Crystallographic Data Support the Carousel Mechanism of Water Supply to the Oxygen-Evolving Complex of Photosystem II

Jimin Wang, Mikhail Askerka, Gary W Brudvig, Victor S. Batista

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Photosystem II (PSII) oxidizes water to produce oxygen through a four-step photocatalytic cycle. Understanding PSII structure-function relations is important for the development of biomimetic photocatalytic systems. The quantum mechanics/molecular mechanics (QM/MM) analysis of substrate water binding to the oxygen-evolving complex (OEC) has suggested a rearrangement of water ligands in a carousel mechanism around a key Mn center. Here, we find that the most recently reported X-ray free-electron laser (XFEL) crystallographic data obtained for the dark-stable S1 state and the doubly flashed S3 state at 2.25 Å resolution support the carousel mechanism. The features in the XFEL data and QM/MM model-simulated difference Fourier maps suggest that water displacement may occur from the so-called "narrow" channel, resulting in binding of a new water molecule to the OEC, and thus provide new insights into the nature of rearrangements of water ligands along the catalytic cycle before O=O bond formation.

Original languageEnglish
Pages (from-to)2299-2306
Number of pages8
JournalACS Energy Letters
Volume2
Issue number10
DOIs
Publication statusPublished - Oct 13 2017

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Photosystem II Protein Complex
Water supply
Oxygen
Water
X ray lasers
Molecular mechanics
Quantum theory
Free electron lasers
Ligands
Biomimetics
Molecules
Substrates

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Energy Engineering and Power Technology
  • Materials Chemistry

Cite this

Crystallographic Data Support the Carousel Mechanism of Water Supply to the Oxygen-Evolving Complex of Photosystem II. / Wang, Jimin; Askerka, Mikhail; Brudvig, Gary W; Batista, Victor S.

In: ACS Energy Letters, Vol. 2, No. 10, 13.10.2017, p. 2299-2306.

Research output: Contribution to journalArticle

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