Crystallization and structural analysis of GADPH from Spinacia oleracea in a new form

Ana Cámara-Artigas, Masakazu Hirasawa, David B. Knaff, Meitian Wang, James Paul Allen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Two crystalline forms of GADPH (d-glyceraldehyde-3-phosphate dehydrogenase) from Spinacia oleracea were obtained using sitting-drop vapor diffusion. Despite the very low concentration of GADPH in the solutions, two crystalline forms were obtained, one of which was the previously reported C222 space group with unit-cell parameters a = 155.3, b = 181.7, c = 107.6 Å and the other of which belonged to a new space group I4122, with unit-cell parameters a = b = 120.9, c = 154.5 Å. Diffraction data were measured from both native and derivatives, yielding structures at a resolution limit of 3.0 Å. Differences at the NAD+/NADP+-binding site seen in these structures compared with the previously reported structure with bound coenzyme suggest that conformational changes associated with pyridine-nucleotide binding may play a role in the regulation of this enzyme.

Original languageEnglish
Pages (from-to)1087-1092
Number of pages6
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume62
Issue number11
DOIs
Publication statusPublished - Nov 2006

Fingerprint

Spinacia oleracea
Crystallization
structural analysis
Structural analysis
pyridine nucleotides
crystallization
Crystalline materials
coenzymes
Glyceraldehyde-3-Phosphate Dehydrogenases
dehydrogenases
Coenzymes
cells
NADP
NAD
enzymes
low concentrations
phosphates
Nucleotides
Diffraction
Binding Sites

Keywords

  • GADPH
  • Glutamate synthase
  • NAD/NADP

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Structural Biology
  • Genetics
  • Condensed Matter Physics

Cite this

Crystallization and structural analysis of GADPH from Spinacia oleracea in a new form. / Cámara-Artigas, Ana; Hirasawa, Masakazu; Knaff, David B.; Wang, Meitian; Allen, James Paul.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 62, No. 11, 11.2006, p. 1087-1092.

Research output: Contribution to journalArticle

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