Crystallization and preliminary X-ray diffraction studies of the 51 kDa protein of the mosquito-larvicidal binary toxin from Bacillus sphaericus

C. K. Chiou, E. W. Davidson, T. Thanabalu, A. G. Porter, James Paul Allen

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Certain strains of Bacillus sphaericus produce a highly toxic mosquito-larvicidal protein during sporulation which is active against vectors of dengue, encephalitis and malaria. This toxin is initially expressed as 51 and 42 kDa proteins and is converted to 43 and 39 kDa proteins, respectively, which form the active heterodimer complex. For a better understanding of the toxicity mechanism at the molecular level, the 51 kDa protein of the binary toxin of B. sphaericus strain 2297 was expressed as a glutathione-S-transferase fusion protein and purified by affinity chromatography. Protein crystals were grown from an amorphous precipitate in five months using the hanging-drop vapor-diffusion method. The protein crystals were dissolved and were found to be composed of a proteolytically modified 45.2 kDa derivative similar to the active form of this protein. The crystals form in space group P43212 (or P41212) and diffract to 2.6 Å, with unit-cell dimensions a = b = 133.48, c = 69.76 Å.

Original languageEnglish
Pages (from-to)1083-1085
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume55
Issue number5
DOIs
Publication statusPublished - May 1999

Fingerprint

Bacillus
Crystallization
Culicidae
X-Ray Diffraction
crystallization
proteins
X ray diffraction
diffraction
Proteins
x rays
Crystals
encephalitis
crystals
Affinity chromatography
glutathione
Dengue
Bacillus sphaericus binB protein
Poisons
Bacilli
Encephalitis

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

Crystallization and preliminary X-ray diffraction studies of the 51 kDa protein of the mosquito-larvicidal binary toxin from Bacillus sphaericus. / Chiou, C. K.; Davidson, E. W.; Thanabalu, T.; Porter, A. G.; Allen, James Paul.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 55, No. 5, 05.1999, p. 1083-1085.

Research output: Contribution to journalArticle

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