Crystallization of intact and subunit L-deficient monomers from synechocystis PCC 6803 photosystem I

Petra Jekow, Wolf Dieter Schubert, Petra Fromme, Jochen Kruip, Parag R. Chitnis, Matthias Rögner, Wolfram Saenger

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Photosystem I monomers from wildtype cells of Synechocystis PCC 6803 and from a mutant deficient in the psaL gene were crystallized. PsaL encodes for the hydrophobic subunit L, which has been proposed to constitute the trimerization domain in the PS I trimer. The absence of subunit L facilitated crystallization of the PS I monomer. The unit cell dimensions and the space group for the crystals from this preparation could be determined to be a = b = 132 Å, c = 525 Å, α = β = 90°, y = 120°, the space group is P61 or P65. The results show the potential of using specifically designed deletion mutants of an integral membrane protein for the systematic improvement of crystal structure data.

Original languageEnglish
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume51
Issue number3-4
Publication statusPublished - 1996

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Synechocystis
Photosystem I Protein Complex
photosystem I
crystallization
Crystallization
Monomers
mutants
crystal structure
membrane proteins
crystals
Membrane Proteins
Genes
Crystal structure
cells
Crystals
genes

Keywords

  • Crystallization
  • Mesophilic Cyanobacterium
  • Photosystem I
  • PS I Monomers

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Crystallization of intact and subunit L-deficient monomers from synechocystis PCC 6803 photosystem I. / Jekow, Petra; Schubert, Wolf Dieter; Fromme, Petra; Kruip, Jochen; Chitnis, Parag R.; Rögner, Matthias; Saenger, Wolfram.

In: Zeitschrift fur Naturforschung - Section C Journal of Biosciences, Vol. 51, No. 3-4, 1996.

Research output: Contribution to journalArticle

Jekow, Petra ; Schubert, Wolf Dieter ; Fromme, Petra ; Kruip, Jochen ; Chitnis, Parag R. ; Rögner, Matthias ; Saenger, Wolfram. / Crystallization of intact and subunit L-deficient monomers from synechocystis PCC 6803 photosystem I. In: Zeitschrift fur Naturforschung - Section C Journal of Biosciences. 1996 ; Vol. 51, No. 3-4.
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T1 - Crystallization of intact and subunit L-deficient monomers from synechocystis PCC 6803 photosystem I

AU - Jekow, Petra

AU - Schubert, Wolf Dieter

AU - Fromme, Petra

AU - Kruip, Jochen

AU - Chitnis, Parag R.

AU - Rögner, Matthias

AU - Saenger, Wolfram

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AB - Photosystem I monomers from wildtype cells of Synechocystis PCC 6803 and from a mutant deficient in the psaL gene were crystallized. PsaL encodes for the hydrophobic subunit L, which has been proposed to constitute the trimerization domain in the PS I trimer. The absence of subunit L facilitated crystallization of the PS I monomer. The unit cell dimensions and the space group for the crystals from this preparation could be determined to be a = b = 132 Å, c = 525 Å, α = β = 90°, y = 120°, the space group is P61 or P65. The results show the potential of using specifically designed deletion mutants of an integral membrane protein for the systematic improvement of crystal structure data.

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