L.H. and R.J.D.; Writing? Original Draft, C.J.G.; Writing ? Review & Editing, C.J.G. K.Z. H.-L.H. R.J.D. Y.X. and G.W.B.; Visualization, C.G.; Funding Acquisition, G.W.B. Y.X. and C.J.G.; Resources, C.J.G. K.F. and Y.X.; Supervision, G.W.B. and Y.X. The authors declare no competing interests. Funding Information: This work was supported by Department of Energy , Office of Basic Energy Sciences , Division of Chemical Sciences grant DE-FG02-05ER15646 to G.W.B., and the Forest B.H. and Elizabeth D.W. Brown Postdoctoral Fellowship for Plant Science to C.J.G. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = oct,
day = "14",
doi = "10.1016/j.joule.2020.07.016",
language = "English",
volume = "4",
pages = "2131--2148",
journal = "Joule",
issn = "2542-4351",
publisher = "Cell Press",
number = "10",
}