Crystallization of the reaction center from Rhodobacter sphaeroides in a new tetragonal form

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Abstract

The reaction center from the nonsulfur purple bacteriumRhodobacter sphaeroideshas been crystallized in a new form. The crystals grew in the presence of polyethylene glycol 4000, the detergent β‐octyl glucoside, and the amphiphiles heptane triol and benzamidine hydrochloride, using the sitting drop method. The space group of these crystals is tetragonal, P41(43)212, and the cell constants are a = b = 141.5 Å and c = 276.7 Å with probably 2 proteins per asymmetric unit. A native data set has been set collected to a resolution of 2.8 Å consisting of 56,332 unique reflections (50,731 with F > 2σ) with anRsym of 9.5%. Analysis of the diffraction data is underway using molecular and isomorphous replacement. © 1994 Wiley‐Liss, Inc.

Original languageEnglish
Pages (from-to)283-286
Number of pages4
JournalProteins: Structure, Function, and Bioinformatics
Volume20
Issue number3
DOIs
Publication statusPublished - Nov 1994

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Keywords

  • detergent
  • membrane protein
  • photosynthesis
  • pigment‐protein complexes
  • protein structure

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

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