Crystal structure of photosystem II from Synechococcus elongatus at 3.8 Å resolution

Athina Zouni, Horst Tobias Witt, Jan Kern, Petra Fromme, Norbert Krauss, Wolfram Saenger, Peter Orth

Research output: Contribution to journalArticle

1630 Citations (Scopus)

Abstract

Oxygenic photosynthesis is the principal energy converter on earth. It is driven by photosystems I and II, two large protein-cofactor complexes located in the thylakoid membrane and acting in series. In photosystem II, water is oxidized; this event provides the overall process with the necessary electrons and protons, and the atmosphere with oxygen. To date, structural information on the architecture of the complex has been provided by electron microscopy of intact, active photosystem II at 15-30 Å resolution1, and by electron crystallography on two-dimensional crystals of D1-D2-CP47 photosystem II fragments without water oxidizing activity at 8 Å resolution. Here we describe the X-ray structure of photosystem II on the basis of crystals fully active in water oxidation3. The structure shows how protein subunits and cofactors are spatially organized. The larger subunits are assigned and the locations and orientations of the cofactors are defined. We also provide new information on the position, size and shape of the manganese cluster, which catalyzes water oxidation.

Original languageEnglish
Pages (from-to)739-743
Number of pages5
JournalNature
Volume409
Issue number6821
DOIs
Publication statusPublished - Feb 8 2001

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Synechococcus
Photosystem II Protein Complex
Water
Electrons
Photosystem I Protein Complex
Crystallography
Thylakoids
Protein Subunits
Photosynthesis
Manganese
Atmosphere
Protons
Electron Microscopy
X-Rays
Oxygen
Proteins

ASJC Scopus subject areas

  • General

Cite this

Zouni, A., Witt, H. T., Kern, J., Fromme, P., Krauss, N., Saenger, W., & Orth, P. (2001). Crystal structure of photosystem II from Synechococcus elongatus at 3.8 Å resolution. Nature, 409(6821), 739-743. https://doi.org/10.1038/35055589

Crystal structure of photosystem II from Synechococcus elongatus at 3.8 Å resolution. / Zouni, Athina; Witt, Horst Tobias; Kern, Jan; Fromme, Petra; Krauss, Norbert; Saenger, Wolfram; Orth, Peter.

In: Nature, Vol. 409, No. 6821, 08.02.2001, p. 739-743.

Research output: Contribution to journalArticle

Zouni, A, Witt, HT, Kern, J, Fromme, P, Krauss, N, Saenger, W & Orth, P 2001, 'Crystal structure of photosystem II from Synechococcus elongatus at 3.8 Å resolution', Nature, vol. 409, no. 6821, pp. 739-743. https://doi.org/10.1038/35055589
Zouni, Athina ; Witt, Horst Tobias ; Kern, Jan ; Fromme, Petra ; Krauss, Norbert ; Saenger, Wolfram ; Orth, Peter. / Crystal structure of photosystem II from Synechococcus elongatus at 3.8 Å resolution. In: Nature. 2001 ; Vol. 409, No. 6821. pp. 739-743.
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