Crystallization of the mosquito-larvicidal binary toxin produced by Bacillus sphaericus

Alex W. Smith, Ana Camara-Artigas, James Paul Allen

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The Bacillus sphaericus binary toxin is expressed during the early stages of sporulation and is composed of two separately encoded polypeptides: BinA (41.9 kDa) and BinB (51.4 kDa). The binary toxin forms microcrystalline inclusions inside the mother cell that, once ingested, are solubilized in the alkaline pH of the larval gut. B. sphaericus cultures were grown to complete sporulation, harvested and washed. The binary toxin was solubilized with 50 mM NaOH, purified using column chromatography and crystallized. Crystals were grown using the sitting-drop vapor-diffusion method in a glycine-buffered solution containing 0.5 M NaCl and 19.5% PEG 4000 as the precipitant. Native data were collected to a resolution of 2.7 Å.

Original languageEnglish
Pages (from-to)952-953
Number of pages2
JournalActa Crystallographica Section D: Biological Crystallography
Volume60
Issue number5
DOIs
Publication statusPublished - May 2004

Fingerprint

Column chromatography
Bacillus
polypeptides
chromatography
glycine
Crystallization
Culicidae
Glycine
Polyethylene glycols
Chromatography
Stem Cells
Vapors
inclusions
vapors
crystallization
Peptides
Crystals
cells
crystals
Bacillus sphaericus binB protein

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

Crystallization of the mosquito-larvicidal binary toxin produced by Bacillus sphaericus. / Smith, Alex W.; Camara-Artigas, Ana; Allen, James Paul.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 60, No. 5, 05.2004, p. 952-953.

Research output: Contribution to journalArticle

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